Chimeric enzyme composed of polyhydroxyalkanoate (PHA) synthases from ralstonia eutropha and aeromonas caviae enhances production of PHAs in recombinant escherichia coli

Biomacromolecules

Volumn 10, Issue 4, 2009, Pages 682-685

  Matsumoto, Ken'Ichiro ^a   Takase, Kazuma ^b   Yamamoto, Yoko ^a   Doi, Yoshiharu ^b   Taguchi, Seiichi ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSTS; ESCHERICHIA COLI;

3-HYDROXYBUTYRATE; 3-HYDROXYHEXANOATE; AEROMONAS; CHAIN-LENGTH; CHIMERIC ENZYMES; E. COLI; ENZYMATIC ACTIVITIES; ENZYMATIC PROPERTIES; ESCHERICHIA COLI JM109; N TERMINALS; PHA PRODUCTIONS; PHA SYNTHASE; PHA SYNTHASES; POLY-HYDROXYALKANOATE; RALSTONIA EUTROPHA; RECOMBINANT ESCHERICHIA COLI; SHORT CHAIN LENGTHS; SUBSTRATE SPECIFICITIES; SYNTHASES;

ENZYMES;

3 HYDROXYBUTYRIC ACID; BACTERIAL ENZYME; CHIMERIC PROTEIN; HEXANOIC ACID DERIVATIVE; POLYHYDROXYALKANOATE SYNTHASE; ACYLTRANSFERASE; HYBRID PROTEIN; POLY(3-HYDROXYALKANOIC ACID) SYNTHASE; POLYHYDROXYALKANOIC ACID; PRIMER DNA; RECOMBINANT DNA;

AEROMONAS PUNCTATA; AMINO TERMINAL SEQUENCE; CARBOXY TERMINAL SEQUENCE; CONFERENCE PAPER; CUPRIAVIDUS NECATOR; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; AEROMONAS; AMINO ACID SEQUENCE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; IMMUNOBLOTTING; METABOLISM; MOLECULAR GENETICS; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY;

AEROMONAS PUNCTATA; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

ACYLTRANSFERASES; AEROMONAS; AMINO ACID SEQUENCE; CUPRIAVIDUS NECATOR; DNA PRIMERS; DNA, RECOMBINANT; ESCHERICHIA COLI; GENETIC ENGINEERING; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; POLYHYDROXYALKANOATES; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 65249162210     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm801386j     Document Type: Conference Paper

References (32)

1. Doi, Y. Microbial Polyesters; VHC Publishers: New York, 1990.
2. Stubbe, J.; Tian, J.; He, A.; Sinskey, A. J.; Lawrence, A. G.; Liu, P. Annu. Rev. Biochem. 2005, 74, 433-180.
3. Garlotta, D. J. Polym. Environ. 2001, 9, 63-83.
4. Doi, Y.; Segawa, A.; Kunioka, M. Int. J. Biol. Macromol. 1990, 12, 106-111.
5. Lütke-Eversloh, T.; Fischer, A.; Remminghorst, U.; Kawada, J.; Marchessault, R. H.; Bogershausen, A.; Kalwei, M.; Eckert, H.; Reichelt, R.; Liu, S. J.; Steinbuchel, A. Nat. Mater. 2002, 1, 236-240.
6. Renard, E.; Poux, A.; Timbart, L.; Langlois, V.; Guerin, P. Biomac- romolecules 2005, 6, 891-896.
7. Matsusaki, H.; Abe, H.; Doi, Y. Biomacromolecules 2000, 1, 17-22.
8. Taguchi, S.; Tsuge, T. In Protein Engineering Handbook; Lutg, S., Boruscheuer U. T., Eds.; Wiley-VCH: Weinheim, 2008; p 877.
9. Kichise, T.; Taguchi, S.; Doi, Y. Appl. Environ. Microbiol. 2002, 68, 2411-2419.
10. Normi, Y. M.; Hiraishi, T.; Taguchi, S.; Abe, H.; Sudesh, K.; Najimudin, N.; Doi, Y. Macromol. Biosci. 2005, 5, 197-206.
11. Takase, K.; Taguchi, S.; Doi, Y. J. Biochem. 2003, 133, 139-145.
12. Tsuge, T.; Watanabe, S.; Sato, S.; Hiraishi, T.; Abe, H.; Doi, Y.; Taguchi, S. Macromol. Biosci. 2007, 7, 846-854.
13. Jo, S. J.; Matsumoto, K.; Leong, C. R.; Ooi, T.; Taguchi, S. J. Biosci. Bioeng. 2007, 104, 457-463.
14. Matsumoto, K.; Nagao, R.; Murata, T.; Arai, Y.; Kichise, T.; Nakashita, H.; Taguchi, S.; Shimada, H.; Doi, Y. Biomacromolecules 2005, 6, 2126-2130.
15. Takase, K.; Matsumoto, K.; Taguchi, S.; Doi, Y. Biomacromolecules 2004, 5, 480-485.
16. Taguchi, S.; Yamada, M.; Matsumoto, K.; Tajima, K.; Satoh, Y.; Munekata, M.; Ohno, K.; Kohda, K.; Shimamura, T.; Kambe, H.; Obata, S. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 17323-17327.
17. Tsuge, T.; Hisano, T.; Taguchi, S.; Doi, Y. Appl. Environ. Microbiol. 2003, 69, 4830-4836.
18. Nomura, C. T.; Taguchi, S. Appl. Microbiol. Biotechnol. 2007, 73, 969-979.
19. Taguchi, S.; Doi, Y. Macromol. Biosci. 2004, 4, 146-156.
20. Sheu, D. S.; Lee, C. Y. J. Bacteriol. 2004, 186, 4177-4184.
21. Rehm, B. H. A.; Antonio, R. V.; Spiekermann, P.; Amara, A. A.; Steinbüchel, A. Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol. 2002, 1594, 178-190.
22. Solaiman, D. K. Y.; Ashby, R. D.; Foglia, T. A. Biotechnol. Lett. 2002, 24, 1011-1016.
23. Niamsiri, N.; Delamarre, S. C.; Kim, Y. R.; Batt, C. A. Appl. Environ. Microbiol. 2004, 70, 6789-6799.
24. Schubert, P.; Steinbuchel, A.; Schlegel, H. G. J. Bacteriol. 1988,170, 5837-5847.
25. Slater, S.; Voige, W. H.; Dennis, D. E. J. Bacteriol. 1998, 170, 4431-4436.
26. Fukui, T.; Doi, Y. J. Bacteriol. 1997, 179, 4821-4830.
27. Kato, M.; Bao, H. J.; Kang, C. K.; Fukui, T.; Doi, Y. Appl. Microbiol. Biotechnol. 1996, 45, 363-370.
28. Kusaka, S.; Abe, H.; Lee, S. Y.; Doi, Y. Appl. Microbiol. Biotechnol. 1997, 47, 140-143.
29. Fukui, T.; Yokomizo, S.; Kobayashi, G.; Doi, Y. FEMS Microbiol. Lett. 1999, 170, 69-75.
30. Matsumoto, K.; Takase, K.; Aoki, E.; Doi, Y.; Taguchi, S. Biomac-romolecules 2005, 6, 99-104.
31. Zheng, Z.; Li, M.; Xue, X. J.; Tian, H. L.; Li, Z.; Chen, G. Q. Appl. Microbiol. Biotechnol. 2006, 72, 896-905.
32. Ye, Z. Q.; Song, G.; Chen, G. Q.; Chen, J. Y. Biochem. Eng. J. 2008, 41, 67-73.