Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putida

Journal of Biological Engineering

Volumn 6, Issue , 2012, Pages

  Mukherjee, Arnab ^a   Weyant, Kevin B ^a   Walker, Joshua ^a   Schroeder, Charles M ^a,b,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c University of Illinois at Urbana Champaign   (United States)

Author keywords

Directed evolution; Flavin binding fluorescent proteins; Site saturation mutagenesis

Indexed keywords

AMINO ACID; AROMATIC AMINO ACID; BACTERIAL PROTEIN; BINDING PROTEIN; FLAVIN BINDING FLUORESCENT PROTEIN; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; HYDROXYL GROUP; MUTANT PROTEIN; OLIGONUCLEOTIDE; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARTICLE; BACTERIAL CHROMATOPHORE; CELL TRANSFORMATION; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA SEQUENCE; ESCHERICHIA COLI; MUTAGENESIS; MUTATION RATE; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PSEUDOMONAS PUTIDA; QUANTUM YIELD; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; SITE SATURATION MUTAGENESIS; SPECTROFLUOROMETRY;

BACILLUS SUBTILIS; PSEUDOMONAS PUTIDA;

EID: 84867666487     PISSN: None     EISSN: 17541611     Source Type: Journal    
DOI: 10.1186/1754-1611-6-20     Document Type: Article

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