Enhancing the promiscuous phosphotriesterase activity of a thermostable lactonase (GkaP) for the efficient degradation of organophosphate pesticides

Applied and Environmental Microbiology

Volumn 78, Issue 18, 2012, Pages 6647-6655

  Zhang, Yu ^a,b   An, Jiao ^a,b   Ye, Wei ^a   Yang, Guangyu ^a   Qian, Zhi Gang ^a   Chen, Hai Feng ^a   Cui, Li ^a   Feng, Yan ^a,b  

^a Shanghai Jiao Tong University   (China)

^b JILIN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDOHYDROLASE SUPERFAMILY; AMINO ACID SUBSTITUTION; BIOLOGICAL SOLUTIONS; CATALYTIC EFFICIENCIES; CHLORPYRIFOS; DIVERGENT EVOLUTION; ERROR PRONE PCR; HYDROLYTIC ACTIVITIES; IN-VITRO; LACTONASE; LACTONASE ACTIVITY; MOLECULAR DYNAMICS SIMULATIONS; NEUROTOXIC ORGANOPHOSPHATES; ORGANOPHOSPHATE PESTICIDES; PHOSPHOTRIESTERASES; SATURATION MUTAGENESIS; SUBSTRATE SPECIFICITY; WILD-TYPE ENZYMES;

AMINO ACIDS; BIOREMEDIATION; DEGRADATION; ENZYMES; GENES; LEAD COMPOUNDS; MOLECULAR DYNAMICS; MUTAGENESIS;

PESTICIDES;

AMIDASE; CHLORPYRIFOS; DIMPYLATE; LACTONE; MUTANT PROTEIN; ORGANOPHOSPHATE; PARATHION; PESTICIDE; PHOSPHOTRIESTERASE;

BIOREMEDIATION; ENZYME ACTIVITY; LABORATORY METHOD; MUTAGENICITY; MUTATION; ORGANOPHOSPHATE; POLYMERASE CHAIN REACTION;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL STRUCTURE; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; GEOBACILLUS; KINETICS; METABOLISM; METHODOLOGY; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION;

AMIDOHYDROLASES; AMINO ACID SUBSTITUTION; CHLORPYRIFOS; DIAZINON; DIRECTED MOLECULAR EVOLUTION; GEOBACILLUS; KINETICS; LACTONES; MODELS, MOLECULAR; MUTAGENESIS; MUTANT PROTEINS; ORGANOPHOSPHATES; PARATHION; PESTICIDES; PHOSPHORIC TRIESTER HYDROLASES; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

GEOBACILLUS KAUSTOPHILUS;

EID: 84866156180     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01122-12     Document Type: Article

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