Fusion of the Dhfr/Mtx and IR/MAR Gene Amplification Methods Produces a Rapid and Efficient Method for Stable Recombinant Protein Production

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Noguchi, Chiemi ^a   Araki, Yoshio ^a   Miki, Daisuke ^b   Shimizu, Noriaki ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b TOSOH CORPORATION   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN; METHOTREXATE; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL ISOLATION; DHFR GENE; DNA REPLICATION; FEMALE; GENE AMPLIFICATION; GENE FUSION; GENETIC TRANSFECTION; IMMUNE RESPONSE GENE; MAR GENE; MATRIX ATTACHMENT REGION; MOLECULAR CLONING; NONHUMAN; PLASMID; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

ANIMALS; CHO CELLS; CRICETINAE; GENE AMPLIFICATION; GREEN FLUORESCENT PROTEINS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RECEPTORS, FC; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84871810604     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0052990     Document Type: Article

References (35)

1. Savelyeva L, Schwab M, (2001) Amplification of oncogenes revisited: from expression profiling to clinical application. Cancer Lett 167: 115-123.
2. Albertson DG, Collins C, McCormick F, Gray JW, (2003) Chromosome aberrations in solid tumors. Nat Genet 34: 369-376.
3. Albertson DG (2006) Gene amplification in cancer. Trends Genet 22: 447-455. Epub 2006 Jun 2019.
4. Hakala MT, (1957) Prevention of toxicity of amethopterin for sarcoma-180 cells in tissue culture. Science 126: 255.
5. Omasa T, (2002) Gene Amplification and Its Application in Cell and Tissue Engineering. J BIOSCIENCE AND BIOENGINEERING 94: 600-605.
6. Mondello C, Smirnova A, Giulotto E, (2010) Gene amplification, radiation sensitivity and DNA double-strand breaks. Mutat Res 704: 29-37.
7. Wurm FM, (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22: 1393-1398.
8. Jun SC, Kim MS, Baik JY, Hwang SO, Lee GM, (2005) Selection strategies for the establishment of recombinant Chinese hamster ovary cell line with dihydrofolate reductase-mediated gene amplification. Appl Microbiol Biotechnol 69: 162-169.
9. Chusainow J, Yang YS, Yeo JH, Toh PC, Asvadi P, et al. (2009) A study of monoclonal antibody-producing CHO cell lines: what makes a stable high producer? Biotechnol Bioeng 102: 1182-1196.
10. Kim M, O'Callaghan PM, Droms KA, James DC (2011) A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies. Biotechnol Bioeng.
11. Dorai H, Corisdeo S, Ellis D, Kinney C, Chomo M, et al. (2012) Early prediction of instability of chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins. Biotechnol Bioeng 109: 1016-1030.
12. Jun SC, Kim MS, Hong HJ, Lee GM, (2006) Limitations to the Development of Humanized Antibody Producing Chinese Hamster Ovary Cells Using Glutamine Synthetase-Mediated Gene Amplification. Biotechnol Prog 22: 770-780.
13. Shimizu N, Miura Y, Sakamoto Y, Tsutsui K, (2001) Plasmids with a mammalian replication origin and a matrix attachment region initiate the event similar to gene amplification. Cancer Res 61: 6987-6990.
14. Shimizu N, Hashizume T, Shingaki K, Kawamoto JK, (2003) Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription. Cancer Res 63: 5281-5290.
15. Hashizume T, Shimizu N, (2007) Dissection of mammalian replicators by a novel plasmid stability assay. J Cell Biochem 101: 552-565.
16. Shimizu N, Misaka N, Utani K, (2007) Nonselective DNA damage induced by a replication inhibitor results in the selective elimination of extrachromosomal double minutes from human cancer cells. Genes Chromosomes Cancer 46: 865-874.
