Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase

Bioresource Technology

Volumn 101, Issue 23, 2010, Pages 9204-9212

  Merone, Luigia ^a   Mandrich, Luigi ^a   Porzio, Elena ^a   Rossi, Mosé ^a   Müller, Susanne ^b   Reiter, Georg ^b   Worek, Franz ^b   Manco, Giuseppe ^a  

^a INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^b BUNDESWEHR INSTITUTE OF PHARMACOLOGY AND TOXICOLOGY   (Germany)

Author keywords

Directed evolution; Enzyme activation; Nerve agent hydrolysis; Thermostable lactonase

Indexed keywords

ARCHAEAL; CYCLOSARIN; DIRECTED EVOLUTION; ENZYME ACTIVATION; HYDROLASES; LACTONASE; NERVE AGENTS; NERVE GAS; ORGANOPHOSPHORUS COMPOUNDS; PARAOXON; PHOSPHOTRIESTERASES; SINGLE MUTANT; SULFOLOBUS SOLFATARICUS; TERRORIST ACTS; WILD TYPES;

AGRICULTURAL WASTES; DETOXIFICATION; ENZYMES; EVOLUTIONARY ALGORITHMS; HYDROLYSIS; PHOSPHORUS COMPOUNDS;

BIOLOGY;

CYCLOSARIN; GLUCONOLACTONASE; HYDROLASE; LACTONE; ORGANOPHOSPHATE; PARAOXON;

AGRICULTURAL RUNOFF; BIOTERRORISM; DETOXIFICATION; ENZYME ACTIVITY; HYDROLYSIS; NERVOUS SYSTEM; PROKARYOTE;

AGRICULTURAL WASTE; ARTICLE; BREVUNDIMONAS DIMINUTA; DETOXIFICATION; ENZYME ACTIVATION; ENZYME ACTIVITY; HYDROLYSIS; NEUROTOXICITY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; STRESS; SULFOLOBUS SOLFATARICUS; THERMOSTABILITY; WILD TYPE;

BIODEGRADATION, ENVIRONMENTAL; CARBOXYLIC ESTER HYDROLASES; CATALYTIC DOMAIN; CHEMICAL WARFARE AGENTS; DIRECTED MOLECULAR EVOLUTION; ENZYME ASSAYS; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; LIGANDS; MUTATION; PHOSPHORIC TRIESTER HYDROLASES; PROTEIN STRUCTURE, SECONDARY; SODIUM DODECYL SULFATE; SULFOLOBUS ACIDOCALDARIUS; SULFOLOBUS SOLFATARICUS; TEMPERATURE;

ARCHAEA; BREVUNDIMONAS DIMINUTA; SULFOLOBUS SOLFATARICUS;

EID: 77955653933     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.06.102     Document Type: Article

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