Single-protein nanomechanical mass spectrometry in real time

Nature Nanotechnology

Volumn 7, Issue 9, 2012, Pages 602-608

  Hanay, M S ^a   Kelber, S ^a   Naik, A K ^a,c   Chi, D ^a   Hentz, S ^a,b   Bullard, E C ^a   Colinet, E ^a,b   Duraffourg, L ^b   Roukes, M L ^a  

^a California Institute of Technology   (United States)

^b CEA GRENOBLE   (France)

^c INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

MOLECULES; RESONATORS;

ANTIBODY COMPLEXES; MASS SPECTRA; MULTIMODES; NANO ELECTROMECHANICAL SYSTEMS; NANOMECHANICAL; NEUTRAL SPECIES; SINGLE MOLECULE; VIBRATIONAL MODES;

MASS SPECTROMETRY;

GOLD NANOPARTICLE; IMMUNOGLOBULIN M;

ARTICLE; ELECTRON MICROSCOPY; MASS SPECTROMETRY; MOLECULAR MECHANICS; PRIORITY JOURNAL; SINGLE MOLECULE NANOELECTROMECHANICAL SYSTEM BASED MASS SPECTROMETRY;

EID: 84866079810     PISSN: 17483387     EISSN: 17483395     Source Type: Journal    
DOI: 10.1038/nnano.2012.119     Document Type: Article

References (41)

