Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Gao, Xue ^a   Yuan, Xiao Xi ^a   Shi, Zhen Yu ^b   Guo, Ying Ying ^a   Shen, Xiao Wen ^a   Chen, Jin Chun ^a   Wu, Qiong ^a   Chen, Guo Qiang ^a,c  

^a Tsinghua University   (China)

^b Synthenomecom   (Australia)

^c TSINGHUA UNIVERSITY   (China)

Author keywords

Codon optimization; Escherichia coli; Hairpin; PHA synthase; PHB; Polyhydroxyalkanoates

Indexed keywords

3 HYDROXYALKANOATE; 3 HYDROXYBUTYRIC ACID; HYDROXYACID; POLYESTER; POLYHYDROXYALKANOATE SYNTHASE; UNCLASSIFIED DRUG;

5' UNTRANSLATED REGION; ARTICLE; CODON; ENZYME STRUCTURE; ENZYME SYNTHESIS; ESCHERICHIA COLI; NONHUMAN; WESTERN BLOTTING;

3-HYDROXYBUTYRIC ACID; ACYLTRANSFERASES; BACTERIAL PROTEINS; ESCHERICHIA COLI; HYDROXYBUTYRATES; MUTATION; POLYESTERS; POLYHYDROXYALKANOATES; PSEUDOMONAS STUTZERI; RECOMBINANT PROTEINS; RNA, MESSENGER;

ESCHERICHIA COLI; PSEUDOMONAS STUTZERI;

EID: 84866145428     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-130     Document Type: Article

References (41)

