A transferable heterogeneous two-hybrid system in Escherichia coli based on polyhydroxyalkanoates synthesis regulatory protein PhaR

Microbial Cell Factories

Volumn 10, Issue , 2011, Pages

  Wang, Zhi Hui ^a   Ma, Ping ^a   Chen, Jiong ^a   Zhang, Jing ^a   Chen, Chong Bo ^a   Chen, Guo Qiang ^b  

^a SHANTOU UNIVERSITY   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR 2; BACTERIAL PROTEIN; BETA GALACTOSIDASE; POLYHYDROXYALKANOATE SYNTHASE; PROTEIN C JUN; PROTEIN FOS; REGULATOR PROTEIN; ESCHERICHIA COLI PROTEIN; POLYHYDROXYALKANOIC ACID;

ARTICLE; CONTROL GROUP; DNA BINDING; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FEASIBILITY STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN INTERACTION; REPORTER GENE; TWO HYBRID SYSTEM; CHEMISTRY; EVALUATION; GENETICS; METABOLISM; PROMOTER REGION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; POLYHYDROXYALKANOATES; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 79953732536     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-10-21     Document Type: Article

References (23)

1. Suter B, Kittanakom S, Stagljar I. Two-hybrid technologies in proteomics research. Curr Opin Biotechnol 2008, 19:316-323. 10.1016/j.copbio.2008.06.005, 18619540.
2. Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc Natl Acad Sci USA 1998, 95:5752-5756. 10.1073/pnas.95.10.5752, 20451, 9576956.
3. Chien C, Bartel P, Sternglanz R, Fields S. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci USA 1991, 88:9578-9582. 10.1073/pnas.88.21.9578, 52761, 1946372.
4. Allen J, Walberg M, Edwards M, Elledge S. Finding prospective partners in the library: the two-hybrid system and phage display find a match. Trends Biochem Sci 1995, 20:511-516. 10.1016/S0968-0004(00)89119-7, 8571453.
5. Strauch EM, Georgiou G. A bacterial two-hybrid system based on the twin-arginine transporter pathway of E. coli. Protein Sci 2007, 16:1001-1008. 10.1110/ps.062687207, 2206650, 17456749.
6. Clarke P, Cuív P, O'Connell M. Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation. Nucleic Acids Res 2005, 33:e18. 10.1093/nar/gni011, 548371, 15687376.
7. Anderson A, Dawes E. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 1990, 54:450-472. 372789, 2087222.
8. Madison L, Huisman G. Metabolic engineering of poly (3-hydroxyalkanoates): from DNA to plastic. Microbiol Mol Biol Rev 1999, 63:21-53. 98956, 10066830.
9. Leaf T, Peterson M, Stoup S, Somers D, Srienc F. Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate. Microbiology 1996, 142:1169-1180. 10.1099/13500872-142-5-1169, 8704958.
10. Maehara A, Yamane T, Taguchi S, Doi Y. Molecular characterization of a regulatory protein (PhaR) involved in PHA biosynthesis. RIKEN Rev 2001, 42:77-80.
11. Maehara A, Taguchi S, Nishiyama T, Yamane T, Doi Y. A repressor protein, PhaR, regulates polyhydroxyalkanoate (PHA) synthesis via its direct interaction with PHA. J Bacteriol 2002, 184:3992-4002. 10.1128/JB.184.14.3992-4002.2002, 135160, 12081972.
12. Yamada M, Yamashita K, Wakuda A, Ichimura K, Maehara A, Maeda M, Taguchi S. Autoregulator protein PhaR for biosynthesis of polyhydroxybutyrate [P (3HB)] possibly has two separate domains that bind to the target DNA and P (3HB): functional mapping of amino acid residues responsible for DNA binding. J Bacteriol 2007, 189:1118-1127. 10.1128/JB.01550-06, 1797304, 17122335.
13. Kerppola TK. Visualization of molecular interactions by fluorescence complementation. Nat Rev Mol Cell Biol 2006, 7:449-456. 10.1038/nrm1929, 2512262, 16625152.
14. Kerppola T, Curran T. Selective DNA bending by a variety of bZIP proteins. Mol Cell Biol 1993, 13:5479-5489. 360261, 8355695.
15. Hu CD, Kerppola TK. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol 2003, 21:539-545. 10.1038/nbt816, 1820765, 12692560.
16. Spiekermann P, Rehm BHA, Kalscheuer R, Baumeister D, Steinbüchel A. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 1999, 171:73-80. 10.1007/s002030050681, 9914303.
17. Wang ZH, Wu HN, Chen J, Zhang J, Yao YC, Chen GQ. A novel self-cleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles. Lab Chip 2008, 8:1957-1962. 10.1039/b807762b, 18941699.
18. O'Shea E, Rutkowski R, Stafford W, Kim P. Preferential heterodimer formation by isolated leucine zippers from fos and jun. Science 1989, 245:646-648.
19. Villarreal X, Richter J. Analysis of ATF2 gene expression during early Xenopus laevis development. Gene 1995, 153:225-229. 10.1016/0378-1119(94)00770-S, 7875593.
20. Wieczorek R, Pries A, Steinbüchel A, Mayer F. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J Bacteriol 1995, 177:2425-2435. 176901, 7730274.
21. Kusaka S, Abe H, Lee S, Doi Y. Molecular mass of poly [(R)-3-hydroxybutyric acid] produced in a recombinant Escherichia coli. Appl Microbiol Biotechnol 1997, 47:140-143. 10.1007/s002530050902, 9077002.
22. Duncan PW, Propst M, Nelson SG. Reliability of the Fugl-Meyer assessment of sensorimotor recovery following cerebrovascular accident. Phys Ther 1983, 63:1606-1610.
23. Joung JK, Ramm EI, Pabo CO. A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci USA 2000, 97:7382-7387. 10.1073/pnas.110149297, 16554, 10852947.