Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity

European Journal of Biochemistry

Volumn 267, Issue 17, 2000, Pages 5421-5426

  O'Brien, John ^a   Wilson, Ian ^a   Orton, Terry ^a   Pognan, François ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

Alamar Blue; Cytotoxicity; Fluorescence; Resazurin; Resorufin

Indexed keywords

FLUORESCENT DYE; RESAZURIN; RESORUFIN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL POPULATION; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; CYTOTOXICITY; ENZYME ACTIVITY; FLUORESCENCE; LIVER CELL; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; RAT; REDUCTION;

ANIMALS; CELL SURVIVAL; CULTURE MEDIA; FLUORESCENT DYES; MAGNETIC RESONANCE SPECTROSCOPY; MALE; MASS SPECTROMETRY; OXAZINES; RATS; RATS, WISTAR; XANTHENES;

EID: 0033856957     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01606.x     Document Type: Article

References (16)

1. Dual-attribute continuous monitoring of cell proliferation/cytotoxicity
2. 3H-thymidine incorporation assay
3. Resazurin reducing time as an indicator of bovine semen capacity
4. Assessing the fertility potential of equine semen samples using the reducible dyes methylene green and resazurin
5. Application and evaluation of the Alamar Blue assay for cell growth and survival of fibroblasts
6. A new fluorimetric assay for cytotoxicity measurements in vitro
7. Assessment of the Alamar Blue assay for cellular growth and viability in vitro
8. 3H]thymidine incorporation assay
9. Assessment of neuronal viability with Alamar Blue in cortical and granule cell cultures
10. Isolation of hepatocytes by collagenase perfusion
11. Induction and repair inhibition of oxidative DNA damage by nickel (II) and cadmium (II) in mammalian cells
12. The correlates and alleged biochemical background of the resazurin reduction test in semen
13. Fluorometric determination of carnitine in serum with immobilized carnitine dehydrogenase and diaphorase
14. NAD(P)H: Quinone oxido-reductasel (DT-diaphorase) expression in normal and tumor tissues
15. Cloning and nucleotide sequence of a cDNA of the human erythrocyte NADPH-flavin reductase
16. Stoichiometry of the NADH-oxidoreductase reaction for dehydrogenase determinations