1. Gao X, Chen JC, Wu Q, Chen GQ. Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels. Curr Opin Biotechnol 2011, 22:768-774. 10.1016/j.copbio.2011.06.005, 21705209.
2. Chen GQ, Patel MK. Plastics derived from biological sources: present and future: a technical and environmental review. Chem Rev 2012, 112:2082-2099. 10.1021/cr200162d, 22188473.
3. Tripathi L, Wu LP, Chen J, Chen GQ. Synthesis of Diblock copolymer poly-3-hydroxybutyrate-block-poly-3-hydroxyhexanoate [PHB-b-PHHx] by a beta-oxidation weakened Pseudomonas putida KT2442. Microb Cell Fact 2012, 11:44. 10.1186/1475-2859-11-44, 3442986, 22480145.
4. Zhou XY, Yuan XX, Shi ZY, Meng DC, Jiang WJ, Wu LP, Chen JC, Chen GQ. Hyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli. Microb Cell Fact 2012, 11:54. 10.1186/1475-2859-11-54, 22550959.
5. Zhang S, Wang ZH, Chen GQ. Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification. Microb Cell Fact 2010, 9:28. 10.1186/1475-2859-9-28, 2873406, 20459707.
6. Wang ZH, Ma P, Chen J, Zhang J, Chen CB, Chen GQ. A transferable heterogeneous two-hybrid system in Escherichia coli based on polyhydroxyalkanoates synthesis regulatory protein PhaR. Microb Cell Fact 2011, 10:21. 10.1186/1475-2859-10-21, 3079617, 21477323.
7. Hazer B, Steinbüchel A. Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications. Appl Microbiol Biotechnol 2007, 74:1-12. 10.1007/s00253-006-0732-8, 17146652.
8. Chen GQ. A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Rev 2009, 38:2434-2446. 10.1039/b812677c, 19623359.
9. Steinbüchel A, Lutke-Eversloh T. Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem Eng J 2003, 16:81-96.
10. Doi Y, Kitamura S, Abe H. Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-hydroxyhexanoate). Macromolecules 1995, 28:4822-4828.
11. Eggink G, de Waard P, Huijberts GN. Formation of novel poly(hydroxyalkanoates) from long-chain fatty acids. Can J Microbiol 1995, 41(Suppl 1):14-21.
12. Chen Plastics from Bacteria: Natural Functions and Applications 2010, Germany: Heidelberg, Chen.
13. Matsusaki H, Abe H, Doi Y. Biosynthesis and properties of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant strains of Pseudomonas sp. 61-3. Biomacromolecules 2000, 1:17-22. 10.1021/bm9900040, 11709837.
14. Sudesh K, Abe H, Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog Polym Sci 2000, 25:1503-1555.
15. Jian J, Li ZJ, Ye HM, Yuan MQ, Chen GQ. Metabolic engineering for microbial production of polyhydroxyalkanoates consisting of high 3-hydroxyhexanoate content by recombinant Aeromonas hydrophila. Bioresour Technol 2010, 101:6096-6102. 10.1016/j.biortech.2010.02.089, 20236821.
16. Normi YM, Hiraishi T, Taguchi S, Sudesh K, Najimudin N, Doi Y. Site-directed saturation mutagenesis at residue F420 and recombination with another beneficial mutation of Ralstonia eutropha polyhydroxyalkanoate synthase. Biotechnol Lett 2005, 27:705-712. 10.1007/s10529-005-5186-z, 16049738.
17. Loo CY, Lee WH, Tsuge T, Doi Y, Sudesh K. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from palm oil products in a Wautersia eutropha mutant. Biotechnol Lett 2005, 27:1405-1410. 10.1007/s10529-005-0690-8, 16215858.
18. Tsuge T, Watanabe S, Sato S, Hiraishi T, Abe H, Doi Y, Taguchi S. Variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of Aeromonas caviae PHA synthase. Macromol Biosci 2007, 7:846-854. 10.1002/mabi.200700023, 17541931.
19. Chen JY, Song G, Chen GQ. A lower specificity PhaC2 synthase from Pseudomonas stutzeri catalyses the production of copolyesters consisting of short-chain-length and medium-chain-length 3-hydroxyalkanoates. Antonie Van Leeuwenhoek 2006, 89:157-167. 10.1007/s10482-005-9019-9, 16496091.
20. Shen XW, Shi ZY, Song G, Li ZJ, Chen GQ. Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers. Appl Microbiol Biotechnol 2011, 91:655-665. 10.1007/s00253-011-3274-7, 21509565.
21. Dellomonaco C, Fava F, Gonzalez R. The path to next generation biofuels: successes and challenges in the era of synthetic biology. Microb Cell Fact 2010, 9:3. 10.1186/1475-2859-9-3, 2817670, 20089184.
22. Steinbüchel A, Fuchtenbusch B, Gorenflo V, Hein S, Jossek R, Langenbach S, Rehm BHA. Biosynthesis of polyesters in bacteria and recombinant organisms. Polym Degrad Stabil 1998, 59:177-182.
23. Park SJ, Lee SY. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by metabolically engineered Escherichia coli strains. Appl Biochem Biotechnol 2004, 113:335-346.
24. Matsumoto K, Takase K, Yamamoto Y, Doi Y, Taguchi S. Chimeric enzyme composed of polyhydroxyalkanoate (PHA) synthases from Ralstonia eutropha and Aeromonas caviae enhances production of PHAs in recombinant Escherichia coli. Biomacromolecules 2009, 10:682-685. 10.1021/bm801386j, 19226108.
25. Nomura CT, Taguchi K, Taguchi S, Doi Y. Coexpression of genetically engineered 3-ketoacyl-ACP synthase III (fabH) and polyhydroxyalkanoate synthase (phaC) genes leads to short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production from glucose in Escherichia coli JM109. Appl Environ Microbiol 2004, 70:999-1007. 10.1128/AEM.70.2.999-1007.2004, 348927, 14766582.
26. Nomura CT, Tanaka T, Gan Z, Kuwabara K, Abe H, Takase K, Taguchi K, Doi Y. Effective enhancement of short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production by coexpression of genetically engineered 3-ketoacyl-acyl-carrier-protein synthase III (fabH) and polyhydroxyalkanoate synthesis genes. Biomacromolecules 2004, 5:1457-1464. 10.1021/bm049959v, 15244465.
27. Nomura CT, Tanaka T, Eguen TE, Appah AS, Matsumoto K, Taguchi S, Ortiz CL, Doi Y. FabG mediates polyhydroxyalkanoate production from both related and nonrelated carbon sources in recombinant Escherichia coli LS5218. Biotechnol Prog 2008, 24:342-351. 10.1021/bp070303y, 18215055.
28. Carrier TA, Keasling JD. Controlling messenger RNA stability in bacteria: strategies for engineering gene expression. Biotechnol Prog 1997, 13:699-708. 10.1021/bp970095h, 9413129.
29. Carrier TA, Keasling JD. Library of synthetic 5 ' secondary structures to manipulate mRNA stability in Escherichia coli. Biotechnol Prog 1999, 15:58-64. 10.1021/bp9801143, 9933514.
30. Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: a multi-gene study. Protein Expr Purif 2008, 59:94-102. 10.1016/j.pep.2008.01.008, 18289875.
31. Gao Z, Li Z, Zhang Y, Huang H, Li M, Zhou L, Tang Y, Yao B, Zhang W. High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization. Biotechnol Lett 2012, 34:507-514. 10.1007/s10529-011-0790-6, 22052258.
32. Menzella HG. Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli. Microb Cell Fact 2011, 10:15. 10.1186/1475-2859-10-15, 3056764, 21371320.
33. Li QA, Chen QA, Li MJ, Wang FS, Qi QS. Pathway engineering results the altered polyhydroxyalkanoates composition in recombinant Escherichia coli. New Biotech 2011, 28:92-95.
34. Marchisio MA, Rudolf F. Synthetic biosensing systems. Int J Biochem Cell Biol 2011, 43:310-319. 10.1016/j.biocel.2010.11.012, 21109019.
35. Ellis T, Adie T, Baldwin GS. DNA assembly for synthetic biology: from parts to pathways and beyond. Integr Biol (Camb) 2011, 3:109-118.
36. Puigbo P, Guzman E, Romeu A, Garcia-Vallve S. OPTIMIZER: a web server for optimizing the codon usage of DNA sequences. Nucleic Acids Res 2007, 35:W126-131. 10.1093/nar/gkm219, 1933141, 17439967.
37. Spratt SK, Ginsburgh CL, Nunn WD. Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism. J Bacteriol 1981, 146:1166-1169. 216975, 7016831.
38. Spiekermann P, Rehm BH, Kalscheuer R, Baumeister D, Steinbüchel A. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 1999, 171:73-80. 10.1007/s002030050681, 9914303.
39. Chen JY, Liu T, Zheng Z, Chen JC, Chen GQ. Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities. FEMS Microbiol Lett 2004, 234:231-237. 10.1111/j.1574-6968.2004.tb09538.x, 15135527.
40. Qiu YZ, Han J, Guo JJ, Chen GQ. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from gluconate and glucose by recombinant Aeromonas hydrophila and Pseudomonas putida. Biotechnol Lett 2005, 27:1381-1386. 10.1007/s10529-005-3685-6, 16215853.
41. Kato M, Bao HJ, Kang CK, Fukui T, Doi Y. Production of a novel copolyester of 3-hydroxybutyric acid and medium chain length 3-hydroxyalkanaic acids by Pseudomonas sp 61-3 from sugars. Appl Microbiol Biotechnol 1996, 45:363-370.