Validation of the use of multiple internal control genes, and the application of real-time quantitative PCR, to study esterase gene expression in Oenococcus oeni

Applied Microbiology and Biotechnology

Volumn 96, Issue 4, 2012, Pages 1039-1047

  Sumby, Krista M ^a   Grbin, Paul R ^a   Jiranek, Vladimir ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Esterase; Oenococcus oeni; QPCR; Reference genes

Indexed keywords

ESTER SYNTHESIS; ESTERASE; EXPRESSION LEVELS; IDENTIFICATION AND EVALUATION; INDEPENDENT SET; INTERNAL CONTROLS; MULTIPLE REFERENCES; NORMALISATION; OENOCOCCUS OENI; QPCR; REAL-TIME PCR; REAL-TIME QUANTITATIVE PCR; TARGET GENE EXPRESSION; TARGET GENES;

BACILLI; ENZYMES; ESTERS; POLYMERASE CHAIN REACTION;

GENE EXPRESSION;

BACTERIAL DNA; ESTERASE;

BACTERIUM; ENZYME ACTIVITY; ESTER; GENE EXPRESSION; GENETIC ENGINEERING; HYDROLYSIS; POLYMERASE CHAIN REACTION; SUBSTRATE;

ARTICLE; BACTERIAL STRAIN; DNA EXTRACTION; ENZYME SYNTHESIS; GENE EXPRESSION; GENE IDENTIFICATION; HYDROLYSIS; NONHUMAN; OENOCOCCUS OENI; REAL TIME POLYMERASE CHAIN REACTION; VALIDATION PROCESS;

BACTERIAL PROTEINS; ESTERASES; GENE EXPRESSION PROFILING; OENOCOCCUS; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS;

OENOCOCCUS OENI;

EID: 84870992729     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4409-1     Document Type: Article

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