Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily

Journal of Biological Chemistry

Volumn 285, Issue 52, 2010, Pages 40911-40920

  Chow, Jeng Yeong ^a   Xue, Bo ^b   Lee, Kang Hao ^a   Tung, Alvin ^b   Wu, Long ^a   Robinson, Robert C ^b   Yew, Wen Shan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE ARCHITECTURE; AMIDOHYDROLASE SUPERFAMILY; CATALYTIC EFFICIENCIES; CATALYTIC REACTIVITY; CHARGE TRANSFER COMPLEX; CHEMICAL CATALYSIS; DIRECTED EVOLUTION; ENZYME ACTIVE SITES; HIGH CONCENTRATION; HOMOSERINE LACTONES; IN-VITRO; LACTONASE; LACTONASE ACTIVITY; MOLECULAR CIRCUITS; MUTANT ENZYMES; N-ACYLHOMOSERINE LACTONES; PATHOGENIC BACTERIUM; PHOSPHOTRIESTERASES; QUORUM-SENSING; SUBSTRATE RANGE; TWO-POINT; WILD TYPES; WILD-TYPE ENZYMES;

BACTERIA; CATALYSTS; CHARGE TRANSFER; HYDROLYSIS; ION EXCHANGE; MASS TRANSFER; QUENCHING;

ENZYMES;

AMIDASE; CATION; ENZYME; GLUCONOLACTONASE; IRON; MUTANT ENZYME; N ACYLHOMOSERINE LACTONE; PHOSPHOTRIESTERASE; TYROSINE; UNCLASSIFIED DRUG; ZINC;

ARTICLE; BACTERIAL VIRULENCE; BIOLUMINESCENCE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; GEOBACILLUS; GEOBACILLUS KAUSTOPHILUS; HYDROLYSIS; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN FAMILY; QUORUM SENSING; THERMOSTABILITY;

AMIDOHYDROLASES; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; GEOBACILLUS; HOT TEMPERATURE; IRON; LACTONES; MULTIGENE FAMILY; MUTATION, MISSENSE; QUORUM SENSING; ZINC;

GEOBACILLUS KAUSTOPHILUS;

EID: 78650352337     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.177139     Document Type: Article

References (36)

