Hyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Zhou, Xiao Yun ^a   Yuan, Xiao Xi ^a   Shi, Zhen Yu ^b   Meng, De Chuang ^a   Jiang, Wen Jun ^a   Wu, Lin Ping ^c   Chen, Jin Chun ^a   Chen, Guo Qiang ^a,d  

^a Tsinghua University   (China)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

^d TSINGHUA UNIVERSITY   (China)

Author keywords

4 hydroxybutyrate; Escherichia coli; Metabolic engineering; PhaP; PHB; Poly(4HB); Polyhydroxyalkanoates; Synthetic biology

Indexed keywords

GLUCOSE; HYDROXYBUTYRIC ACID; OXIDOREDUCTASE; POLY(4 HYDROXYBUTYRATE); PROTEIN PHAP1; PROTEIN PHAP2; PROTEIN PHAP3; PROTEIN PHAP4; SUCCINIC ACID; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; CARBON NUCLEAR MAGNETIC RESONANCE; CARBON SOURCE; CHEMICAL STRUCTURE; CLOSTRIDIUM KLUYVERI; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; FERMENTATION; GABD GENE; GAS CHROMATOGRAPHY; NONHUMAN; PROTON NUCLEAR MAGNETIC RESONANCE; SAD GENE;

ACYLTRANSFERASES; CLOSTRIDIUM KLUYVERI; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERMENTATION; GLUCOSE; METABOLIC NETWORKS AND PATHWAYS; PLASMIDS; POLYESTERS; RECOMBINANT PROTEINS; SUCCINATE-SEMIALDEHYDE DEHYDROGENASE; SUCCINIC ACID;

CLOSTRIDIUM KLUYVERI; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

EID: 84871407044     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-54     Document Type: Article

References (48)

