Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance

Journal of Biological Chemistry

Volumn 286, Issue 50, 2011, Pages 43026-43038

  Ribeiro, Lucas F ^a   Furtado, Gilvan P ^a   Lourenzoni, Marcos R ^a,b   Costa Filho, Antonio J ^a   Santos, Camila R ^c   Peixoto Nogueira, Simone C ^a   Betini, Jorge A ^a   Polizeli, Maria De Lourdes T M ^a   Murakami, Mario T ^c   Ward, Richard J ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b Verdartis Desenvolvimento Biotecnologico Ltda ME   (Brazil)

^c Centro Nacional de Pesquisa em Energia e Materiais   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; BACILLUS SUBTILIS; BIFUNCTIONAL; BIFUNCTIONAL ENZYMES; BIOPHYSICAL METHODS; CATALYTIC DOMAINS; CATALYTIC EFFICIENCIES; CATALYTIC PERFORMANCE; CATALYTIC TURNOVER; CHIMERIC ENZYMES; CODING SEQUENCES; EPR SPECTROSCOPY; GENE FUSION; IN-VITRO; INTER-DOMAIN; KINETIC MEASUREMENT; LACCASE ACTIVITY; LACCASES; LOW RESOLUTION; MOLECULAR DYNAMICS SIMULATIONS; MOLECULAR EVOLUTION; SMALL ANGLE X-RAY SCATTERING; STRUCTURAL CHANGE; SURFACE LOOPS; TWO DOMAINS; UV-VISIBLE; WILD TYPES; XYLANASES;

BACTERIOLOGY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYMES; GENES; MOLECULAR BIOLOGY;

MOLECULAR DYNAMICS;

COPPER ION; ENZYME VARIANT; LACCASE; LEUCINE; LYSINE; SOLVENT; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BIOPHYSICS; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; COTA GENE; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CHEMISTRY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME METABOLISM; GENE FUSION; GENE INSERTION; MOLECULAR DYNAMICS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; TEMPERATURE SENSITIVITY; THERMOSTABILITY; ULTRAVIOLET SPECTROSCOPY; WILD TYPE; X RAY CRYSTALLOGRAPHY; XYNA GENE;

ELECTRON SPIN RESONANCE SPECTROSCOPY; HYDROGEN-ION CONCENTRATION; KINETICS; LACCASE; MOLECULAR DYNAMICS SIMULATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; XYLOSIDASES;

EID: 83355169737     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.253419     Document Type: Article

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