Modulation of inflammatory gene expression by a bilberry (Vaccinium myrtillus L.) extract and single anthocyanins considering their limited stability under cell culture conditions

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 36, 2012, Pages 8902-8910

  Triebel, Sven ^a   Trieu, Hai Linh ^a   Richling, Elke ^a  

^a Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

anthocyanins; bilberry (Vaccinium myrtillus L.) extract; cytokines; inflammatory bowel disease (IBD); inflammatory gene expression; stability

Indexed keywords

ANTHOCYANIDINS; ANTI-INFLAMMATORIES; ANTI-INFLAMMATORY ACTIVITY; CELL CULTURE MEDIUMS; CULTURE CONDITIONS; CYTOKINES; DEGRADATION PRODUCTS; EPITHELIAL CELLS; HUMAN COLON; INFLAMMATORY BOWEL DISEASE; INFLAMMATORY GENES; INHIBITORY EFFECT; PARENT COMPOUNDS; Q-RT-PCR; STIMULATED CELLS; SUGAR MOIETY; VACCINIUM MYRTILLUS;

ALKYLATION; CELL CULTURE; CONVERGENCE OF NUMERICAL METHODS; DEGRADATION; GENE EXPRESSION; STABILITY;

ANTHOCYANINS;

ANTHOCYANIN; ANTIINFLAMMATORY AGENT; CYTOKINE; PLANT EXTRACT;

CHEMISTRY; CONFERENCE PAPER; CULTURE TECHNIQUE; DRUG EFFECT; DRUG STABILITY; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOLOGY; INFLAMMATION; VACCINIUM MYRTILLUS;

ANTHOCYANINS; ANTI-INFLAMMATORY AGENTS; CELL CULTURE TECHNIQUES; CYTOKINES; DRUG STABILITY; GENE EXPRESSION; HUMANS; INFLAMMATION; PLANT EXTRACTS; VACCINIUM MYRTILLUS;

VACCINIUM MYRTILLUS;

EID: 84866429142     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf3028842     Document Type: Conference Paper

References (34)