1. Gerlt, J. A., and Raushel, F. M. (2003) Curr. Opin. Chem. Biol. 7, 252-264
2. Seibert, C. M., and Raushel, F. M. (2005) Biochemistry 44, 6383-6391
3. Holm, L., and Sander, C. (1997) Proteins 28, 72-82
4. Pegg, S. C., Brown, S. D., Ojha, S., Seffernick, J., Meng, E. C., Morris, J. H., Chang, P. J., Huang, C. C., Ferrin, T. E., and Babbitt, P. C. (2006) Biochemistry 45, 2545-2555
5. Afriat, L., Roodveldt, C., Manco, G., and Tawfik, D. S. (2006) Biochemistry 45, 13677-13686
6. Chow, J. Y., Wu, L., and Yew, W. S. (2009) Biochemistry 48, 4344-4353
7. Camilli, A., and Bassler, B. L. (2006) Science 311, 1113-1116
8. Dong, Y. H., Wang, L. H., Xu, J. L., Zhang, H. B., Zhang, X. F., and Zhang, L. H. (2001) Nature 411, 813-817
9. Yew, W. S., and Gerlt, J. A. (2002) J. Bacteriol. 184, 302-306
10. Chapman, E., and Wong, C. H. (2002) Bioorg. Med. Chem. 10, 551-555
11. Otwinowski, Z., Minor, W., and Charles W. Carter, Jr. (1997) Methods in Enzymology, pp. 307-326, Academic Press, Inc.
12. McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., and Read, R. J. (2007) J. Appl. Crystallogr. 40, 658-674
13. Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L. W., Kapral, G. J., Grosse-Kunstleve, R. W., McCoy, A. J., Moriarty, N. W., Oeffner, R., Read, R. J., Richardson, D. C., Richardson, J. S., Terwilliger, T. C., and Zwart, P. H. (2010) Acta Crystallogr. D Biol. Crystallogr. 66, 213-221
14. Emsley, P., Lohkamp, B., Scott, W. G., and Cowtan, K. (2010) Acta Crystallogr. D Biol. Crystallogr. 66, 486-501
15. Winn, M. D., Isupov, M. N., and Murshudov, G. N. (2001) Acta Crystallogr. D Biol. Crystallogr. 57, 122-133
16. Davis, I. W., Leaver-Fay, A., Chen, V. B., Block, J. N., Kapral, G. J., Wang, X., Murray, L. W., Arendall, W. B., 3rd, Snoeyink, J., Richardson, J. S., and Richardson, D. C. (2007) Nucleic Acids Res. 35, W375-W383
17. Lindsay, A., and Ahmer, B. M. (2005) J. Bacteriol. 187, 5054-5058
18. Choi, K. H., Gaynor, J. B., White, K. G., Lopez, C., Bosio, C. M., Karkhoff-Schweizer, R. R., and Schweizer, H. P. (2005) Nat. Methods 2, 443-448
19. Choi, K. H., and Schweizer, H. P. (2006) Nat. Protoc. 1, 153-161
20. Guzman, L. M., Belin, D., Carson, M. J., and Beckwith, J. (1995) J. Bacteriol. 177, 4121-4130
21. Xiang, D. F., Kolb, P., Fedorov, A. A., Meier, M. M., Fedorov, L. V., Nguyen, T. T., Sterner, R., Almo, S. C., Shoichet, B. K., and Raushel, F. M. (2009) Biochemistry 48, 2237-2247
22. Hawwa, R., Aikens, J., Turner, R. J., Santarsiero, B. D., and Mesecar, A. D. (2009) Arch. Biochem. Biophys. 488, 109-120
23. Elias, M., Dupuy, J., Merone, L., Mandrich, L., Porzio, E., Moniot, S., Rochu, D., Lecomte, C., Rossi, M., Masson, P., Manco, G., and Chabriere, E. (2008) J. Mol. Biol. 379, 1017-1028
24. Krissinel, E., and Henrick, K. (2007) J. Mol. Biol. 372, 774-797
25. Gerlt, J. A., and Babbitt, P. C. (2009) Curr. Opin. Chem. Biol. 13, 10-18
26. Tawfik, D. S. (2006) Science 311, 475-476
27. Vick, J. E., Schmidt, D. M., and Gerlt, J. A. (2005) Biochemistry 44, 11722-11729
28. Davis, J. C., and Averill, B. A. (1982) Proc. Natl. Acad. Sci. U.S.A. 79, 4623-4627
29. Pyrz, J. W., Sage, J. T., Debrunner, P. G., and Que, L., Jr. (1986) J. Biol. Chem. 261, 11015-11020
30. Thoden, J. B., Phillips, G. N., Jr., Neal, T. M., Raushel, F. M., and Holden, H. M. (2001) Biochemistry 40, 6989-6997
31. Martí-Arbona, R., Fresquet, V., Thoden, J. B., Davis, M. L., Holden, H. M., and Raushel, F. M. (2005) Biochemistry 44, 7115-7124
32. Wise, E. L., Yew, W. S., Akana, J., Gerlt, J. A., and Rayment, I. (2005) Biochemistry 44, 1816-1823
33. Yew, W. S., Akana, J., Wise, E. L., Rayment, I., and Gerlt, J. A. (2005) Biochemistry 44, 1807-1815
34. Wise, E. L., Yew, W. S., Gerlt, J. A., and Rayment, I. (2004) Biochemistry 43, 6438-6446
35. Yew, W. S., Wise, E. L., Rayment, I., and Gerlt, J. A. (2004) Biochemistry 43, 6427-6437
36. Weiss, M. S. (2001) J. Appl. Crystallogr. 34, 130-135