Detection of klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)

International Food Research Journal

Volumn 19, Issue 4, 2012, Pages 1757-1762

  Puspanadan, S ^a   Afsah Hejri, L ^a   Loo, Y Y ^a   Nillian, E ^a   Kuan, C H ^a   Goh, S G ^a   Chang, W S ^a   Lye, Y L ^a   John, Y H T ^b   Rukayadi, Y ^a   Yoshitsugu, N ^c   Nishibuchi, M ^c   Son, R ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UNIVERSITI SULTAN ZAINAL ABIDIN   (Malaysia)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

(MPN); (PCR); Klebsiella pneumoniae; Most probable number; Polymerase chain reaction; Raw vegetables

Indexed keywords

CUCUMIS SATIVUS; DAUCUS CAROTA; KLEBSIELLA; KLEBSIELLA PNEUMONIAE; LACTUCA; LYCOPERSICON ESCULENTUM; PETROSELINUM CRISPUM;

EID: 84870502249     PISSN: 19854668     EISSN: 22317546     Source Type: Journal    
DOI: None     Document Type: Article

References (64)

1. Adams, M.R., Hartley, A.D. and Cox, L.J. 1989. Factors affecting the efficacy of washing procedures used in the production of prepared salads. Food Microbiology 6: 69-77.
2. Albrecht, J.A., Hamouz, F.L., Sumner, S.S. and Melch, V. 1995. Microbial evaluation of vegetable ingredients in salad bars. Journal of Food Protection 58: 683-685.
3. Albrecht, J.A., Hamouz, F.L., Sumner, S.S., and Melch, V. 1995. Microbial evaluation of vegetable ingredients in salad bars. Journal of Food Protection 58: 683-685.
4. Bennik, M.H.J., Vorstman, W., Smid, E.J. and Gorris, L.G.M. 1998. The influence of oxygen and carbon dioxide on the growth of the prevalent Enterobacteriaceae and Pseudomonas species isolated from fresh and controlled-atmosphere-stored vegetables. Food Microbiology 15: 459-469.
5. Beuchat, L.R. 2002. Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbiology Infection 4: 413-423.
6. Beuchat, L.R. 1995. Pathogenic micro organisms associated with fresh produce. Journal of Food Protection 59:204-216.
7. Beuchat, L.R., Nail, B.V., Adler, B.B. and Clavero, M.R.S. 1998. Efficacy of spray application of chlorinated water in killing pathogenic bacteria on raw apples, tomatoes, and lettuce. Journal of Food Protection 61:1305-1311.
8. Blackman, I.C. and Frank, J.F. 1996. Growth of Listeria monocytogenes as a biofilm on various food processing surfaces. Journal of Food Protection 59: 827-831.
9. Brackett, R.E. 1992. Shelf stability and safety of fresh produce as influenced by sanitation and disinfection. Journal of Food Protection 55: 808-814.
10. Brandl, M. 2006. Fitness of human enteric pathogens on lants and implications for food safety. Annual Review of Phytopathology 44: 53-63.
11. Brocklehurst, T.F., Zaman-Wong, C.M. and Lund, B.M. 1987. A note on the microbiology of retail packs of prepared salad vegetables. Journal of Applied Bacteriology 63: 409-415.
12. Bush, K. and Singer, S.B. 1989. Biochemical characteristics of extended broad spectrum β-lactamases. Infection 17: 429-433.
13. Bush, K., Jacoby, G.A. and Medeiros, A.A. 1995. A functional classi?cation scheme for β-lactamases and its correlation with molecular structure. Antimicrobial Agents and Chemotherapy 39: 1211-1233.
14. De Roever, C. 1998. Microbiological safety evaluations and recommendations on fresh produce. Food Control 6: 321-347.
15. Doyle, J.J. and Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of tissue. Phytochemical Bullletin 19: 11-15.
16. Duncan, D.W. and Razzell, W.E. 1972. Klebsiella biotypes among coliforms isolated from forest environments and farm produce. Applied Microbiol 24:933-938.
17. Ercolani, G.L. 1976. Bacteriological quality assessment of fresh marketed lettuce and fennel. Applied and Environmental Microbiology 31: 847-852.
18. Francis, G.A., Thomas, C. and O'Beirne, D. 1999. The microbiologcal safety of minimally processed vegetables. International Journal of Food Science and Technology 34: 1-22.
19. Garg, N., Churey, J.J. and Splittstoesser, D.F. 1990. Effect of processing conditions on the microflora of fresh-cut vegetables. Journal of Food Protection 53:701-703.
20. Geldreich, E.E., Huf, C.B., Bordner, R.H., Kabler, P.W. and Clark, H.F. 1962. The faecal coli-aerogenes flora of soils from various geographical areas. Journal of Applied Bacteriology 25: 87-93.
21. Ghenghesh, K. S., Belhaj, K., El-Amin, W. B., El-Nefathi, S. E. and Zalmum, A. 2004. Microbiological quality of fruit juices sold in Tripoli-Libya. Food Control 16(10):855-858.
