Hematopoietic stem cell mobilization: Updated conceptual renditions

Leukemia

Volumn 27, Issue 1, 2013, Pages 24-31

  Bonig, H ^a,b   Papayannopoulou, T ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b Institute for Transfusion Medicine and Immunohematology   (Germany)

Author keywords

bone marrow; CXCR4; G CSF; hematopoietic stem cell; mobilization; niche

Indexed keywords

CHEMOKINE RECEPTOR CXCR4 ANTAGONIST; GRANULOCYTE COLONY STIMULATING FACTOR; PLERIXAFOR; STROMAL CELL DERIVED FACTOR 1;

AUTONOMIC NERVOUS SYSTEM; BLOOD FLOW; BONE MARROW; CELL PROLIFERATION; DOWN REGULATION; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; MOLECULARLY TARGETED THERAPY; NONHUMAN; OXIDATIVE STRESS; OXYGENATION; PRIORITY JOURNAL; PROGENY; REVIEW; STEADY STATE; STEM CELL MOBILIZATION; STEM CELL NICHE; TRABECULAR BONE;

ANIMALS; BONE MARROW; HEMATOPOIETIC STEM CELL MOBILIZATION; HUMANS; STEM CELL NICHE;

EID: 84873567259     PISSN: 08876924     EISSN: 14765551     Source Type: Journal    
DOI: 10.1038/leu.2012.254     Document Type: Review

References (102)