17. Shimizu N, Shingaki K, Kaneko-Sasaguri Y, Hashizume T, Kanda T, (2005) When, where and how the bridge breaks: anaphase bridge breakage plays a crucial role in gene amplification and HSR generation. Exp Cell Res 302: 233-243.
18. Harada S, Sekiguchi N, Shimizu N, (2011) Amplification of a plasmid bearing a mammalian replication initiation region in chromosomal and extrachromosomal contexts. Nucleic Acids Res 39: 958-969.
19. Araki Y, Hamafuji T, Noguchi C, Shimizu N, (2012) Efficient Recombinant Production in Mammalian Cells Using a Novel IR/MAR Gene Amplification Method. PLoS One 7: e41787.
20. Shimizu N, (2009) Extrachromosomal double minutes and chromosomal homogeneously staining regions as probes for chromosome research. Cytogenet Genome Res 124: 312-326.
21. Shimizu N, Hanada N, Utani K, Sekiguchi N, (2007) Interconversion of intra- and extra-chromosomal sites of gene amplification by modulation of gene expression and DNA methylation. J Cell Biochem 102: 515-529.
22. Yoshimura H, Sekine S, Adachi H, Uematsu Y, Mitani A, et al. (2011) High levels of human recombinant cyclooxygenase-1 expression in mammalian cells using a novel gene amplification method. Protein Expr Purif 80: 41-46.
23. Asaoka Y, Tanaka T, Tsumoto K, Tomita M, Ide T, (2012) Efficient expression of recombinant soluble human FcgammaRI in mammalian cells and its characterization. Protein Expr Purif 82: 155-161.
24. Wurm FM, Hacker D, (2011) First CHO genome. Nat Biotechnol 29: 718-720.
25. Lorico A, Toffoli G, Boiocchi M, Erba E, Broggini M, et al. (1988) Accumulation of DNA strand breaks in cells exposed to methotrexate or N10-propargyl-5,8-dideazafolic acid. Cancer Res 48: 2036-2041.
26. Martin SA, McCarthy A, Barber LJ, Burgess DJ, Parry S, et al. (2009) Methotrexate induces oxidative DNA damage and is selectively lethal to tumour cells with defects in the DNA mismatch repair gene MSH2. EMBO Mol Med 1: 323-337.
27. Pallavicini MG, DeTeresa PS, Rosette C, Gray JW, Wurm FM, (1990) Effects of methotrexate on transfected DNA stability in mammalian cells. Mol Cell Biol 10: 401-404.
28. Zahn-Zabal M, Kobr M, Girod PA, Imhof M, Chatellard P, et al. (2001) Development of stable cell lines for production or regulated expression using matrix attachment regions. J Biotechnol 87: 29-42.
29. Harraghy N, Regamey A, Girod PA, Mermod N, (2011) Identification of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections. J Biotechnol 154: 11-20.
30. Kim JM, Kim JS, Park DH, Kang HS, Yoon J, et al. (2004) Improved recombinant gene expression in CHO cells using matrix attachment regions. J Biotechnol 107: 95-105.
31. Wang F, Wang TY, Tang YY, Zhang JH, Yang XJ, (2012) Different matrix attachment regions flanking a transgene effectively enhance gene expression in stably transfected Chinese hamster ovary cells. Gene 500: 59-62.
32. Fu H, Wang L, Lin CM, Singhania S, Bouhassira EE, et al. (2006) Preventing gene silencing with human replicators. Nat Biotechnol 24: 572-576. Epub 2006 Apr 2009.
33. Kwaks TH, Barnett P, Hemrika W, Siersma T, Sewalt RG, et al. (2003) Identification of anti-repressor elements that confer high and stable protein production in mammalian cells. Nat Biotechnol 21: 553-558.
34. Running Deer J, Allison DS, (2004) High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene. Biotechnol Prog 20: 880-889.
35. Matasci M, Baldi L, Hacker DL, Wurm FM, (2011) The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability. Biotechnol Bioeng 108: 2141-2150.