Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase

Applied Microbiology and Biotechnology

Volumn 95, Issue 6, 2012, Pages 1491-1500

  Cecchini, Davide A ^a   Pavesi, Roberto ^b   Sanna, Sara ^b   Daly, Simona ^c   Xaiz, Roberto ^c   Pregnolato, Massimo ^a   Terreni, Marco ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b AC Milan   (Italy)

^c GNOSIS SpA   (Italy)

Author keywords

Biocatalyst; Cephalosporins; Enzyme immobilization; Enzyme mutagenesis; Penicillin acylase

Indexed keywords

CEPHALOSPORINS; INDUSTRIAL SUPPORTS; KINETICALLY CONTROLLED; LARGE SCALE SYNTHESIS; PENICILLIN ACYLASE; SHORT REACTION TIME; SITE DIRECTED MUTAGENESIS; SYNTHETIC ACTIVITIES;

ANTIBIOTICS; BIOCATALYSTS; ESCHERICHIA COLI; HYDROLYSIS; MUTAGENESIS; STABILITY;

ENZYME IMMOBILIZATION;

BACTERIAL ENZYME; CEFACLOR; CEFALEXIN; CEFPROZIL; CEPHALOSPORIN DERIVATIVE; PENICILLIN ACYLASE; PENICILLIN DERIVATIVE; PENICILLIN G; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; ESCHERICHIA COLI PROTEIN; IMMOBILIZED ENZYME; PENICILLIN AMIDASE;

CATALYSIS; COLIFORM BACTERIUM; ENZYME ACTIVITY; IMMOBILIZATION; MUTAGENICITY; PERFORMANCE ASSESSMENT; REACTION KINETICS;

ARTICLE; BIOCATALYST; CHEMICAL STRUCTURE; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; HYDROLYSIS; MUTAGENESIS; NONHUMAN; PH; REACTION TIME; SITE DIRECTED MUTAGENESIS; BIOCATALYSIS; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; EVALUATION; GENETICS; KINETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; PROTEIN ENGINEERING;

ESCHERICHIA COLI;

ANTI-BACTERIAL AGENTS; BIOCATALYSIS; CEPHALOSPORINS; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; INDUSTRIAL MICROBIOLOGY; KINETICS; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PENICILLIN AMIDASE; PROTEIN ENGINEERING;

EID: 84872010935     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3817-y     Document Type: Article

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