Enumeration of viable CD34+ cells by flow cytometry in blood, bone marrow and cord blood: Results of a study of the novel BD™â., ¢ stem cell enumeration kit

Cytotherapy

Volumn 13, Issue 4, 2011, Pages 449-458

  Dauber, Katrin ^a   Becker, Daniela ^a   Odendahl, Marcus ^a   Seifried, Erhard ^a   Bonig, Halvard ^a   Tonn, Torsten ^a,b,c  

^a GOETHE UNIVERSITY   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c German Red Cross Blood Donation Service East   (Germany)

Author keywords

flow cytometry; International Society of Hematotherapy and Graft Engineering; stem cell enumeration; viability dye 7 AAD

Indexed keywords

ARTICLE; BLOOD; BONE MARROW; CD34+ CELL; CELL ASSAY; CELL COUNT; CELL VIABILITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LEUKAPHERESIS; LEUKOCYTE; PRIORITY JOURNAL; STEM CELL; UMBILICAL CORD;

EID: 79952671901     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.3109/14653249.2010.529894     Document Type: Article

References (25)

1. Armitage S, Hargreaves R, Samson D, Brennan M, Kanfer E, Navarrete C. CD34 counts to predict the adequate collection of peripheral blood progenitor cells. Bone Marrow Transplant. 1997;20:587-91. (Pubitemid 27464567)
2. Haas R, Mohle R, Fruhauf S, Goldschmidt H, Witt B, Flentje M, et al. Patient characteristics associated with successful mobilizing and autografting of peripheral blood progenitor cells in malignant lymphoma. Blood. 1994;83: 3787-94. (Pubitemid 24175570)
3. Zaucha JM, Gooley T, Bensinger WI, Heimfeld S, Chauncey TR, Zaucha R, et al. CD34 cell dose in granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cell grafts affects engraftment kinetics and development of extensive chronic graft-versus-host disease after human leukocyte antigen-identical sibling transplantation. Blood. 2001; 98:3221-7.
4. + cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering. Cytometry. 1998; 34:61-70.
5. + cell doses are associated with decreased relapse rates. Biol Blood Marrow Transplant. 2008;14:449-57. (Pubitemid 351372753)
6. + cell dose. Blood. 2009;114:2606-16.
7. Weaver CH, Hazelton B, Birch R, Palmer P, Allen C, Schwartz-berg L, et al. An analysis of engraftment kinetics as a function of the CD34 content of peripheral blood progenitor cell collections in 692 patients after the administration of myeloablative chemotherapy. Blood. 1995;86:3961-9.
8. Refaai MA, Cusick RM, Paranjape G, Sutor LJ. Low CD34 collection from a healthy blood progenitor cell donor: a case report. J Clin Apher. 2009;24:262-4.
9. + cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother. 1996;5: 213-26.
10. Sutherland DR, Nayyar R, Acton E, Giftakis A, Dean S, Mosiman VL. Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers. Cytotherapy. 2009;11: 595-605.
11. + hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis. Cytometry. 1998;34: 128-42.
12. European Directorate of the Quality of Medicines and Healthcare. European Pharmacopoeia 6th edition. 2010.
13. + hematopoietic progenitor cells Cytometry. 1998;34:264-71.
14. + cell enumeration. J Hematother Stem Cell Res. 1999;8:327-9.
15. + Haematopoietic Stem Cell Working Party. General Haematology Task Force of the British Committee for Standards in Haematology. Clin Lab Haematol. 1999;21:301-8. (Pubitemid 30007091)
16. + cell enumeration. Cytotherapy. 2004;6:50-61. (Pubitemid 38325119)
17. + hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis. Cytometry. 1998;34: 128-42.
18. Keeney M, Gratama JW, Sutherland DR. Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts. Cytometry A. 2004;58:72-5. (Pubitemid 38337833)
19. + cells after cryopreservation and comparison with trypan blue exclusion staining. Transfusion. 2005;45:1208-13. (Pubitemid 40935022)
20. + cell quantitation: an Australian multicenter study. Australasian BMT Scientists Study Group. J Hematother. 1996;5:605-16.
21. + stem and progenitor cells by flow cytometry: time to let go! Cytometry. 1998;34:280-3. (Pubitemid 29044243)
22. Civin CI, Banquerigo ML, Strauss LC, Loken MR. Antigenic analysis of hematopoiesis. VI. Flow cytometric characterization of My-10-positive progenitor cells in normal human bone marrow. Exp Hematol. 1987;15:10-7. (Pubitemid 17029120)
23. Krause DS, Ito T, Fackler MJ, Smith OM, Collector MI, Sharkis SJ, et al. Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells. Blood. 1994;84: 691-701. (Pubitemid 24223862)
24. Loken MR, Herzenber LA. Analysis of cell populations with a fluorescence-activated cell sorter. Ann NY Acad Sci. 1975;254: 163-71.
25. + cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry. 2001;46:254-61. (Pubitemid 32899797)