Metabolic trajectory of cellular differentiation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH

Scientific Reports

Volumn 2, Issue , 2012, Pages

  Stringari, Chiara ^a   Edwards, Robert A ^b   Pate, Kira T ^c   Waterman, Marian L ^c   Donovan, Peter J ^b   Gratton, Enrico ^a  

^a University of California at Irvine   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; NICOTINAMIDE ADENINE DINUCLEOTIDE;

ANIMAL; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL TRACKING; CYTOLOGY; EPITHELIUM CELL; FLUORESCENCE MICROSCOPY; INTESTINE MUCOSA; METABOLISM; METHODOLOGY; MOUSE; SMALL INTESTINE; STEM CELL;

ANIMALS; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL TRACKING; EPITHELIAL CELLS; INTESTINAL MUCOSA; INTESTINE, SMALL; MICE; MICROSCOPY, FLUORESCENCE; NAD; STEM CELLS;

EID: 84866061103     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep00568     Document Type: Article

References (40)

1. Warburg, O. On the origin of cancer cells. Science 123, 309-314 (1956).
2. Vander Heiden, M. G., Cantley, L. C. & Thompson, C. B.Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 324(5930), 1029-33 (2009).
3. DeBerardinis, R. J., Lum, J. J., Hatzivassiliou, G.&Thompson, C. B. The Biology of Cancer: Metabolic Reprogramming Fuels Cell Growth and Proliferation. Cell Metab. 7(1), 11-20 (2008).
4. Christofk, H. R. et al. The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature 452(7184), 230-3 (2008). (Pubitemid 351380249)
5. Heikal, A. A. Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies. Biomark Med 4(2), 241-63 (2010).
6. Chance, B., Cohen,, P., Jobsis, F. & Schoener, B. Intracellular oxidation-reduction states in vivo. Science. 137, 499-508 (1962).
7. Williamson, D. H. L., Patricia & Krebs, H. A.The redox state of free nicotinamideadenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J. 103(2), 514-27 (1967).
8. Kasischke, K. A., Vishwasrao, H. D., Fisher, P. J., Zipfel, W. R. & Webb, W. W.Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis. Science 305(5680), 99-103 (2004). (Pubitemid 38869381)
9. Zipfel, W. R. et al. Live tissue intrinsic emission microscopy using multiphotonexcited native fluorescence and second harmonic generation. Proc Natl Acad Sci U S A 100(12), 7075-80 (2003). (Pubitemid 36706395)
10. Squirrell, J. M., Wokosin, D. L., White, J. G. & Bavister, B. D. Long-term twophoton fluorescence imaging of mammalian embryos without compromising viability. Nat Biotechnol. 17(8), 763-7 (1999). (Pubitemid 29365103)
11. Bird, D. K. et al, Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH. Cancer Res. 65(19), 8766-73 (2005). (Pubitemid 41377365)
12. Guo, H. W. et al. Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies. J Biomed Opt. 13(3), 050505 (2008).
13. Konig, K., Uchugonova, A. & Gorjup, E. Multiphoton fluorescence lifetime imaging of 3D-stem cell spheroids during differentiation. Microscopy Research and Technique 74(1), 9-17, 2011.
14. Stringari, C., Sierra, R., Stringari, C., Sierra, R., Donovan, P. J. & Gratton, E. Labelfree separation of human embryonic stem cells and their differentiating progenies by Phasor Fluorescence Lifetime Microscopy. J Biomed Opt. 17(4), 046012 (2012).
15. Skala, M. C. et al. In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia. Proc Natl Acad Sci U S A 104(49), 19494-9 (2007).
16. Stringari, C. et al. Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue. Proc Natl Acad Sci U S A. 108(33), 13582-7 (2011).
17. Yu, Q. & Heikal, A. A. Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level. J Photochem Photobiol B. 95(1), 46-57 (2009).
18. Banerjee, S. & Bhatt, D. K. Histochemical studies on the distribution of certain dehydrogenases in squamous cell carcinoma of cheek. Indian J Cancer 26(1), 21-30 (1989).
19. Becker, W. et al. Fluorescence lifetime imaging by time-correlated single-photon counting. Microsc Res Tech. 63(1), 58-66 (2004). (Pubitemid 37541971)
20. Colyer, R., Lee, C. & Gratton, E. A novel fluorescence lifetime imaging system that optimizes photon efficiency. Microsc Res Tech. 71(3), 201-13 (2008). (Pubitemid 351374447)
21. Digman, M. A., Caiolfa, V. R., Zamai, M. & Gratton, E. The phasor approach to fluorescence lifetime imaging analysis. Biophys J. 94(2), L14-6 (2008).
22. MacDonald, B. T., Tamai, K. & He, X. Wnt/beta-catenin signaling: components, mechanisms, and diseases. Dev Cell. 17, 9-26 (2009).
23. van de Wetering, M. et al. The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111, 241-50 (2002).
24. Reya, T. & Clevers, H. Wnt signalling in stem cells and cancer. Nature 434(7035), 843-50 (2005). (Pubitemid 40558990)
25. Klaus, A. & Birchmeier, W. Wnt signalling and its impact on development and cancer. Nat Rev Cancer. 8, 387-98 (2008). (Pubitemid 351589714)
26. van der Flier, L. G. & Clevers, H. Stem cells, self-renewal, and differentiation in the intestinal epithelium. Annu Rev Physiol. 71, 241-60 (2009).
27. Korinek, V. et al, Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nat Genet. 19(4), 379-83 (1998). (Pubitemid 28357912)
28. Najdi, R., Holcombe, R. F. & Waterman, M. L. Wnt signaling and colon carcinogenesis: beyond APC. J Carcinog. 10(5) (2011).
29. Barker, N. et al. Identification of stem cells in small intestine and colon bymarker gene Lgr5. Nature 449(7165), 1003-7 (2007). (Pubitemid 350014593)
30. Barker, N. et al. Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457(7229), 608-11 (2009).
31. Grosberg, L. E., Radosevich,A. J., Asfaha, S., Wang, T. C.&Hillman, E. M. Spectral characterization and unmixing of intrinsic contrast in intact normal and diseased gastric tissues using hyperspectral two-photon microscopy. PLoS One 6(5), e19925 (2011).
32. Ouellette, A. J. Paneth cell a-defensins in enteric innate immunity. Cell Mol Life Sci. 68(13), 2215-29 (2011).
33. Sato, T. et al. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469(7330), 415-8 (2011).
34. Batlle, E. et al. Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. Cell. 111(2), 251-63 (2002). (Pubitemid 35292445)
35. Gregorieff, A. et al. Expression pattern of Wnt signaling components in the adult intestine. Gastroenterology 129(2), 626-38 (2005). (Pubitemid 41096648)
36. vanEs, J. H. et al.Wnt signalling induces maturation of Paneth cells in intestinal crypts. Nat Cell Biol 7, 381-6 (2005).
37. de Lau W, B. N. et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476(7360), 293-7 (2011).
38. Dela Cruz, J. M., McMullen, J. D., Williams, R. M. & Zipfel, W. R. Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology. Biomed Opt Express 1, 1320-1330 (2010).
39. Liu, J. T. et al. Point-of-care pathology with miniature microscopes. Anal Cell Pathol (Amst) 34(3), 81-98 (2011).
40. Kennedy, G. T. et al. A fluorescence lifetime imaging scanning confocal endomicroscope. J Biophotonics. 3(1-2), 103-7 (2010).