1. Anderson AJ, Dawes EA. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 1990, 54:450-472. 372789, 2087222.
2. Steinbüchel A, Valentin HE. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol Lett 1995, 128:219-228.
3. Chen GQ, Patel MK. Plastics derived from biological sources: present and future: a technical and environmental review. Chemical Rev 2012, 112:2082-2099.
4. Cai L, Tan D, Aibaidula G, Dong XR, Chen JC, Tian WD, Chen GQ. Comparative genomics study of PHA and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships. Microb Cell Fact 2011, 10:88. 10.1186/1475-2859-10-88, 3227634, 22040376.
5. Martin DP, Williams SF. Medical applications of poly-4-hydroxybutyrate: a strong flexible absorbable biomaterial. Biochem Eng J 2003, 16:97-105.
6. Hazer B, Steinbüchel A. Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications. Appl Microbiol Biotechnol 2007, 74:1-12. 10.1007/s00253-006-0732-8, 17146652.
7. Chen GQ. A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Rev 2009, 38:2434-2446. 10.1039/b812677c, 19623359.
8. Madison LL, Huisman GW. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol Mol Biol Rev 1999, 63:21-53. 98956, 10066830.
9. Gao X, Chen JC, Wu Q, Chen GQ. Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels. Curr Opin Biotechnol 2011, 22:768-774. 10.1016/j.copbio.2011.06.005, 21705209.
10. Chen GQ, Wu Q. Microbial production and applications of chiral hydroxyalkanoates. Appl Microbiol Biotechnol 2005, 67:592-599. 10.1007/s00253-005-1917-2, 15700123.
11. Tripathi L, Wu LP, Chen GQ. Microbial Synthesis of Diblock Copolymer Poly-3-Hydroxybutyrate-block-Poly-3-Hydroxyhexanoate [P(3HB)-b-P(3HHx)] by a Genome Reduced Pseudomonas putida KT2442. Microb Cell Fact 2012, 11:44. 10.1186/1475-2859-11-44, 3442986, 22480145.
12. Cheng S, Chen GQ, Leski M, Zou B, Wang Y, Wu Q. The effect of D, L-β-hydroxybutyric acid on cell death and proliferation in L929 cells. Biomaterials 2006, 27:3758-3765. 10.1016/j.biomaterials.2006.02.046, 16549197.
13. Sun J, Dai ZW, Chen GQ. Oligomers of polyhydroxyalkanoates stimulated calcium ion channels in mammalian cells. Biomaterials 2007, 28:3896-3903. 10.1016/j.biomaterials.2007.05.011, 17574664.
14. Saito Y, Nakamura S, Hiramitsu M, Doi Y. Microbial Synthesis and Properties of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Polym Int 1996, 39:169-174.
15. Mergaert J, Webb A, Anderson C, Wouters A, Swings J. Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soils. Appl Environ Microbiol 1993, 59:3233-3238. 182442, 8250550.
16. Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials 2005, 26:6565-6578. 10.1016/j.biomaterials.2005.04.036, 15946738.
17. Kunioka M, Nakamura Y, Doi Y. New bacterial copolyesters produced in Alcaligenes eutrophus from organic-acids. Polymer Comm 1988, 29:174-176.
18. Steinbüchel A, Valentin HE, Schönebaum A. Application of recombinant gene technology for production of polyhydroxyalkanoic acids: biosynthesis of poly(4-hydroxybutyric acid) homopolyester. J Polymer Environ 1994, 2:67-74.
19. Nakamura S, Doi Y, Scandola M. Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Macromolecules 1992, 25:4237-4241.
20. Sudesh K, Fukui T, Taguchi K, Iwata T, Doi Y. Improved production of poly(4-hydroxybutyrate) by Comamonas acidovorans and its freeze-fracture morphology. Int J Biol Macromol 1999, 25:79-85. 10.1016/S0141-8130(99)00018-5, 10416653.
21. Choi MH, Yoon SC, Lenz RW. Production of poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid) and poly(4-hydroxybutyric acid) without subsequent degradation by Hydrogenophaga pseudoflava. Appl Environ Microbiol 1999, 65:1570-1577. 91222, 10103252.
22. Hein S, Sohling B, Gottschalk G, Steinbüchel A. Biosynthesis of poly(4-hydroxybutyric acid) by recombinant strains of Escherichia coli. FEMS Microbiol Lett 1997, 153:411-418. 10.1016/S0378-1097(97)00282-6, 9271870.
23. Song SS, Hein S, Steinbüchel A. Production of poly(4-hydroxybutyric acid) by fed-batch cultures of recombinant strains of Escherichia coli. Biotechnol Lett 1999, 21:193-197.
24. Lee WH, Azizan MNM, Sudesh K. Effects of culture conditions on the composition of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) synthesized by Comamonas acidovorans. Polymer Degrad Stabil 2004, 84:129-134.
25. Song SS, Ma H, Gao ZX, Jia ZH, Zhang X. Construction of recombinant Escherichia coli strains producing poly (4-hydroxybutyric acid) homopolyester from glucose. Wei Sheng Wu Xue Tong Bao 2005, 45:382-386.
26. Singh M, Patel SKS, Kalia VC. Bacillus subtilis as potential producer for polyhydroxyalkanoates. Microb Cell Fact 2009, 8:38. 10.1186/1475-2859-8-38, 2719590, 19619289.
