Continuous cell-free protein synthesis using glycolytic intermediates as energy sources

Journal of Microbiology and Biotechnology

Volumn 18, Issue 5, 2008, Pages 885-888

  Kim, Ho Cheol ^a   Kim, Tae Wan ^b   Park, Chang Gil ^a   Oh, In Seok ^b   Park, Kyungmoon ^c   Kim, Dong Myung ^a  

^a Chungnam National University   (South Korea)

^b Seoul National University   (South Korea)

^c Hong Ik University   (South Korea)

Author keywords

ATP regeneration; Cell free protein synthesis; Fructose 1,6 bisphosphate; Glycolysis; S12 extract

Indexed keywords

ADENOSINE TRIPHOSPHATE; FRUCTOSE 1,6 BISPHOSPHATE; PHOSPHOENOLPYRUVATE;

ARTICLE; BIOTECHNOLOGICAL PROCEDURES; BIOTECHNOLOGICAL PRODUCTION; CHEMICAL REACTION; CONTINUOUS CELL FREE PROTEIN SYNTHESIS; CONTROLLED STUDY; ENERGY RESOURCE; GLYCOLYSIS; INTERMETHOD COMPARISON; NONHUMAN; PROTEIN SYNTHESIS; REGENERATION;

ADENOSINE TRIPHOSPHATE; BIOELECTRIC ENERGY SOURCES; ENERGY-GENERATING RESOURCES; ESCHERICHIA COLI; GLUCOSE; GLYCOLYSIS; PROTEIN BIOSYNTHESIS;

EID: 50249159403     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Calhoun, K. A. and J. R. Swartz, 2005. An economical method, for cell-free protein synthesis using glucose and nucleoside monophosphates. Biotechnol. Prog. 21: 1146-1153.
2. Fedrico, K., C. Geoffrey, and K. Wieslaw. 2005. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23: 150-156.
3. Hahn, G. H. and D. M. Kim. 2006. Production of milligram quantities of recombinant proteins from PCR-amplified DNAs in a continuous-exchange cell-free protein synthesis system. Anal. Biochem. 355: 151-153.
4. Kigawa, T., T. Yabuki, Y. Yoshida, M. Tsutsui, Y. Ito, T. Shibata, and S. Yokoyama. 1999. Cell-free production and stable-isotope labeling of milligram quantities of proteins. FEBS Lett. 442: 15-19.
5. Kim, D. M. and C. Y. Choi. 1996. A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane. Biotechnol. Prog. 12: 645-649.
6. Kim, D. M. and J. R. Swartz. 2000. Prolonging cell-free protein synthesis by selective reagent additions. Biotechnol. Prog. 16: 385-390.
7. Kim, D. M., C. Y. Chio, J. H. Ahn, T. W. Kim, N. Y. Kim, I. S. Oh, and C. G. Park. 2006. Development of a rapid and productive cell-free protein synthesis system. Biotechnol. Bioprocess Eng. 11: 235-239.
8. Kim, R. G. and C. Y. Choi. 2000. Expression-independent consumption of substrates in cell-free expression system for Escherichia coli. J. Biotechnol. 84: 27-32.
9. Kim, T. W., J. W. Keum, I. S. Oh, C. Y. Choi, C. G. Park, and D. M. Kim. 2006. Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system. J. Biotechnol. 126: 554-561.
10. Kim, T. W., J. W. Keum, I. S. Oh, C. Y. Choi, H. C. Kim, and D. M. Kim. 2007. An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source. J. Biotechnol. 130: 389-393.
11. Kitaoka, Y., N. Nishimura, and M. Niwano. 1996. Cooperativity of stabilized mRNA and enhanced translation activity in the cell-free system. J. Biotechnol. 48: 1-8.
12. Lee, S. G., Y. J. Kim, S. I. Han, Y. K. Oh, S. H. Park, Y. H. Kim, and K. S. Hwang. 2006. Simulation of dynamic behavior of glucose- and tryptophan-grown Escherichia coli using constraint-based metabolic models with a hierarchical regulatory network. J. Microbiol. Biotechnol. 16: 993-998.
13. Nakano, H., T. Shinbata, R. Okumura, S. Sekiguchi, M. Fujishiro, and T. Yamane. 1998. Efficient coupled transcription/translation from PCR template by a hollow-fiber membrane bioreactor. Biotechnol. Bioeng. 64: 194-199.
14. Oh, M. K., M. J. Cha, S. G. Lee, L. Rohlin, and J. C. Liao, 2006. Dynamic gene expression profiling of Escherichia coli in carbon source transition from glucose to acetate. J. Microbiol. Biotechnol. 16: 543-549.
15. Sawasaki, T., Y. Hasegawa, M. Tsuchimochi, N. Kamura, T. Ogasawara, T. Kuroita, and Y. Endo. 2002. A bilayer cell-free protein synthesis system for high-throughput screening of gene products. FEBS Lett. 514: 102-105.
16. Spirin, A. S., V. I. Baranov, L. A. Ryabova, S. Y. Ovodov, and Y. B. Alakhov. 1988. A continuous cell-free translation system capable of producing polypeptides in high yields. Science 242: 1162-1164.
17. Spirin, A. S. 2004. High-throughput cell-free systems for synthesis of functionally active proteins. Trends Biotechnol. 22: 538-545.
18. Swartz, J. R. 2001. Advances in Escherichia coli production of therapeutic proteins. Curr. Opin. Biotechnol. 12: 195-201.