1. Broxmeyer HE, Orschell CM, Clapp DW, Hangoc G, Cooper S, Plett PA et al. Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med 2005; 201: 1307-1318.
2. Ehninger A, Trumpp A. The bone marrow stem cell niche grows up: mesenchymal stem cells and macrophages move in. J Exp Med 2011; 208: 421-428.
3. Greenbaum AM, Link DC. Mechanisms of G-CSF-mediated hematopoietic stem and progenitor mobilization. Leukemia 2011; 25: 211-217.
4. Hoggatt J, Pelus LM. Many mechanisms mediating mobilization: an alliterative review. Curr Opin Hematol 2011; 18: 231-238.
5. Levesque JP, Winkler IG. Mobilization of hematopoietic stem cells: state of the art. Curr Opin Organ Transplant 2008; 13: 53-58.
6. Mohty M, Ho AD. In and out of the niche: perspectives in mobilization of hematopoietic stem cells. Exp Hematol 2011; 39: 723-729.
7. Ratajczak MZ, Kim C. The use of chemokine receptor agonists in stem cell mobilization. Expert Opin Biol Ther 2012; 12: 287-297.
8. Ratajczak MZ, Kim CH, Wojakowski W, Janowska-Wieczorek A, Kucia M, Ratajczak J. Innate immunity as orchestrator of stem cell mobilization. Leukemia 2010; 24: 1667-1675.
9. Levesque JP, Helwani FM, Winkler IG. The endosteal 'osteoblastic' niche and its role in hematopoietic stem cell homing and mobilization. Leukemia 2010; 24: 1979-1992.
10. Ratajczak MZ. Spotlight series on stem cell mobilization: many hands on the ball, but who is the quarterback? Leukemia 2010; 24: 1665-1666.
11. Ratajczak MZ, Kim CH, bdel-Latif A, Schneider G, Kucia M, Morris AJ et al. A novel perspective on stem cell homing and mobilization: review on bioactive lipids as potent chemoattractants and cationic peptides as underappreciated modulators of responsiveness to SDF-1 gradients. Leukemia 2012; 26: 63-72.
12. Winkler IG, Pettit AR, Raggatt LJ, Jacobsen RN, Forristal CE, Barbier V et al. Hematopoietic stem cell mobilizing agents G-CSF, cyclophosphamide or AMD3100 have distinct mechanisms of action on bone marrow HSC niches and bone formation. Leukemia 2012; 26: 1594-1601.
13. Rettig MP, Ansstas G, DiPersio JF. Mobilization of hematopoietic stem and progenitor cells using inhibitors of CXCR4 and VLA-4. Leukemia 2012; 26: 34-53.
14. Ellis SL, Grassinger J, Jones A, Borg J, Camenisch T, Haylock D et al. The relationship between bone, hemopoietic stem cells, and vasculature. Blood 2011; 118: 1516-1524.
15. Sugiyama T, Kohara H, Noda M, Nagasawa T. Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches. Immunity 2006; 25: 977-988.
16. Kiel MJ, Morrison SJ. Maintaining hematopoietic stem cells in the vascular niche. Immunity 2006; 25: 862-864.
17. Kiel MJ, Morrison SJ. Uncertainty in the niches that maintain haematopoietic stem cells. Nat Rev Immunol 2008; 8: 290-301.
18. Lo CC, Fleming HE, Wu JW, Zhao CX, Miake-Lye S, Fujisaki J et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. Nature 2009; 457: 92-96.
19. Bianco P. Bone and the hematopoietic niche: a tale of two stem cells. Blood 2011; 117: 5281-5288.
20. Szabo Z, Szabo G. The effect of haemorrhage and bone fracture on bone marrow circulation. Res Exp Med (Berl) 1978; 172: 7-17.
21. Karpe F, Fielding BA, Ardilouze JL, Ilic V, Macdonald IA, Frayn KN. Effects of insulin on adipose tissue blood flow in man. J Physiol 2002; 540(Pt 3): 1087-1093.
22. Harrison JS, Rameshwar P, Chang V, Bandari P. Oxygen saturation in the bone marrow of healthy volunteers. Blood 2002; 99: 394.
23. Suda T, Takubo K, Semenza GL. Metabolic regulation of hematopoietic stem cells in the hypoxic niche. Cell Stem Cell 2011; 9: 298-310.
24. Pennings FA, Schuurman PR, van den MP, Bouma GJ. Brain tissue oxygen pressure monitoring in awake patients during functional neurosurgery: the assessment of normal values. J Neurotrauma 2008; 25: 1173-1177.
25. Levesque JP, Winkler IG, Hendy J, Williams B, Helwani F, Barbier V et al. Hematopoietic progenitor cell mobilization results in hypoxia with increased hypoxiainducible transcription factor-1 alpha and vascular endothelial growth factor A in bone marrow. Stem Cells 2007; 25: 1954-1965.
