High-Level Accumulation of a Recombinant Antibody Fragment in the Periplasm of Escherichia coli Requires a Triple-Mutant (degP prc spr) Host Strain

Biotechnology and Bioengineering

Volumn 85, Issue 5, 2004, Pages 463-474

  Chen, Christina ^a   Snedecor, Brad ^a   Nishihara, Julie C ^a   Joly, John C ^a   McFarland, Nancy ^a   Andersen, Dana C ^a   Battersby, John E ^a   Champion, Kathleen M ^a  

^a GENENTECH INC   (United States)

Author keywords

Biopharmaceutical; Escherichia coli; High cell density fermentation; Periplasmic protease; Recombinant antibody; Two dimensional gel electrophoresis

Indexed keywords

ANTIBODIES; DEGRADATION; ENZYMES; ESCHERICHIA COLI; FERMENTATION; GENES;

CELL DENSITY; PROTEOLYTIC DEGRADATION;

BIOTECHNOLOGY;

IMMUNOGLOBULIN F(AB')2 FRAGMENT; IMMUNOGLOBULIN LIGHT CHAIN; PERIPLASMIC PROTEIN; PROTEIN PRC; PROTEINASE; RECOMBINANT ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM MUTANT; CELL DENSITY; CYTOPLASM; DEGP GENE; ESCHERICHIA COLI; FERMENTATION; GENE MUTATION; NONHUMAN; PRC GENE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SPR GENE;

ANTIGENS, CD18; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SURVIVAL; ENDOPEPTIDASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HEAT-SHOCK PROTEINS; IMMUNOGLOBULIN FRAGMENTS; MUTAGENESIS, SITE-DIRECTED; PERIPLASM; PERIPLASMIC PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; SPECIES SPECIFICITY;

ESCHERICHIA COLI;

EID: 1442352581     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20014     Document Type: Article

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