22. Gourama, H., Tsai, W.Y.J. and Bullerman, L.B. 1991. Growth and production of enterotoxins A and D by Staphylococcus aureus in salad bar ingredients and clam chowder. Journal of Food Protection 54:844-847.
23. Gupta, N., Khan, D. K. and Santra, S. C. 2009. Prevalence of intestinal helminth eggs on vegetables grown in wastewater-irrigated areas of Titagarh, West Bengal, India. Food Control 20: 942-945.
24. Hamilton, A. Stagnitti, F. Premier, R. Boland, A.M. and Hale, G. 2006. Quantitative microbial risk assessment models for consumption of raw vegetables irrigated with reclaimed water. Applied and Environmental Microbiology. 72, 3284-3290.
25. Jacoby, G. A. and Medeiros, A.A. 1991. More extendedspectrum β-lactamases. Antimicrobial Agents and Chemotherapy 35: 1697-1704.
26. Jarlier, V., Nicolas, M., Fournier, G. and Philippon, A. 1988. Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Reviews of Infection Disease 10: 867-878.
27. Johannessen, G.S., Loncarevic, S. and Kruse, H. 2002. Bacteriological analysis of fresh produce in Norway. International Journal of Food Microbiology 77: 199-204.
28. King, Jr. A.D., Magnuson, J.A., Torok, T. and Goodman, N. 1991. Microbial flora and storage quality of partially processed lettuce. Journal of Food Science 56: 459-461.
29. Leben, C. 1988. Relative humidity and the survival of epiphytic bacteria on buds and leaves of cucumber plants. Phytopathology 78: 179-185.
30. Leigh, J.A. and Coplin, D.L. 1992. Exopolysaccarides in plant-bacterial interaction. Annual Reviews Microbiology 46:307-346.
31. Lisle, J.T., Broadaway, S.C., Prescott, A.M., Pyle, B.H., Fricker, C. and McFeters, G.A. 1998. Effects of starvation on physiological activity and chlorine disinfection resistance in Escherichia coli O157:H7. Applied Environmental Microbiology 64: 4658-4662.
32. Livermore, D. M. 1995. β-Lactamases in laboratory and clinical resistance. Clinical Microbiology Reviews 8:557-584.
33. Lopes, A. C. D. S., Rodrigues, J. F. and Morais, M. A. D. 2005. Molecular typing of Klebsiella pneumoniae isolates from public hospitals in Recife, Brazil. Microbiological Research 160:37-46.
34. Lund, B.M. 1992. Ecosystems in vegetable foods. Journal of Applied Bacteriology. Sympossium Supplement 73: 115-126.
35. Mabilat, C. and Courvalin, P. 1990. Development of "oligotyping" for characterization and molecular epidemiology of TEM β-lactamases in members of the family Enterobacteriaceae. Antimicrobial Agents and Chemotherapy 34: 2210-2216.
36. Medeiros, A., Mayer, K.H. and Opal, S.M. 1988. Plasmidmediated Betalactamases. Antimicrobic Newsletter 5:61-65.
37. Morris, C.E., Monier, J.E. and Jacques, M.A. 1997 Methods for observing microbial biofilms directly on leaf surfaces and recovering them for isolation of culturable microorganisms, Applied Environmental Microbiology 63: 1570-1576.
38. Mpuchane, S. F. and Gashe, B. A. 1996. Presence of Escherichia coli, Klebsiella pneumoniae and Enterobacter species in dried bush okra (Corchorus olitorius) and African spider herb (Cleome gynandra). Food Control 7(3): 169-172.
39. Nguyen-the, C. and Carlin, F. 1994. The microbiology of minimally processed fresh fruits and vegetables. Critical Reviews in Food Science and Nutrition 34:371-401.
40. Odumeru, J.A., Mitchell, S.J., Alves, D.M., Lynch, J.A., Yee, A.J., Wang, S.L., Styliadis, S. and Farber, J.M. 1997. Assessment of the microbiological quality of ready-to-use vegetables for healthcare food services. Journal of Food Protection 60: 954-960.
41. Ouellette, M., Paul, G.S., Philippon, A.M. and Roy, P.H. 1988. Oligonucleotide probes (TEM-1, OXA-1) versus isoelectric focusing in β-lactamase characterization of 114 resistant strains. Antimicrobial Agents and Chemotherapy 32: 397-399.
42. Pangon, B., Bizet, C., Bure, C., Pichon, F., Philippon, A., Regnier, B. and Gutmann, L. 1989. In vivo selection of a cephamycin-resistant, porin-de?cient mutant of Klebsiella pneumoniae producing a TEM-3 β -lactamase. Journal of Infection Disease 159: 1005-1006.
43. Podschun, R. and Ullmann, U. 1998. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical Microbiology Reviews 11(4): 589-603.
44. Ponniah, J., Tunung, R., Margaret, S.P., Son, R., Farinazleen, M.G., Cheah, Y.K., Nishibuchi, M., Nakaguchi, Y. and Malakar, P.K. 2010. Listeria monocytogenes in raw salad vegetables sold at retail level in Malaysia. Food Control 21: 774-778.
