A thermostable variant of P. Aeruginosa cold-adapted LipC obtained by rational design and saturation mutagenesis

Process Biochemistry

Volumn 47, Issue 12, 2012, Pages 2064-2071

  Cesarini, Silvia ^a,b   Bofill, Cristina ^a,b   Pastor, F I Javier ^a   Reetz, Manfred T ^b,c   Diaz, Pilar ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^c PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Cold adapted lipase; Enzyme improvement; Pseudomonas; Rational design; Saturation mutagenesis

Indexed keywords

COLD-ADAPTED LIPASE; ENVIRONMENTAL APPLICATIONS; ENZYME STRUCTURES; HIGH CONCENTRATION; LOW TEMPERATURES; MUTANT CLONES; MUTANT LIBRARIES; OPERATIONAL PROCESS; P.AERUGINOSA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; RATIONAL DESIGN; SATURATION MUTAGENESIS; SPECIFIC SITES; SUBSTRATE SPECIFICITY; TWO-POINT; WILD TYPES;

AMINO ACIDS; BACTERIA; ENZYMES; HEAVY METALS; MUTAGENESIS; THERMODYNAMIC STABILITY;

DESIGN;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 84870829956     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2012.07.023     Document Type: Article

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