Assembling a novel bifunctional cellulase-xylanase from Thermotoga maritima by end-to-end fusion

Biotechnology Letters

Volumn 28, Issue 22, 2006, Pages 1857-1862

  Hong, Su Young ^a   Lee, Jin Suk ^a   Cho, Kye Man ^a   Math, Renukaradhya K ^a   Kim, Yong Hee ^a   Hong, Sun Joo ^a   Cho, Yong Un ^b   Kim, Hoon ^c   Yun, Han Dae ^a  

^a Gyeongsang National University   (South Korea)

^b Chinju National University   (South Korea)

^c Sunchon National University   (South Korea)

Author keywords

Bifunctional enzyme; Cellulase; Gene fusion; Xylanase

Indexed keywords

BIFUNCTIONAL ENZYMES; CELLULASE; GENE FUSION; XYLANASE;

ENZYME KINETICS; FUSION REACTIONS; MOLECULAR DYNAMICS; PROTEINS;

CELLULOSE;

CELLULASE; CELLULOSE; DNA; ENZYME; HYBRID PROTEIN; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; BIOTECHNOLOGY; CHEMISTRY; HYDROLYSIS; METABOLISM; METHODOLOGY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE; THERMOTOGA MARITIMA;

BIOTECHNOLOGY; CELLULASE; CELLULOSE; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYMES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; RECOMBINANT FUSION PROTEINS; TEMPERATURE; THERMOTOGA MARITIMA; XYLOSIDASES;

ESCHERICHIA COLI; THERMOTOGA MARITIMA;

EID: 33749643534     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9166-8     Document Type: Article

References (17)

1. An JM, Kim YK, Lim WJ, Hong SY, An CL, Shin EC, Cho KM, Choi BR, Kang JM, Lee SM, Kim H, Yun HD (2005) Evaluation of a novel bifunctional xylanase-cellulase constructed by gene fusion. Enzyme Microb Technol 36:989-995
2. Bergquist PL, Gibbs MD, Morris DD, Thompson DR, Uhl AM, Daniel RM (2001) Hyperthermophilic xylanases. Methods Enzymol 330:301-319
3. Bok JD, Yernool DA, Eveleigh DE (1998) Purification, characterization, and molecular analysis of thermostable cellulases CelA and CelB from Thermotoga neapolitana. Appl Environ Microbiol 64:4774-4781
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
5. Bronnenmeier K, Kern A, Liebl W, Staudenbauer WL (1995) Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials. Appl Environ Microbiol 61:1399-1407
6. Chhabra SR, Kelly RM (2002) Biochemical characterization of Thermotoga maritima endoglucanase Cel74 with and without a carbohydrate binding module (CBM). FEBS Lett 531:375-380
7. Chhabra SR, Shockley KR, Conners SB, Scott KL, Wolfinger RD, Kelly RM (2003) Carbohydrate-induced differential gene expression patterns in the hyperthermophilic bacterium Thermotoga maritima. J Biol Chem 278:7540-7552
8. Chhabra SR, Shockley KR, Ward DE, Kelly RM (2002) Regulation of endo-acting glycosyl hydrolases in the hyperthermophilic bacterium Thermotoga maritima grown on glucan-and mannan-based polysaccharides. Appl Environ Microbiol 68:545-554
9. Doi N, Yanagawa H (1999) Insertional gene fusion technology. FEBS Lett 457:1-4
10. Gilbert M, Bayer R, Cunningham AM, DeFrees S, Gao Y, Watson DC, Young NM, Wakarchuk WW (1998) The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion. Nat Biotechnol 16:769-772
11. Goyal K, Selvakumar P, Hayashi K (2001) Characterization of a thermostable β-glucosidase (BglB) from Thermotoga maritima showing transglycosylation activity. J Mol Cata B: Enzymatic 15:45-53
12. Jiang Z, Zhu Y, Li L, Yu X, Kusakabe I, Kitaoka M, Hayashi K (2004) Transglycosylation reaction of xylanase B from the hyperthermophilic Thermotoga maritima with the ability of synthesis of tertiary alkyl β-D-xylobiosides and xylosides. J Biotechnol 114:125-134
13. Lu Q (2005) Seamless cloning and gene fusion. Trends Biotechnol 23:199-207
14. Nixon AE, Ostermeier M, Benkovic SJ (1998) Hybrid enzymes: manipulating enzyme design. Trends Biotechnol 16:258-264
15. Park SR, Lim WJ, Kim MK, Hong SY, Shin EC, Kim EJ, Lee JR, Woo JG, Kim H, Yun HD (2004) Analysis of cel and pel genes from Pectobacterium chrysanthemi PY35 for relatedness to pathogenecity. J Microbiol Biotechnol 14:1047-1051
16. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
17. Wassenberg D, Liebl W, Jaenicke R (2000) Maltose-binding protein from the hyperthermophilic bacterium Thermotoga maritima: stability and binding properties. J Mol Biol 295:279-288