26. Van DD, Harris N. Bone marrow reactions to trauma. Stimulation of erythropoietic marrow by mechanical disruption, fracture or endosteal curettage. Blood 1969; 34: 257-275.
27. Jiang Y, Bonig H, Ulyanova T, Chang K, Papayannopoulou T. On the adaptation of endosteal stem cell niche function in response to stress. Blood 2009; 114: 3773-3782.
28. Croizat H, Frindel E, Tubiana M. The effect of partial body irradiation on haemopoietic stem cell migration. Cell Tissue Kinet 1980; 13: 319-325.
29. Murate T, Utsumi M, Hotta T, Yamada H. Hematopoietic stem cell migration and proliferation after partial body irradiation: significant role of the spleen in hematopoietic recovery. Nihon Ketsueki Gakkai Zasshi 1983; 46: 867-875.
30. Abkowitz JL, Robinson AE, Kale S, Long MW, Chen J. Mobilization of hematopoietic stem cells during homeostasis and after cytokine exposure. Blood 2003; 102: 1249-1253.
31. McBride RA, Simonsen M. Cellular and humoral phenomena during the inductive phase of parabisos tolerance. Transplantation 1965; 3: 140-154.
32. Walker DG. Osteopetrosis cured by temporary parabiosis. Science 1973; 180: 875.
33. Harris JE, Ford CE, Barnes DW, Evans EP. Evidence from parabiosis for an afferent stream of cells. Nature 1964; 201: 886-887.
34. Barisic-Dujmovic T, Boban I, Clark SH. Fibroblasts/myofibroblasts that participate in cutaneous wound healing are not derived from circulating progenitor cells. J Cell Physiol 2010; 222: 703-712.
35. Boban I, Barisic-Dujmovic T, Clark SH. Parabiosis model does not show presence of circulating osteoprogenitor cells. Genesis 2010; 48: 171-182.
36. Stute N, Fehse B, Schroder J, Arps S, Adamietz P, Held KR et al. Human mesenchymal stem cells are not of donor origin in patients with severe aplastic anemia who underwent sex-mismatched allogeneic bone marrow transplant J Hematother Stem Cell Res 2002; 11: 977-984.
37. Otsuru S, Hofmann TJ, Rasini V, Veronesi E, Dominici M, Horwitz EM. Osteopoietic engraftment after bone marrow transplantation: effect of inbred strain of mice. Exp Hematol 2010; 38: 836-844.
38. Dominici M, Marino R, Rasini V, Spano C, Paolucci P, Conte P et al. Donor cellderived osteopoiesis originates from a self-renewing stem cell with a limited regenerative contribution after transplantation. Blood 2008; 111: 4386-4391.
39. Marino R, Martinez C, Boyd K, Dominici M, Hofmann TJ, Horwitz EM. Transplantable marrow osteoprogenitors engraft in discrete saturable sites in the marrow microenvironment. Exp Hematol 2008; 36: 360-368.
40. Le BK, Gotherstrom C, Ringden O, Hassan M, McMahon R, Horwitz E et al. Fetal mesenchymal stem-cell engraftment in bone after in utero transplantation in a patient with severe osteogenesis imperfecta. Transplantation 2005; 79: 1607-1614.
41. Scopes J, Ismail M, Marks KJ, Rutherford TR, Draycott GS, Pocock C et al. Correction of stromal cell defect after bone marrow transplantation in aplastic anaemia. Br J Haematol 2001; 115: 642-652.
42. Laver J, Jhanwar SC, O'Reilly RJ, Castro-Malaspina H. Host origin of the human hematopoietic microenvironment following allogeneic bone marrow transplantation. Blood 1987; 70: 1966-1968.
43. Hollings PE, Fitzgerald PH, Heaton DC, Beard ME. Host origin of in vitro bone marrow fibroblasts after marrow transplantation in man. Int J Cell Cloning 1984; 2: 348-355.
44. Wilson FD, Konrad PN, Greenberg BR, Klein AK, Walling PA. Cytogenetic studies on bone marrow fibroblasts from a male-female hematopoietic chimera. Evidence that stromal elements in human transplantation recipients are of host type. Transplantation 1978; 25: 87-88.
45. Bonig H, Chudziak D, Priestley G, Papayannopoulou T. Insights into the biology of mobilized hematopoietic stem/progenitor cells through innovative treatment schedules of the CXCR4 antagonist AMD3100. Exp Hematol 2009; 37: 402-415.
46. King AG, Horowitz D, SB Dillon, Levin R, Farese AM, MacVittie TJ et al. Rapid mobilization of murine hematopoietic stem cells with enhanced engraftment properties and evaluation of hematopoietic progenitor cell mobilization in rhesus monkeys by a single injection of SB-251353, a specific truncated form of the human CXC chemokine GRObeta. Blood 2001; 97: 1534-1542.
