A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds

Archives of Microbiology

Volumn 171, Issue 2, 1999, Pages 73-80

  Spiekermann, Patricia ^a   Rehm, Bernd H A ^a   Kalscheuer, Rainer ^a   Baumeister, Dirk ^a   Steinbüchel, Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Acinetobacter calcoaceticus; Azotobacter vinelandii; Lipid inclusions; Nile blue A; Nile red; Poly(3 hydroxyoctanoic acid); Poly(3 hydroxybutyric acid); Poly(4 hydroxybutyric acid); Polyhydroxyalkanoic acid; Pseudomonas putida; Ralstonia eutropha; Rhodococcus opacus; Triacylglycerols; Viable colony staining

Indexed keywords

OXAZINE DERIVATIVE; POLY(3 HYDROXYBUTYRIC ACID); POLYHYDROXYALKANOIC ACID;

ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL GROWTH; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; CULTURE MEDIUM; ESCHERICHIA COLI; LIPID STORAGE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; SCREENING; STAINING; WAUTERSIA EUTROPHA;

AGAR; BACTERIOLOGICAL TECHNIQUES; COLONY COUNT, MICROBIAL; CULTURE MEDIA; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; HYDROXYBUTYRATES; OXAZINES; POLYESTERS; STAINING AND LABELING;

EID: 0033037037     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002030050681     Document Type: Article

References (37)

1. Alvarez HM, Mayer F, Fabritius D, Steinbüchel A (.1996) Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630. Arch Microbiol 165:377-386
2. Alvarez HM, Kaischeuer R, Steinbüchel A (1997) Accumulation of storage lipids in species of Rhodococcus and Nocardia and effect of inhibitors and polyethylene glycol. Fett/Lipid 99: 239-246
3. Anderson AJ, Dawes EA (1990) Occurrence, metabolism, metabolic role, and industrial uses of bacterial poyhydroxyalkanoates. Microb Rev 54:450-472
4. Bullock WO, Fernandez JM, Short JM (1987) XL1-Blue: high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 5:376-378
5. Cain AJ (1947) The use of Nile Blue in the examination of lipids. Q J Microsc Sci 88:383-392
6. Degelau A, Scheper T, Bailey JE, Guske C (1995) Fluorometric measurement of poly-β-hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry. Appl Microbiol Biotechnol 42:653-657
7. DiRusso CC (1990) Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homoloay to eucaryotic enzymes. J Bacteriol 172:6459-6468
8. Ertesvåg H, Doseth B, Larsen B, Skjåk-Bræk G, Valla S (1994) Cloning and sequencing of an Azotobacter vinelandii mannuronan C-5-epimerase gene. J Bacteriol 176:2846-2853
9. Fowler SD, Greenspan P (1985) Application of Nile Red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections. J Histochem 33:833-836
10. Greenspan P, Mayer EP, Fowler SD (1985) Nile Red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol 100:965-973
11. Haywood GW, Anderson AJ, Williams DR, Dawes EA, Ewing DF (1991) Accumulation of a poly(hydroxyalkanoate) copolymer containing 3-hydroxyvalerate from simple carbohydrate substrates by Rhodococcus sp. NCIMB 40126. Int J Biol Macromol 13:83-88
12. Hein S, Söhling B, Gottschalk G, Steinbüchel A (1997) Biosynthesis of poly(4-hydroxybutyric acid) by recombinant strains of Escherichia coli. FEMS Microbiol Lett 153:411-418
13. Hocking PJ, Marchessault RH (1994) Biopolyesters. In: Griffin GJL (Ed) Chemistry and technology of biodegradable polymers, 1st edn. Chapman & Hall, London, pp 48-96
14. Huisman GW, Wonink E, Meima R, Kazemier B, Terpstra P, Witholt B (1991) Metabolism of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans: identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA. J Biol Chem 266:2191-2198
15. Jendrossek D, Schirmer A, Schlegel HG (1996) Biodegradation of polyhydroxyalkanoic acids. Appl Microbiol Biotechnol 46: 451-463
16. Juni E, Janik A (1969) Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). J Bacteriol 98:281-288
17. Kranz RG, Gabbert KK, Madigan MT (1997) Positive selection systems for discovery of novel polyester biosynthesis genes based on fatty acid detoxification. Appl Environ Microbiol 63:3010-3013
18. Ostle AG, Holt JG (1982) Nile Blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 44:238-241
19. Pieper-Fürst U, Madkour MH, Mayer F, Steinbüchel A (1994) Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber. J Bacteriol 176:4328-4337
20. Poirier Y, Dennis E, Klomparens K, Somerville C (1992) Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. Science 256:320-523
21. Rehm BHA, Krüger N, Steinbüchel A (1998) A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. The phaG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier proteincoenzyme A transferase. J Biol Chem 273:24044-24051
22. Reiser SE (1996) Genetic analysis of wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus strain BD413. PhD Thesis, Michigan Stale University, East Lansing, MI
23. Reiser S, Somerville C (1997) Isolation of mutants of Acinetobacter calcoaceticus deficient in wax ester synthesis and complementation of one mutant with a gene encoding a fatty acyl coenzyme A reductase. J Bacteriol 179:2969-2975
24. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Schlegel HG, Kaltwasser H, Gottschalk G (1961) Ein Submersverfahren zur Kultur Wasserstoff oxydierender Bakterien: wachstumsphysiologische Untersuchungen. Arch Mikrobiol 38:209-222
26. Schlegel HG, Lafferty R, Krauss I (1970) The isolation of mutants not accumulating poly-β-hydroxybutyric acid. Arch Mikrobiol 71:283-294
27. Schubert P, Steinbüchel A, Schlegel HG (1988) Cloning of the Alcaligenes eutrophus genes for synthesis of poly-β-hydroxybutyric acid and synthesis of PHB in Escherichia coll. J Bacteriol 170:5837-5847
28. Segura D, Espin G (1998) Mutational inactivation of a gene homologous to Escherichia colt ptsP affects poly-β-hydroxybutyrate accumulation and nitrogen fixation in Azotobacter vinelandii. J Bacteriol 180:4790-4798
29. Steinbüchel A (1991) Polyhydroxyalkanoic acids. In: Byrom D (ed) Biomaterials. Macmillan, London, pp 123-213
30. Steinbüchel A (1996) PHB and other polyhydroxyalkanoic acids. In: Rehm HJ, Reed G, Pühler A, Stadler P (eds) Biotechnology, vol 6, pp 403-464
31. Steinbüchel A, Füchtenbusch B (1998) Bacterial and other biological systems for polyester production. Trends Biotechnol 16: 419-427
32. Steinbüchel A, Schlegel HG (1991) Genetics of poly(β-hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus. Mol Microbiol 5:535-542
33. Steinbüchel A, Valentin HE (1995) Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol Lett 128:219-228
34. Sun W, Cao JG, Teng K, Meighen EA (1994) Biosynthesis of poly-3-hydroxybutyrate in the luminescent bacterium, Vibrio harveyi, and regulation by the lux autoinducer, N-(3-hydroxybutanoyl)homoserine lactone. J Biol Chem 269:20785-20790
35. Thompson SW (1966) Selected histochemical and histopathological methods. Thomas. Springfield, IL
36. Wang BH, Lu ZX, Polya GM (1997) Inhibition of eukaryote protein kinases by isoquinoline and oxazine alkaloids. Planta Medica 63:494-198
37. Worsey MJ, Williams PA (1975) Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. J Bacteriol 124:7-13