Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids

PLoS ONE

Volumn 7, Issue 3, 2012, Pages

  Lee, Ka Young ^a   Lee, Kyung Ho ^a   Park, Ji Woong ^b   Kim, Dong Myung ^a  

^a Chungnam National University   (South Korea)

^b Gwangju Institute of Science and Technology (GIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; MICROSPHERE;

ARTICLE; CELL FREE PROTEIN SYNTHESIS; CONJUGATION; CONTROLLED STUDY; DNA TEMPLATE; GENE EXPRESSION; MAGNETIC BEAD IMMOBILIZED PLASMID; PLASMID; PROTEIN SYNTHESIS; CELL FREE SYSTEM; GENETIC PROCEDURES; GENETICS; METABOLISM;

CELL-FREE SYSTEM; GENETIC TECHNIQUES; GREEN FLUORESCENT PROTEINS; MICROSPHERES; PLASMIDS; PROTEIN BIOSYNTHESIS;

EID: 84859043744     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0034429     Document Type: Article

References (18)

1. Rungpragayphan S, Nakano H, Yamane T, (2003) PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries. FEBS Lett 540: 147-150.
2. Kigawa T, Yamaguchi-Nunokawa E, Kodama K, Matsuda T, Yabuki T, et al. (2002) Selenomethionine incorporation into a protein by cell-free synthesis. J Struct Funct Genomics 2: 29-35.
3. Torizawa T, Shimizu M, Taoka M, Miyano H, Kainosho M, (2004) Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol. J Biomol NMR 30: 311-325.
4. Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, et al. (2004) Self-assembling protein microarrays. Science 305: 86-90.
5. Keum JW, Ahn JH, Kang TJ, Kim DM, (2009) Combinatorial, selective and reversible control of gene expression using oligodeoxynucleotides in a cell-free protein synthesis system. Biotechnol Bioeng 102: 577-582.
6. Park S, Hamad-Schifferli K, (2010) Enhancement of in vitro translation by gold nanoparticle-DNA conjugates. ACS Nano 4: 2555-2560.
7. Ahn JH, Kang TJ, Kim DM, (2008) Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system. Biotechnol Bioeng 101: 422-427.
8. Kim TW, Oh IS, Keum JW, Kwon YC, Byun JY, et al. (2007) Prolonged cell-free protein synthesis using dual energy sources: Combined use of creatine phosphate and glucose for the efficient supply of ATP and retarded accumulation of phosphate. Biotechnol Bioeng 97: 1510-1515.
9. Kim HC, Kim TW, Kim DM, (2011) Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source. Process Biochemistry 46: 1366-1369.
10. Trotta E, (2011) The 3-base periodicity and codon usage of coding sequences are correlated with gene expression at the level of transcription elongation. PLoS ONE 6: e21590.
11. Carlini DB, Chen Y, Stephan W, (2001) The relationship between third-codon position nucleotide content, codon bias, mRNA secondary structure and gene expression in the drosophilid alcohol dehydrogenase genes Adh and Adhr. Genetics 159: 623-633.
12. Chursov A, Walter MC, Schmidt T, Mironov A, Shneider A, et al. (2011) Sequence-structure relationships in yeast mRNAs. Nucleic Acids Res In press.
13. Keum JW, Ahn JH, Choi CY, Lee KH, Kwon YC, et al. (2006) The presence of a common downstream box enables the simultaneous expression of multiple proteins in an E. coli extract. Biochem Biophys Res Commun 350: 562-567.
14. Ahn JH, Keum JW, Kim DM, (2008) High-throughput, combinatorial engineering of initial codons for tunable expression of recombinant proteins. J Proteome Res 7: 2107-2113.
15. Kim TW, Keum JW, Oh IS, Choi CY, Park CG, et al. (2006) Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system. J Biotechnol 126: 554-561.
16. Zhang Y, Price BD, Tetradis S, Chakrabarti S, Maulik G, et al. (2001) Reproducible and inexpensive probe preparation for oligonucleotide arrays. Nucleic Acids Res 29: e66.
17. Scaffidi JP, Gregas MK, Lauly B, Zhang Y, Vo-Dinh T, (2011) Activity of psoralen-functionalized nanoscintillators against cancer cells upon X-ray excitation. ACS Nano 5: 4679-4687.
18. Schägger H, (2006) Tricine-SDS-PAGE. Nat Protoc 1: 16-22.