45. Rao, D.V. and Rao, K.R.G. 1983. Some characteristics of Klebsiella strains isolated from foods and water. Journal of Food Science and Technology 20: 269-272.
46. Reynolds, G., Mekras, C., Perry, R. and Graham, N. 1989. Alternative disinfectant chemicals for trihalomethane-a review. Environmental Technology (Letters) 10: 591-595.
47. Richmond, M. H. and Sykes, R.B. 1973. The β-lactamases of gram negative bacteria and their possible physiological role. Advances in Microbial Physiology 9: 31-88.
48. Robbs, P.G., Bartz, J.A., Sargent, S.A., McFie, G. and Hodge, N.C. 1996. Potential inoculum sources for decay of fresh-cut celery. Journal of Food Science 61:449-452.
49. Ryan, K.J. and Ray, C.G. 2004. Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0-8385-8529-9.
50. Sabota, J. M., Hoppes, W. L., Ziegler, J. R., DuPont, H., Mathewson, J. and Rutecki, G. W. 1998. A new variant of food poisoning: Enteroinvasive Klebsiella pneumoniae and Escherichia coli sepsis from a contaminated hamburger. The American Journal of Gastroenterology 93(1): 118-119.
51. Sahilah, A.M., Tuan Suraya, T. S., Noraida., I., Ahmad Azuhairi, A., Chai, L. C. and Son, R. 2010. Detection of virulence genes and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis among raw vegetables isolates of Campylobacter jejuni. International Food Research Journal 17: 681-690.
52. Shahid, M. Malik, A. Adil, M. Jahan, N. and Malik, R. 2009. Comparison of beta-lactamases genes in clinical bacterial isolates in India. Journal of Infection in Developing Countries 3(8): 593-598.
53. Soriano, J.M. Rico, H. Molto, J.C. and Manes, J. 2000. Assessment of the microbiological quality and wash treatments of lettuce served in University restaurants. International Journal of Food Microbiology 58: 123-128.
54. Splittstoesser DF, Queale DT, Bowers JL, Wilkson M.1980. Coliform content of frozen blanched vegetables packed in the United States. Journal of Food Safety 2:1-11.
55. Thomson, K. S., Sanders, C.C. and Moland, E.S. 1999. Use of microdilution panels with and without β-lactamase inhibitors as a phenotypic test for β-lactamase production among Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter freundii, and Serratia marcescens. Antimicrobial Agents and Chemotherapy 43: 1393-1400.
56. Tsukadaira, A., Okubo, Y., Kobayashi, T., Wakamatsu, T., Sasabayashi, M., and Hotta, J. 2002. Four cases of Klebsiella pneumonia. Nihon Kokyuki Gakkai Zasshi 40(6): 530-535.
57. Tunung, R., Margaret, S.P., Jeyaletchumi, P., Chai, L.C., Zainazor, T.C., Ghazali, F.M., Nakaghuchi, Y., Nishibuchi, M. and Son, R. 2010. Prevalence and quantification of Vibrio in raw vegetables at retail level. Journal of Microbiology and Biotechnology 20(2): 391-396.
58. Tunung, R., Ghazali, F.M., Noranizan, M.A., Haresh, K.K., Lesley, M.B., Nakaguchi, Y., Nishibuchi, M. and Son, R. 2011. Rapid detection and enumeration of pathogenic Vibrio parahaemolyticus in raw vegetables from retail outlets. International Food Research Journal 18: 67-78.
59. Usha, M. R., Tunung, R., Chai, L. C., Ghazali, F. M., Cheah, Y. K., Nishibuchi, M. and Son, R. 2010. A study on Campylobacter jejuni cross-contamination during chilled broiler preparation. International Food Research Journal 17: 107-115.
60. Van Duyn, M.A.S. and Pivonka, E. 2000. Overview of the health benefits of fruit and vegetable consumption for the dietetics professional: selected literature. Journal of the American Dietetic Association 100: 1511-1521.
61. Van Gerwen, S.J.C., de Wit, J.C., Notermans, S. and Zwietering, M.H. 1997. An identification procedure for foodborne microbial hazards. International Journal of Food Microbiology 38: 1-15.
62. Willocx, F., Tobback, P. and Hendrickx, M. 1994. Microbial safety assurance of minimally processed vegetables by implementation of the hazard analysis critical control point (HACCP) system. Acta Alimentaria 23:221-238.
63. Yang, Z., Jiao, X., Cao, G., Fang, W. and Gu, R. 2008. Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried and salted seafood products in two coastal areas of eastern China. International Journal of Food Microbiology 125: 279-285.
64. Zhao, T., Clavero, M.R.S., Doyle, M.P. and Beuchat, L.R. 1997. Health relevance of the presence of fecal coliforms in iced tea and in leaf tea. Journal of Food Protection 60:215-218