Metabolic engineering for microbial production of polyhydroxyalkanoates consisting of high 3-hydroxyhexanoate content by recombinant Aeromonas hydrophila

Bioresource Technology

Volumn 101, Issue 15, 2010, Pages 6096-6102

  Jian, Jia ^a   Li, Zheng Jun ^a   Ye, Hai Mu ^b   Yuan, Mei Qing ^c   Chen, Guo Qiang ^a  

^a TSINGHUA UNIVERSITY   (China)

^b Beihang Unive   (China)

^c INSTITUTE OF FORENSIC SCIENCE   (China)

Author keywords

3 Hydroxyhexanoate; Aeromonas hydrophila; fadD; fadL; Polyhydroxyalkanoates

Indexed keywords

3-HYDROXYBUTYRATE; 3-HYDROXYHEXANOATE; ACYL-COA; AEROMONAS HYDROPHILA; FATTY ACID TRANSPORT; HEXANOATE; IN-CELL; METABOLIC ENGINEERING; MICROBIAL PRODUCTION; NITROGEN LIMITATION; OCTANOATE; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOATES; POLYMER BINDING; PRESSURE SENSITIVE ADHESIVES; PSEUDOMONAS PUTIDA; PSEUDOMONAS STUTZERI; SOLVENT FREE; SYNTHASES; TWO-STEP CULTIVATION;

BIODEGRADABLE POLYMERS; CLONING; ESCHERICHIA COLI; FATTY ACIDS; SODIUM;

ORGANIC POLYMERS;

3 HYDROXYHEXANOIC ACID; FATTY ACID TRANSPORTER; HEXANOIC ACID DERIVATIVE; LONG CHAIN FATTY ACID COENZYME A LIGASE; POLY(3 HYDROXYBUTYRATE CO 3 HYDROXYHEXANOATE CO 3 HYDROXYOCTANOIC ACID); POLY(3 HYDROXYBUTYRATE CO 3 HYDROXYHEXANOIC ACID); POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOIC ACID; UNCLASSIFIED DRUG;

BIODEGRADATION; CLONE; COLIFORM BACTERIUM; GENETIC ENGINEERING; MICROBIAL ACTIVITY;

AEROMONAS HYDROPHILA; ARTICLE; BACTERIAL STRAIN; CHEMICAL COMPOSITION; CONTROLLED STUDY; DRY WEIGHT; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE DELETION; METABOLIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; PSEUDOMONAS STUTZERI; SYNTHESIS;

AEROMONAS HYDROPHILA; HEXANOIC ACIDS; POLYHYDROXYALKANOATES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

AEROMONAS HYDROPHILA; ESCHERICHIA COLI; PSEUDOMONAS PUTIDA; PSEUDOMONAS STUTZERI;

EID: 77951093407     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.02.089     Document Type: Article

References (32)

