The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

Biotechnology and Bioengineering

Volumn 108, Issue 9, 2011, Pages 2141-2150

  Matasci, Mattia ^a   Baldi, Lucia ^a   Hacker, David L ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

PiggyBac; Recombinant protein; Stable cell line; Transfection; Tumor necrosis factor receptor

Indexed keywords

PIGGYBAC; RECOMBINANT PROTEIN; STABLE CELL LINES; TRANSFECTION; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL CELL CULTURE; CYTOLOGY; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER; GLYCOPROTEINS; INTEGRATION; LAKES; MACROPHAGES; MAMMALS; PRODUCTIVITY; PROTEINS; STANDARDS; TUMORS;

CLONE CELLS;

RECOMBINANT PROTEIN; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL CELL; ARTICLE; CELL LINE; CELL POPULATION; CELL SCREENING; CELL SELECTION; CHO CELL; DNA TRANSPOSITION; FEMALE; GENE EXPRESSION; GENE TRANSFER; GENETIC SELECTION; GENETIC TRANSFECTION; MOLECULAR CLONING; NONHUMAN; PIGGYBAC; SUSPENSION CELL CULTURE; TRANSGENE; TRANSPOSON;

ANIMALS; BIOTECHNOLOGY; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; DNA TRANSPOSABLE ELEMENTS; GENE DOSAGE; GENE TRANSFER TECHNIQUES; HUMANS; IMMUNOGLOBULIN G; PLASMIDS; RECEPTORS, TUMOR NECROSIS FACTOR, TYPE II; RECOMBINANT FUSION PROTEINS; TRANSGENES;

CRICETULUS GRISEUS; MAMMALIA;

EID: 79960470348     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23167     Document Type: Article

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