Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Anangi, Raveendra ^a   Koshy, Shyny ^b   Huq, Redwan ^b   Beeton, Christine ^b   Chuang, Woei Jer ^c   King, Glenn F ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

^c NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AGITOXIN 2; DISULFIDE; HYBRID PROTEIN; MARGATOXIN; POTASSIUM CHANNEL BLOCKING AGENT; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOPLASM; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; FIBROBLAST; HUMAN; HUMAN CELL; IC 50; LYMPHOCYTE PROLIFERATION; MASS SPECTROMETRY; MOUSE; MUTANT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN REFOLDING; SYNTHESIS; T LYMPHOCYTE; WESTERN BLOTTING;

ANIMALS; CELL LINE; CELL PROLIFERATION; FIBROBLASTS; MICE; PICHIA; POTASSIUM CHANNEL BLOCKERS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SCORPION VENOMS; TOXINS, BIOLOGICAL;

PICHIA; PICHIA PASTORIS; SCORPIONES;

EID: 84871548782     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0052965     Document Type: Article

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