47. Laterveer L, Lindley IJ, Hamilton MS, Willemze R, Fibbe WE. Interleukin-8 induces rapid mobilization of hematopoietic stem cells with radioprotective capacity and long-term myelolymphoid repopulating ability. Blood 1995; 85: 2269-2275.
48. Zhao M, Ross JT, Itkin T, Perry JM, Venkatraman A, Haug JS et al. FGF signaling facilitates post-injury recovery of mouse hematopoietic system. Blood 2012; 120: 1831-1842.
49. Dar A, Schajnovitz A, Lapid K, Kalinkovich A, Itkin T, Ludin A et al. Rapid mobilization of hematopoietic progenitors by AMD3100 and catecholamines is mediated by CXCR4-dependent SDF-1 release from bone marrow stromal cells. Leukemia 2011; 25: 1286-1296.
50. Foudi A, Jarrier P, Zhang Y, Wittner M, Geay JF, Lecluse Y et al. Reduced retention of radioprotective hematopoietic cells within the bone marrow microenvironment in CXCR4-/-chimeric mice. Blood 2006; 107: 2243-2251.
51. Nie Y, Han YC, Zou YR. CXCR4 is required for the quiescence of primitive hematopoietic cells. J Exp Med 2008; 205: 777-783.
52. Golan K, Vagima Y, Ludin A, Itkin T, Cohen-Gur S, Kalinkovich A et al. S1P promotes murine progenitor cell egress and mobilization via S1P1-mediated ROS signaling and SDF-1 release. Blood 2012; 119: 2478-2488.
53. Juarez JG, Harun N, Thien M, Welschinger R, Baraz R, Pena AD et al. Sphingosine-1-phosphate facilitates trafficking of hematopoietic stem cells and their mobilization by CXCR4 antagonists in mice. Blood 2012; 119: 707-716.
54. Ratajczak MZ, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin MJ et al. Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex. Leukemia 2010; 24: 976-985.
55. Ratajczak J, Kucia M, Mierzejewska K, Liu R, Kim CH, Natarajan N et al. A novel view of paroxysmal nocturnal hemoglobinuria pathogenesis: more motile PNH hematopoietic stem/progenitor cells displace normal HSPCs from their niches in bone marrow due to defective adhesion, enhanced migration and mobilization in response to erythrocyte-released sphingosine-1 phosphate gradient Leukemia 2012; 26: 1722-1725.
56. Papayannopoulou T, Priestley GV, Bonig H, Nakamoto B. The role of G-protein signaling in hematopoietic stem/progenitor cell mobilization. Blood 2003; 101: 4739-4747.
57. Sweeney EA, Papayannopoulou T. Increase in circulating SDF-1 after treatment with sulfated glycans. The role of SDF-1 in mobilization. Ann N Y Acad Sci 2001; 938: 48-52.
58. Sweeney EA, Lortat-Jacob H, Priestley GV, Nakamoto B, Papayannopoulou T. Sulfated polysaccharides increase plasma levels of SDF-1 in monkeys and mice: involvement in mobilization of stem/progenitor cells. Blood 2002; 99: 44-51.
59. Sweeney EA, Priestley GV, Nakamoto B, Collins RG, Beaudet AL, Papayannopoulou T. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci USA 2000; 97: 6544-6549.
60. Uy GL, Rettig MP, Motabi IH, McFarland K, Trinkaus KM, Hladnik LM et al. A phase 1/2 study of chemosensitization with the CXCR4 antagonist plerixafor in relapsed or refractory acute myeloid leukemia. Blood 2012; 119: 3917-3924.
61. van PM, van OR, Velders GA, Hagoort H, Heegaard PM, Lindley IJ et al. Serpina1 is a potent inhibitor of IL-8-induced hematopoietic stem cell mobilization. Proc Natl Acad Sci USA 2006; 103: 1469-1474.
62. Levesque JP, Hendy J, Takamatsu Y, Simmons PJ, Bendall LJ. Disruption of the CXCR4/CXCL12 chemotactic interaction during hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide. J Clin Invest 2003; 111: 187-196.
63. Liu F, Poursine-Laurent J, Link DC. The granulocyte colony-stimulating factor receptor is required for the mobilization of murine hematopoietic progenitors into peripheral blood by cyclophosphamide or interleukin-8 but not flt-3 ligand. Blood 1997; 90: 2522-2528.
64. Christopher MJ, Liu F, Hilton MJ, Long F, Link DC. Suppression of CXCL12 production by bone marrow osteoblasts is a common and critical pathway for cytokine-induced mobilization. Blood 2009; 114: 1331-1339.