1. Andersson, C-O. Mass spectrometric studies on amino acid and peptide derivatives. Acta Chem. Scand. 12, 1353 (1958).
2. Beynon, J. H. The use of the mass spectrometer for the identification of organic compounds. Microchim. Acta 44, 437-453 (1956).
3. Domon, B. & Aebersold, R. Mass spectrometry and protein analysis. Science 312, 212-217 (2006).
4. Benesch, J. L. P. & Robinson, C. V. Mass spectrometry of macromolecular assemblies: preservation and dissociation. Curr. Opin. Struct. Biol. 16, 245-251 (2006).
5. Robinson, C. V., Benesch, J. L. P., Ruotolo, B. T. & Simmons, D. A. Protein complexes in the gas phase: technology for structural genomics and proteomics. Chem. Rev. 107, 3544-3567 (2007). (Pubitemid 47322744)
6. Warscheid, B., Oeljeklaus, S. & Meyer, H. E. New dimensions in the study of protein complexes using quantitative mass spectrometry. FEBS Lett. 583, 1674-1683 (2009).
7. Van Duijn, E., Barendregt, A., Synowsky, S., Versluis, C. & Heck, A. J. R. Chaperonin complexes monitored by ion mobility mass spectrometry. J. Am. Chem. Soc. 131, 1452-1459 (2009).
8. Heck, A. J. R. Native mass spectrometry: a bridge between interactomics and structural biology. Nature Methods 5, 927-933 (2008).
9. Ekinci, K. L., Huang, X. M. H. & Roukes, M. L. Ultrasensitive nanoelectromechanical mass detection. Appl. Phys. Lett. 84, 4469-4471 (2004).
10. Ilic, B. et al. Attogram detection using nanoelectromechanical oscillators. J. Appl. Phys. 95, 3694-3703 (2004).
11. Yang, Y. T., Callegari, C., Feng, X. L., Ekinci, K. L. & Roukes, M. L. Zeptogramscale nanomechanical mass sensing. Nano Lett. 6, 583-586 (2006).
12. Li, M., Tang, H. X. & Roukes, M. L. Ultra-sensitive NEMS-based cantilevers for sensing, scanned probe and very high-frequency applications. Nature Nanotech. 2, 114-120 (2007). (Pubitemid 46226371)
13. Chiu, H-Y., Hung, P., Postma, H. W. C. & Bockrath, M. Atomic-scale mass sensing using carbon nanotube resonators. Nano Lett. 8, 4342-4346 (2008).
14. Lassagne, B., Garcia-Sanchez, D., Aguasca, A. & Bachtold, A. Ultrasensitive mass sensing with a nanotube electromechanical resonator. Nano Lett. 8, 3735-3738 (2008).
15. Jensen, K., Kim, K. & Zettl, A. An atomic-resolution nanomechanical mass sensor. Nature Nanotech. 3, 533-537 (2008).
16. Naik, A. K., Hanay, M. S., Hiebert, W. K., Feng, X. L. & Roukes, M. L. Towards single-molecule nanomechanical mass spectrometry. Nature Nanotech. 4, 445-450 (2009).
17. Gil-Santos, E. et al. Nanomechanical mass sensing and stiffness spectrometry based on two-dimensional vibrations of resonant nanowires. Nature Nanotech. 5, 641-645 (2010).
18. Ekinci, K. L., Yang, Y. T. & Roukes, M. L. Ultimate limits to inertial mass sensing based upon nanoelectromechanical systems. J. Appl. Phys. 95, 2682-2689 (2004).
19. Siuzdak, G. The Expanding Role of Mass Spectrometry in Biotechnology (MCC Press, 2003).
20. Hanay, M. S. Towards Single-Molecule Nanomechanical Mass Spectrometry PhD thesis, California Insitute of Technology (2011).
21. Roukes, M. L., Naik, A. K. & Hanay, M. S. Single molecule mass spectroscopy enabled by nanoelectromechnical systems. US patent 8,227,747 (2012).
22. Dohn, S., Sandberg, R., Svendsen, W. & Boisen, A. Enhanced functionality of cantilever based mass sensors using higher modes. Appl. Phys. Lett. 86, 233501 (2005).
23. Dohn, S., Svendsen, W., Boisen, A. & Hansen, O. Mass and position determination of attached particles on cantilever based mass sensors. Rev. Sci. Instrum. 78, 103303 (2007).
24. Schmid, S., Dohn, S. & Boisen, A. Real-time particle mass spectrometry based on resonant micro strings. Sensors 10, 8092-8100 (2010).
25. Allan, D. W. Statistics of atomic frequency standards. Proc. IEEE 54, 221-230 (1966).
26. Casella, G. & Berger, R. L. Statistical Inference 2nd edn (Duxbury Press, 2001).
27. Bargatin, I., Kozinsky, I. & Roukes, M. L. Efficient electrothermal actuation of multiple modes of high-frequency nanoelectromechanical resonators. Appl. Phys. Lett. 90, 093116 (2007).
28. Mile, E. et al. In-plane nanoelectromechanical resonators based on silicon nanowire piezoresistive detection. Nanotechnology 21, 165504 (2010).
29. Caltech/CEA-LETI Alliance for Nanosystems VLSI, 200 mm (second generation) Standard NEMS process: 'CAL2?; available at http://www.nanovlsi.com.
30. Kharrat, C., Colinet, E. & Voda, A. in Sensors, 2008 IEE Conference 1135-1138 (IEEE, 2008).
31. Chiang, C. L., Hsu, M. B. & Lai, L. B. Control of nucleation and growth of gold nanoparticles in AOT/Span80/isooctane mixed reverse micelles. J. Solid State Chem. 177, 3891-3895 (2004).
32. Kim, B., Carignano, M. A., Tripp, S. L. & Wei, A. Cluster size analysis of two-dimensional order in colloidal gold nanoparticle arrays. Langmuir 20, 9360-9365 (2004).
33. Westcott, S. L., Oldenburg, S. J., Lee, T. R. & Halas, N. J. Formation and adsorption of clusters of gold nanoparticles onto functionalized silica nanoparticle surfaces. Langmuir 14, 5396-5401 (1998). (Pubitemid 128590367)
34. Tanaka, K. The origin of macromolecule ionization by laser irradiation (Nobel Lecture). Angew. Chem. Int. Ed. 42, 3860-3870 (2003).
35. Hughey, C. T., Brewer, J. W., Colosia, A. D., Rosse, W. F. & Corley, R. B. Production of IgM hexamers by normal and autoimmune B cells: implications for the physiologic role of hexameric IgM. J. Immunol. 161, 4091-4097 (1998). (Pubitemid 28468988)
36. Collins, C., Tsui, F. W. L. & Shulman, M. J. Differential activation of human and guinea pig complement by pentameric and hexameric IgM. Eur. J. Immunol. 32, 1802-1810 (2002).
37. Azuma, Y. et al. Recombinant human hexamer-dominant IgM monoclonal antibody to ganglioside GM3 for treatment of melanoma. Clin. Cancer Res. 13, 2745-2750 (2007). (Pubitemid 46788043)
38. Lindhagen-Persson, M., Brännström, K., Vestling, M., Steinitz, M. & Olofsson, A. Amyloid-b oligomer specificity mediated by the IgM isotype- implications for a specific protective mechanism exerted by endogenous autoantibodies. PLoS ONE 5, 13928 (2010).
39. Davis, A. C. & Shulman, M. J. IgM-molecular requirements for its assembly and function. Immunol. Today 10, 118-128 (1989).
40. Bacher, G. et al. Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses. J. Mass Spectrom. 36, 1038-1052 (2001). (Pubitemid 32936824)
41. Loo, J. A. et al. Electrospray ionization mass spectrometry and ion mobility analysis of the 20S proteasome complex. J. Am. Soc. Mass. Spectrom. 16, 998-1008 (2005). (Pubitemid 40828781)