1. Anderson A.J., Haywood G.W., and Dawes E.A. Biosynthesis and composition of bacterial poly(hydroxyalkanoates). Int. J. Biol. Macromol. 12 (1990) 102-105
2. Babu G.N., Hammer W.J., and Rutherford D.R. Poly-3-hydroxyalkanoates as pressure sensitive adhesives. In: Eggink G., Steinbüchel A., Poirier Y., and Witholt B. (Eds). 1996 International Symposium on Bacterial Polyhydroxyalkanoates (1997), National Research Council of Canada, Ottawa 48-55
3. Cai L., Yuan M.Q., Liu F., Jian J., and Chen G.Q. Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442. Bioresour. Technol. 100 (2009) 2265-2270
4. Chen G.Q. A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem. Soc. Rev. 38 (2009) 2434-2446
5. Chen J.Y., Liu T., Zheng Z., Chen J.C., and Chen G.Q. Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities. FEMS Microbiol. Lett. 234 (2004) 231-237
6. Chung C.W., Kim Y.S., Kim Y.B., Bae K.S., and Rhee Y.H. Isolation of a Pseudomonas sp. strain exhibiting unusual behavior of poly(3-hydroxyalkanoates) biosynthesis and characterization of synthesized polyesters. J. Microbiol. Biotechnol. 9 (1999) 847-853
7. Edwards R.A., Keller L.H., and Schifferli D.M. Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression. Gene 207 (1998) 149-157
8. Fukui T., Shiomi N., and Doi Y. Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae. J. Bacteriol. 180 (1998) 667-673
9. Kameda K., and Nunn W.D. Purification and characterization of acyl coenzyme-A synthetase from Escherichia coli. J. Biol. Chem. 256 (1981) 5702-5707
10. Kellerhals M.B., Kessler B., and Witholt B. Closed-loop control of bacterial high-cell-density fed-batch cultures: production of mcl-PHAs by Pseudomonas putida KT2442 under single-substrate and cofeeding conditions. Biotechnol. Bioeng. 65 (1999) 306-315
11. Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop R.M., and Peterson K.M. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166 (1995) 175-176
12. Lee S.H., Oh D.H., Ahn W.S., Lee Y., Choi J.I., and Lee S.Y. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas hydrophila. Biotechnol. Bioeng. 67 (2000) 240-244
13. Lu J.N., Tappel R.C., and Nomura C.T. Mini-review: biosynthesis of poly(hydroxyalkanoates). Polym. Rev. 49 (2009) 226-248
14. Martin D.P., and Williams S.F. Medical applications of poly-4-hydroxybutyrate: a strong flexible absorbable biomaterial. Biochem. Eng. J. 16 (2003) 97-105
15. Ouyang S.P., Han J., Qiu Y.Z., Qin L.F., Chen S., Wu Q., Leski M.L., and Chen G.Q. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) production in recombinant Aeromonas hydrophila 4AK4 harboring phbA, phbB and vgb genes. Macromol. Symp. 224 (2005) 21-34
16. Ps. Biochem. Eng. J. 37 (2007) 144-150
17. Qiu Y.Z., Ouyang S.P., Shen Z.Y., Wu Q., and Chen G.Q. Metabolic engineering for the production of copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate by Aeromonas hydrophila. Macromol. Biosci. 4 (2004) 255-261
18. Qiu Y.Z., Han J., Guo J.J., and Chen G.Q. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from gluconate and glucose by recombinant Aeromonas hydrophila and Pseudomonas putida. Biotechnol. Lett. 27 (2005) 1381-1386
19. Reddy C.S.K., Ghai R., and Kalia R.V.C. Polyhydroxyalkanoates: an overview. Bioresour. Technol. 87 (2003) 137-146
20. Sauer U., Canonaco F., Heri S., Perrenoud A., and Fischer E. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J. Biol. Chem. 279 (2004) 6613-6619
21. Shen X.W., Yang Y., Jian J., Wu Q., and Chen G.Q. Production and characterization of homopolymer poly(3-hydroxyvalerate) (PHV) accumulated by wild type and recombinant Aeromonas hydrophila strain 4AK4. Bioresour. Technol. 100 (2009) 4296-4299
22. Simon R., Priefer U., and Puhler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Bio/Technology 1 (1983) 784-791
23. Steinbüchel A. Perspectives for biotechnological production and utilization of biopolymers: metabolic engineering of polyhydroxyalkanoate biosynthesis pathways as a successful example. Macromol. Biosci. 1 (2001) 1-24
24. Van der Walle G.A.M., Buisman G.J.H., Weusthuis R.A., and Eggink G. Development of environmentally friendly coatings and paints using medium-chain-length poly(3-hydroxyalkanoates) as the polymer binder. Int. J. Biol. Macromol. 25 (1999) 123-128
25. Wang H.H., Li X.T., and Chen G.Q. Production and characterization of homopolymer polyhydroxyheptanoate (P3HHp) by a fadBA knockout mutant Pseudomonas putida KTOY06 derived from P. putida KT2442. Process Biochem. 44 (2009) 106-111
26. Ward P.G., de Roo G., and O'Connor K.E. Accumulation of polyhydroxyalkanoate from styrene and phenylacetic acid by Pseudomonas putida CA-3. Appl. Environ. Microbiol. 71 (2005) 2046-2052
27. Xie W.P., and Chen G.Q. Production and characterization of terpolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) by recombinant Aeromonas hydrophila 4AK4 harboring genes phaPCJ. Biochem. Eng. J. 38 (2008) 384-389
28. Yu H.B., Zhang Y.L., Lau Y.L., Yao F., Vilches S., Merino S., Tomas J.M., Howard S.P., and Leung K.Y. Identification and characterization of putative virulence genes and gene clusters in Aeromonas hydrophila PPD134/91. Appl. Environ. Microbiol. 71 (2005) 4469-4477
29. Yuan M.Q., Shi Z.Y., Wei X.X., Wu Q., Chen S.F., and Chen G.Q. Microbial production of medium-chain-length 3-hydroxyalkanoic acids by recombinant Pseudomonas putida KT2442 harboring genes fadL, fadD and phaZ. FEMS Microbiol. Lett. 283 (2008) 167-175
30. Zhang L., Shi Z.Y., Wu Q., and Chen G.Q. Microbial production of 4-hydroxybutyrate, poly-4-hydroxybutyrate, and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by recombinant microorganisms. Appl. Microbiol. Biotechnol. 84 (2009) 909-916
31. Zhao W., and Chen G.Q. Production and characterization of terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by recombinant Aeromonas hydrophila 4AK4 harboring genes phaAB. Process Biochem. 42 (2007) 1342-1347
32. Zheng Z., Li M., Xue X.J., Tian H.L., Li Z., and Chen G.Q. Mutation on N-terminus of polyhydroxybutyrate synthase of Ralstonia eutropha enhanced PHB accumulation. Appl. Microbiol. Biotechnol. 72 (2006) 896-905