65. Nagasawa T, Omatsu Y, Sugiyama T. Control of hematopoietic stem cells by the bone marrow stromal niche: the role of reticular cells. Trends Immunol 2011; 32: 315-320.
66. Mendez-Ferrer S, Michurina TV, Ferraro F, Mazloom AR, Macarthur BD, Lira SA et al. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 2010; 466: 829-834.
67. Eash KJ, Greenbaum AM, Gopalan PK, Link DC. CXCR2 and CXCR4 antagonistically regulate neutrophil trafficking from murine bone marrow. J Clin Invest 2010; 120: 2423-2431.
68. Semerad CL, Christopher MJ, Liu F, Short B, Simmons PJ, Winkler I et al. G-CSF potently inhibits osteoblast activity and CXCL12 mRNA expression in the bone marrow. Blood 2005; 106: 3020-3027.
69. Kollet O, Shivtiel S, Chen YQ, Suriawinata J, Thung SN, Dabeva MD et al. HGF SDF-1, and MMP-9 are involved in stress-induced human CD34\+ stem cell recruitment to the liver. J Clin Invest 2003; 112: 160-169.
70. Abbott JD, Huang Y, Liu D, Hickey R, Krause DS, Giordano FJ. Stromal cell-derived factor-1alpha plays a critical role in stem cell recruitment to the heart after myocardial infarction but is not sufficient to induce homing in the absence of injury. Circulation 2004; 110: 3300-3305.
71. Bonig H, Priestley GV, Papayannopoulou T. Hierarchy of molecular-pathway usage in bone marrow homing and its shift by cytokines. Blood 2006; 107: 79-86.
72. Wiesmann A, Spangrude GJ. Marrow engraftment of hematopoietic stem and progenitor cells is independent of Galphai-coupled chemokine receptors. Exp Hematol 1999; 27: 946-955.
73. Peled A, Petit I, Kollet O, Magid M, Ponomaryov T, Byk T et al. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 1999; 283: 845-848.
74. Bonig H, Priestley GV, Nilsson LM, Jiang Y, Papayannopoulou T. PTX-sensitive signals in bone marrow homing of fetal and adult hematopoietic progenitor cells. Blood 2004; 104: 2299-2306.
75. Sanchez-Aguilera A, Lee YJ, Lo CC, Ferraro F, Brumme K, Mondal S et al. Guanine nucleotide exchange factor Vav1 regulates perivascular homing and bone marrow retention of hematopoietic stem and progenitor cells. Proc Natl Acad Sci USA 2011; 108: 9607-9612.
76. Cancelas JA, Jansen M, Williams DA. The role of chemokine activation of Rac GTPases in hematopoietic stem cell marrow homing, retention, and peripheral mobilization. Exp Hematol 2006; 34: 976-985.
77. Bonig H, Watts KL, Chang KH, Kiem HP, Papayannopoulou T. Concurrent blockade of alpha4-integrin and CXCR4 in hematopoietic stem/progenitor cell mobilization. Stem Cells 2009; 27: 836-837.
78. Ramirez P, Rettig MP, Uy GL, Deych E, Holt MS, Ritchey JK et al. BIO5192, a small molecule inhibitor of VLA-4, mobilizes hematopoietic stem and progenitor cells. Blood 2009; 114: 1340-1343.
79. Scott LM, Priestley GV, Papayannopoulou T. Deletion of alpha4 integrins from adult hematopoietic cells reveals roles in homeostasis, regeneration, and homing. Mol Cell Biol 2003; 23: 9349-9360.
80. Kew RR, Penzo M, Habiel DM, Marcu KB. The IKKalpha-dependent NF-kappaB p52/RelB noncanonical pathway is essential to sustain a CXCL12 autocrine loop in cells migrating in response to HMGB1. J Immunol 2012; 188: 2380-2386.
81. Kawabata K, Ujikawa M, Egawa T, Kawamoto H, Tachibana K, Iizasa H et al. A cellautonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution. Proc Natl Acad Sci USA 1999; 96: 5663-5667.
82. Lapidot T, Petit I. Current understanding of stem cell mobilization: the roles of chemokines, proteolytic enzymes, adhesion molecules, cytokines, and stromal cells. Exp Hematol 2002; 30: 973-981.
83. Fruehauf S, Seggewiss R. It's moving day: factors affecting peripheral blood stem cell mobilization and strategies for improvement [corrected]. Br J Haematol 2003; 122: 360-375.
84. Cashen AF, Link D, Devine S, DiPersio J. Cytokines and stem cell mobilization for autologous and allogeneic transplantation. Curr Hematol Rep 2004; 3: 406-412.
85. Levesque JP, Hendy J, Takamatsu Y, Williams B, Winkler IG, Simmons PJ. Mobilization by either cyclophosphamide or granulocyte colony-stimulating factor transforms the bone marrow into a highly proteolytic environment. Exp Hematol 2002; 30: 440-449.