27. Valentin HE, Dennis D. Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose. J Biotechnol 1997, 58:33-38. 10.1016/S0168-1656(97)00127-2, 9335177.
28. Li ZJ, Shi ZY, Jian J, Guo YY, Wu Q, Chen GQ. Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered Escherichia coli. Metab Eng 2010, 12:352-359. 10.1016/j.ymben.2010.03.003, 20304089.
29. Sohling B, Gottschalk G. Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri. J Bacteriol 1996, 178:871-880. 177737, 8550525.
30. Spyros A, Marchessault RH. Nuclear magnetic-relaxation measurements of poly(4-hydroxybutyrate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in the bulk. Macromolecules 1995, 28:6108-6111.
31. Valentin HE, Zwingmann G, Schonebaum A, Steinbüchel A. Metabolic pathway for biosynthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from 4-hydroxybutyrate byAlcaligenes eutrophus. Eur J Biochem 1995, 227:43-60. 10.1111/j.1432-1033.1995.tb20358.x, 7851418.
32. Zhang S, Wang ZH, Chen GQ. Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification. Microb Cell Fact 2010, 9:28. 10.1186/1475-2859-9-28, 2873406, 20459707.
33. Wang ZH, Ma P, Chen J, Zhang J, Yao YC, Zhang HF, Chen GQ. A heterogeneous two-hybrid system inEscherichia colibased on polyhydroxyalkanoates synthesis regulatory proteins PhaR. Microb Cell Fact 2011, 10:21. 10.1186/1475-2859-10-21, 3079617, 21477323.
34. Dennis D, Liebig C, Holley T, Thomas KS, Khosla A, Wilson D, Augustine B. Preliminary analysis of polyhydroxyalkanoate inclusions using atomic force microscopy. FEMS Microbiol Lett 2003, 226:113-119. 10.1016/S0378-1097(03)00610-4, 13129616.
35. Wieczorek R, Pries A, Steinbüchel A, Mayer F. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J Bacteriol 1995, 177:2425-2435. 176901, 7730274.
36. Potter M, Muller H, Steinbüchel A. Influence of homologous phasins (PhaP) on PHA accumulation and regulation of their expression by the transcriptional repressor PhaR in Ralstonia eutropha H16. Microbiology 2005, 151:825-833. 10.1099/mic.0.27613-0, 15758228.
37. Lux G, Stabenow-Lohbauer U, Langer M, Bozkurt T. Gastroenterology in Germany determination of current status and perspectives. Results of a survey among members of the German Society of Digestive and Metabolic Diseases. Z Gastroenterol 1996, 34:542-548.
38. Potter M, Muller H, Reinecke F, Wieczorek R, Fricke F, Bowien B, Friedrich B, Steinbüchel A. The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha. Microbiology 2004, 150:2301-2311. 10.1099/mic.0.26970-0, 15256572.
39. Potter M, Steinbüchel A. Poly(3-hydroxybutyrate) granule-associated proteins: impacts on poly(3-hydroxybutyrate) synthesis and degradation. Biomacromolecules 2005, 6:552-560. 10.1021/bm049401n, 15762612.
40. Neumann L, Spinozzi F, Sinibaldi R, Rustichelli F, Potter M, Steinbüchel A. Binding of the major phasin, PhaP1, from Ralstonia eutropha H16 to poly(3-hydroxybutyrate) granules. J Bacteriol 2008, 190:2911-2919. 10.1128/JB.01486-07, 2293264, 18223073.
41. Kunioka M, Kawaguchi Y, Doi Y. Production of biodegradable copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate by Alcaligenes eutrophus. Appl Microbiol Biotechnol 1989, 30:569-573.
42. Mitomo H, Hsieh WC, Nishiwaki K, Kasuya K, Doi Y. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) produced by Comamonas acidovorans. Polymer 2001, 42:3455-3461.
43. Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Potter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbüchel A, Friedrich B, Bowien B. Genome sequence of the bioplastic-producing " Knallgas" bacterium Ralstonia eutropha H16. Nat Biotechnol 2006, 24:1257-1262. 10.1038/nbt1244, 16964242.
44. Kato M, Bao HJ, Kang CK, Fukui T, Doi Y. Production of a novel copolyester of 3-hydroxybutyric acid and medium chain length 3-hydroxyalkanaic acids by Pseudomonas sp 61-3 from sugars. Appl Microbiol Biotechnol 1996, 45:363-370.
45. Chen JY, Liu T, Zheng Z, Chen JC, Chen GQ. Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities. FEMS Microbiol Lett 2004, 234:231-237. 10.1111/j.1574-6968.2004.tb09538.x, 15135527.
46. Ouyang SP, Luo RC, Chen SS, Liu Q, Chung A, Wu Q, Chen GQ. Production of polyhydroxyalkanoates with high 3-hydroxydodecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442. Biomacromolecules 2007, 8:2504-2511. 10.1021/bm0702307, 17661516.
47. Gao Y, Kong L, Zhang L, Gong YD, Chen GQ, Zhao NM, Zhang XF. Improvement of mechanical properties of poly(DL-lactide) films by blending of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Eur Polymer J 2006, 42:764-775.
48. Luo R, Chen J, Zhang L, Chen G. Polyhydroxyalkanoate copolyesters produced by Ralstonia eutropha PHB - 4 harboring a low-substrate-specificity PHA synthase PhaC2Ps from Pseudomonas stutzeri 1317. Biochem Eng J 2006, 32:218-225.