A designed bifunctional laccase/β-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse

Protein Engineering, Design and Selection

Volumn 26, Issue 1, 2013, Pages 15-23

  Furtado, G P ^a   Ribeiro, L F ^a   Lourenzoni, M R ^a,b,c   Ward, R J ^a,d  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b Verdartis Desenvolvimento Biotecnologico Ltda ME   (Brazil)

^c INSTITUTO OSWALDO CRUZ   (Brazil)

^d BRAZILIAN CENTER FOR RESEARCH IN ENERGY AND MATERIALS CNPEM   (Brazil)

Author keywords

Bacillus subtilis; chimeric enzyme; gene fusion; rational design

Indexed keywords

BACILLUS SUBTILIS; BIFUNCTIONAL; BIFUNCTIONAL ENZYMES; CATALYTIC DOMAINS; CATALYTIC FUNCTIONS; CHIMERIC ENZYMES; EQUIMOLAR MIXTURES; GENE FUSION; GLUCANASE; GLUCANS; HYDROLYTIC ACTIVITIES; INTER-DOMAIN; LACCASE ACTIVITY; LACCASES; MOLECULAR DYNAMICS SIMULATIONS; PLANT CELL WALL; RATIONAL DESIGN; SIMULTANEOUS REDUCTION; SUGAR-CANE BAGASSE;

BAGASSE; CATALYSIS; ESCHERICHIA COLI; MOLECULAR DYNAMICS; PLANT CELL CULTURE;

ENZYME ACTIVITY;

BAGASSE; BETA 1,3 1,4 GLUCANASE; GLUCAN GLUCOSIDASE; LACCASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CATALYSIS; CHIMERA; CIRCULAR DICHROISM; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; GENE INSERTION; HYDROLYSIS; MOLECULAR DYNAMICS; NONHUMAN; PH; PRIORITY JOURNAL; SUGARCANE;

BACILLUS SUBTILIS; CELLULOSE; ENDO-1,3(4)-BETA-GLUCANASE; KINETICS; LACCASE; MOLECULAR DYNAMICS SIMULATION; OXIDATION-REDUCTION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SACCHARUM;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 84871229274     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs057     Document Type: Article

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