1. Prior, R. L.; Wu, X. Anthocyanins: structural characteristics that result in unique metabolic patterns and biological activities Free Radical Res. 2006, 40, 1014-1028
2. Clifford, M. N. Anthocyanins - nature, occurrence and dietary burden J. Sci. Food Agric. 2000, 80, 1063-1072
3. Mazza, G.; Velioglu, Y. S. Anthocyanins and other phenolic compounds in fruits of red-fleshed apples Food Chem. 1992, 43, 113-117
4. Wu, X.; Beecher, G. R.; Holden, J. M.; Haytowitz, D. B.; Gebhardt, S. E.; Prior, R. L. Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption J. Agric. Food Chem. 2006, 54, 4069-4075
5. Ovaskainen, M. L.; Törrönen, R.; Koponen, J. M.; Sinkko, H.; Hellström, J.; Reinivuo, H.; Mattila, P. Dietary intake and major food sources of polyphenols in Finnish adults J. Nutr. 2008, 138, 562-566
6. Koponen, J. M.; Happonen, A. M.; Mattila, P. H.; Törrönen, A. R. Contents of anthocyanins and ellagitannins in selected foods consumed in Finland J. Agric. Food Chem. 2007, 55, 1612-1619
7. Kern, M.; Fridrich, D.; Reichert, J.; Skrbek, S.; Nussher, A.; Hofem, S.; Vatter, S.; Pahlke, G.; Rüfer, C.; Marko, D. Limited stability in cell culture medium and hydrogen peroxide formation affect the growth inhibitory properties of delphinidin and its degradation product gallic acid Mol. Nutr. Food Res. 2007, 51, 1163-1172
8. Woodward, G.; Kroon, P.; Cassidy, A.; Kay, C. Anthocyanin stability and recovery: Implications for the analysis of experimental and clinical samples J. Agric. Food Chem. 2009, 57, 5271-5278
9. Belitz, H. D.; Grosch, W.; Schieberle, P. Fruits and fruit products, anthocyanidins. In Food Chemistry, 4 th ed.; Springer-Verlag: Berlin, Germany, 2009; pp 829-831.
10. Schantz, M.; Mohn, C.; Baum, M.; Richling, E. Antioxidative efficiency of an anthocyanin rich bilberry extract in the human colon tumor cell lines Caco-2 and HT-29 J. Berry Res. 2010, 1, 25-33
11. Kong, J. M.; Chia, L. S.; Goh, N. K.; Chia, T. F.; Brouillard, R. Analysis and biological activities of anthocyanins Phytochemistry 2003, 64, 923-933
12. Scalbert, A.; Manach, C.; Morand, C.; Rémésy, C.; Jiménez, L. Dietary polyphenols and the prevention of diseases Crit. Rev. Food Sci. Nutr. 2005, 45, 287-306
13. Chen, J.; Uto, T.; Tanigawa, S.; Kumamoto, T.; Fujii, M.; Hou, D. X. Expression profiling of genes targeted by bilberry (Vaccinium myrtillus) in macrophages through DNA microarray Nutr. Cancer 2008, 60, 43-50
14. Hou, D. X.; Yanagita, T.; Uto, T.; Masuzaki, S.; Fujii, M. Anthocyanidins inhibit cyclooxygenase-2 expression in LPS-evoked macrophages: structure-activity relationship and molecular mechanisms involved Biochem. Pharmacol. 2005, 70, 417-425
15. Mauray, A.; Felgines, C.; Morand, C.; Mazur, A.; Scalbert, A.; Milenkovic, D. Bilberry anthocyanin-rich extract alters expression of genes related to atherosclerosis development in aorta of apo E-deficient mice Nutr. Metab. Cardiovasc. Dis. 2012, 22, 72-80
16. Min, S. W.; Ryu, S. N.; Kim, D. H. Anti-inflammatory effects of black rice, cyanidin-3- O -β- d -glycoside, and its metabolites, cyanidin and protocatechuic acid Int. Immunopharmacol. 2010, 10, 959-966
17. Bouma, G.; Strober, W. The immunological and genetic basis of inflammatory bowel disease Nat. Rev. Immunol. 2003, 3, 521-533
18. Pitman, R. S.; Blumberg, R. S. First line of defence: the role of the intestinal epithelium as an active component of the mucosal immune system J. Gastroenterol. 2000, 35, 805-814
19. Eckmann, L.; Jung, H. C.; Schurer-Maly, C.; Panja, A.; Morzycka-Wroblewska, E.; Kagnoff, M. F. Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin 8 Gastroenterology 1993, 105, 1689-1697
20. Banks, C.; Bateman, A.; Payne, R.; Johnson, P.; Sheron, N. Chemokine expression in IBD. Mucosal chemokine expression is unselectively increased in both ulcerative colitis and Crohn's disease J. Pathol. 2003, 199, 28-35
21. Yang, S. K.; Eckmann, L.; Panja, A.; Kagnoff, M. F. Differential and regulated expression of C-X-C, C-C, and C-chemokines by human colon epithelial cells Gastroenterology 1997, 113, 1214-1223
22. O'Brien, J.; Wilson, I.; Orton, T.; Pognan, F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity Eur. J. Biochem. 2000, 267, 5421-5426
23. Pfaffl, M. W. A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res. 2001, 29, 2002-2007
24. Porath, D.; Riegger, C.; Drewe, J.; Schwager, J. EGCG impairs chemokine production in human colon epithelial cell lines J. Pharmacol. Exp. Ther. 2005, 315, 1172-1180
25. Potoka, D. A.; Nadler, E. P.; Zhou, X.; Zhang, X. R.; Upperman, J. S.; Ford, H. R. Inhibition of NF-κB by IκB prevents cytokine-induced NO production and promotes enterocyte apoptosis in vitro Shock 2000, 14, 366-373
26. Andoh, A.; Yagi, Y.; Shioya, M.; Nishida, A.; Tsujikawa, T.; Fujiyama, Y. Mucosal cytokine network in inflammatory bowel disease World J. Gastroenterol. 2008, 14, 5154-5161
27. Singh, U. P.; Venkataraman, C.; Singh, R.; Lillard, J. CXCR3 axis: role in inflammatory bowel disease and its therapeutic implication Endocr. Metab. Immune Disord. Drug Targets 2007, 7, 111-123
28. Dwinell, M. B.; Lugering, N.; Eckmann, L.; Kagnoff, M. F. Regulated production of interferon-inducible T-cell chemoattractants by human intestinal epithelial cells Gastroenterology 2001, 120, 49-59
29. Alzoghaibi, M. A.; Al-Mofleh, I. A.; Al-Jebreen, A. M. Neutrophil chemokines GCP-2 and GRO-α in patients with inflammatory bowel disease J. Dig. Dis. 2008, 9, 144-148
30. Song, W. B.; Lv, Y. H.; Zhang, Z. S.; Li, Y. N.; Xiao, L. P.; Yu, X. P.; Wang, Y. Y.; Ji, H. L.; Ma, L. Soluble intercellular adhesion molecule-1, d -lactate and diamine oxidase in patients with inflammatory bowel disease World J. Gastroenterol. 2009, 15, 3916-3919
31. Kahle, K.; Kraus, M.; Scheppach, W.; Ackermann, M.; Ridder, F.; Richling, E. Studies on apple and blueberry fruit constituents: do the polyphenols reach the colon after ingestion? Mol. Nutr. Food Res. 2006, 50, 418-423
32. Hidalgo, M.; Oruna-Concha, M. J.; Kolida, S.; Walton, G. E.; Kallithraka, S.; Spencer, J. P.; de Pascual-Teresa, S. Metabolism of anthocyanins by human gut microflora and their influence on gut bacterial growth J. Agric. Food Chem. 2012, 60, 3882-3890
33. Forester, S. C.; Waterhouse, A. L. Gut metabolites of anthocyanins, gallic acid, 3- O -methylgallic acid, and 2,4,6-trihydroxybenzaldehyde, inhibit cell proliferation of Caco-2 cells J. Agric. Food Chem. 2010, 58, 5320-5327
34. Jackman, R. L.; Yada, R. Y.; Tung, M. A.; Speers, R. A. Anthocyanins as food colorants J. Food Biochem. 1987, 11, 201-247