86. Thomas J, Liu F, Link DC. Mechanisms of mobilization of hematopoietic progenitors with granulocyte colony-stimulating factor. Curr Opin Hematol 2002; 9: 183-189.
87. Elfenbein GJ, Sackstein R. Primed marrow for autologous and allogeneic transplantation: a review comparing primed marrow to mobilized blood and steadystate marrow. Exp Hematol 2004; 32: 327-339.
88. Levesque JP, Barbier V, Nowlan B, McCarhty D, Winkler I. Impairment of hematopoietic stem cell (HSC) niche by G-CSF is associated with rapid mobilization of serially reconstituting HSC and reduced competitive repopulation of mobilized bone marrow. Blood 2011; 118a: 1889.
89. Katayama Y, Battista M, Kao WM, Hidalgo A, Peired AJ, Thomas SA et al. Signals from the sympathetic nervous system regulate hematopoietic stem cell egress from bone marrow. Cell 2006; 124: 407-421.
90. Lucas D, Battista M, Shi PA, Isola L, Frenette PS. Mobilized hematopoietic stem cell yield depends on species-specific circadian timing. Cell Stem Cell 2008; 3: 364-366.
91. Lucas D, Bruns I, Battista M, Mendez-Ferrer S, Magnon C, Kunisaki Y et al. Norepinephrine reuptake inhibition promotes mobilization in mice: potential impact to rescue low stem cell yields. Blood 2012; 119: 3962-3965.
92. Mueller MM, Bialleck H, Bomke B, Brauninger S, Varga C, Seidl C et al. Safety and efficacy of healthy volunteer stem cell mobilization with filgrastim G-CSF and mobilized stem cell apheresis: esults of a prospective longitudinal 5-year followup study. Vox Sang 2012; e-pub ahead of print 24 July 2012; PMID 22827736.
93. Holig K, Kramer M, Kroschinsky F, Bornhauser M, Mengling T, Schmidt AH et al. Safety and efficacy of hematopoietic stem cell collection from mobilized peripheral blood in unrelated volunteers: 12 years of single-center experience in 3928 donors. Blood 2009; 114: 3757-3763.
94. Winkler IG, Levesque JP. Mechanisms of hematopoietic stem cell mobilization: when innate immunity assails the cells that make blood and bone. Exp Hematol 2006; 34: 996-1009.
95. Bonig H, Wundes A, Chang KH, Lucas S, Papayannopoulou T. Increased numbers of circulating hematopoietic stem/progenitor cells are chronically maintained in patients treated with the CD49d blocking antibody natalizumab. Blood 2008; 111: 3439-3441.
96. Broxmeyer HE, Kim CH. Regulation of hematopoiesis in a sea of chemokine family members with a plethora of redundant activities. Exp Hematol 1999; 27: 1113-1123.
97. Jalili A, Shirvaikar N, Marquez-Curtis L, Qiu Y, Korol C, Lee H et al. Fifth complement cascade protein (C5) cleavage fragments disrupt the SDF-1/CXCR4 axis: further evidence that innate immunity orchestrates the mobilization of hematopoietic stem/progenitor cells. Exp Hematol 2010; 38: 321-332.
98. Lee HM, Wysoczynski M, Liu R, Shin DM, Kucia M, Botto M et al. Mobilization studies in complement-deficient mice reveal that optimal AMD3100 mobilization of hematopoietic stem cells depends on complement cascade activation by AMD3100-stimulated granulocytes. Leukemia 2010; 24: 573-582.
99. Reca R, Cramer D, Yan J, Laughlin MJ, Janowska-Wieczorek A, Ratajczak J et al. A novel role of complement in mobilization: immunodeficient mice are poor granulocyte-colony stimulating factor mobilizers because they lack complement-activating immunoglobulins. Stem Cells 2007; 25: 3093-3100.
100. Ratajczak MZ, Kim C, Wu W, Shin DM, Bryndza E, Kucia M et al. The role of innate immunity in trafficking of hematopoietic stem cells-an emerging link between activation of complement cascade and chemotactic gradients of bioactive sphingolipids. Adv Exp Med Biol 2012; 946: 37-54.
101. Janowska-Wieczorek A, Marquez-Curtis LA, Shirvaikar N, Ratajczak MZ. The role of complement in the trafficking of hematopoietic stem/progenitor cells. Transfusion 2012; e-pub ahead of print 9th April 2012; PMID 22486360.
102. Dauber K, Becker D, Odendahl M, Seifried E, Bonig H, Tonn T. Enumeration of viable CD34(\+) cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD stem cell enumeration kit. Cytotherapy 2011; 13: 449-458.