Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH

Cancer Research

Volumn 65, Issue 19, 2005, Pages 8766-8773

  Bird, Damian K ^a   Yan, Long ^a,b   Vrotsos, Kristin M ^b   Eliceiri, Kevin W ^a   Vaughan, Emily M ^a   Keely, Patricia J ^a   White, John G ^a   Ramanujam, Nirmala ^b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b University of Wisconsin Madison   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE; POTASSIUM CYANIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BREAST CANCER; CELL METABOLISM; COENZYME; CONTROLLED STUDY; ELECTRON TRANSPORT; FLUORESCENCE; GROWTH CURVE; HUMAN; HUMAN CELL; IN VIVO STUDY; MITOCHONDRION; MONITORING; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; STARVATION; TECHNIQUE;

CELL LINE; CULTURE MEDIA, SERUM-FREE; HUMANS; MAMMARY GLANDS, HUMAN; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; NAD; OXIDATION-REDUCTION; POTASSIUM CYANIDE;

EID: 25444510346     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: 10.1158/0008-5472.CAN-04-3922     Document Type: Article

References (32)

1. Chance B, Cohen P, Jobsis F, Schoener B. Intracellular oxidation-reduction states in vivo. Science 1962;137:499-508.
2. Chance B, Williamson JR, Famieson D, Schoener B. Properties and kinetics of reduced pyridine nucleotide fluorescence of the isolated and in vivo rat heart. Biochem Z 1965;341:357-77.
3. Pappajohn DJ, Penneys R, Chance B. NADH spectrofluorometry of rat skin. J Appl Physiol 1972;33:684-7.
4. Avril N, Menzel M, Dose J, et al. Glucose metabolism of breast cancer assessed by 18F-FDG PET: histologic and immunohistochemical tissue analysis. J Nucl Med 2001;42:9-16.
5. Gupta PK, Majumder SK, Uppal A. Breast cancer diagnosis using N2 laser excited autofluorescence spectroscopy. Lasers Surg Med 1997;21:417-22.
6. Majumder SK, Gupta PK, Jain B, Uppal A. UV excited autofluorescence spectroscopy of human breast tissues for discriminating cancerous tissue from benign tumor and normal tissue. Lasers Life Sci 1999;8:249-64.
7. Schomacker KT, Frisoli JK, Compton CC, et al. Ultraviolet laser-induced fluorescence of colonic tissue: basic biology and diagnostic potential. Lasers Surg Med 1992;12:63-78.
8. Ramanujam N, Mitchell MF, Mahadevan A, et al. In vivo diagnosis of cervical intraepithelial neoplasia using 337-nm-excited laser-induced fluorescence. Proc Natl Acad Sci U S A 1994;91:10193-7.
9. Pradhan A, Pal P, Durocher G, et al. Steady state and time-resolved fluorescence properties of metastatic and non-metastatic malignant cells from different species. J Photochem Photobiol B 1995;31:101-12.
10. Pogue BW, Pitts JD, Mycek MA, et al. In vivo NADH fluorescence monitoring as an assay for cellular damage in photodynamic therapy. Photochem Photobiol 2001;74:817-24.
11. Konig K, Berns MW, Tromberg BJ. Time-resolved and steady-state fluorescence measurements of β-nicotinamide adenine dinucleotide-alcohol dehydrogenase complex during UVA exposure. J Photochem Photobiol B 1997;37:91-5.
12. Buchner M, Huber R, Sturchler-Pierrat C, Staufenbiel M, Riepe MW. Impaired hypoxic tolerance and altered protein binding of NADH in presymptomatic APP23 transgenic mice. Neuroscience 2002;114:285-9.
13. Wang XF, Periasamy A, Herman B. Fluorescence lifetime imaging microscopy (FLIM): instrumentation and applications. Crit Rev Annal Chem 1992;23:365-9.
14. Wakita M, Nishimura G, Tamura M. Some characteristics of the fluorescence lifetime of reduced pyridine nucleotides in isolated mitochondria, isolated hepatocytes, and perfused rat liver in situ. J Biochem (Tokyo) 1995;118:1151-60.
15. Ladokhin AS, Brand L. Evidence for an excited-state reaction contributing to NADH fluorescence. J Fluor 1995;5:99-106.
16. Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML. Fluorescence lifetime imaging of free and protein-bound NADH. Proc Natl Acad Sci U S A 1992;89:1271-5.
17. Schneckenburger H, Wagner M, Weber P, Strauss WS, Sailer R. Autofluorescence lifetime imaging of cultivated cells using a UV picosecond laser diode. J Fluor 2004;14:649-54.
18. Tadrous PJ, Siegel J, French PM, Shousha S, Lalani el N, Stamp GW. Fluorescence lifetime imaging of unstained tissues: early results in human breast cancer. J Pathol 2003;199:309-17.
19. Keely PJ, Fong AM, Zutter MM, Santoro SA. Alteration of collagen-dependent adhesion, motility, and morphogenesis by the expression of antisense α 2 integrin mRNA in mammary cells. J Cell Sci 1995;108:595-607.
20. Bird DK, Eliceiri KW, Fan CH, White JG. Simultaneous two-photon spectral and lifetime fluorescence microscopy. Appl Opt 2004;43:5173-82.
21. Press WH, Flannery BP, Teukolsky SA, Vetterling WT. Numerical recipes in C. 2nd ed. Cambridge: Cambridge University Press; 1993.
22. Becker W, Bergmann A, Biskup C, Zimmer T, Klöcker N, Benndorf K. Multi-wavelength TCSPC lifetime imaging. In: San Jose (CA): Biomedical Optics; 2002.
23. Bergmann A. SPCImage: data analysis software for fluorescence lifetime imaging microscopy. 1.6 ed. Berlin: Becker & Hickl GmbH; 2003.
24. Konig K, Riemann I. High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. J Biomed Opt 2003;8:432-9.
25. Schneckenburger H, Gschwend MH, Strauss WS, et al. Energy transfer spectroscopy for measuring mitochondrial metabolism in living cells. Photochem Photobiol 1997;66:34-41.
26. Gschwend MH, Rudel R, Strauss WS, Sailer R, Brinkmeier H, Schneckenburger H. Optical detection of mitochondrial NADH content in intact human myotubes. Cell Mol Biol (Noisy-le-grand) 2001;47 Online Pub:OL95-104.
27. Huang S, Heikal AA, Webb WW. Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. Biophys J 2002;82:2811-25.
28. Palmer GM, Keely PJ, Breslin TM, Ramanujam N. Autofluorescence spectroscopy of normal and malignant human breast cell lines. Photochem Photobiol 2003;78:462-9.
29. Berg JM, Tymoczko JL, Stryer L. Biochemistry 2001. 5th ed. New York: W H. Freeman and Company; 2002.
30. Lippitz M, Erker W, Decker H, van Holde KE, Basche T. Two-photon excitation microscopy of tryptophan-containing proteins. Proc Natl Acad Sci U S A 2002;99:2772-7.
31. Rehms A, Callis P. Two-photon fluorescence excitation spectra of aromatic amino acids. Chem Phys Lett 1993;208:276-82.
32. Vishwasrao HD, Heikal AA, Kasischke KA, Webb WW. Conformational dependence of intracellular NADH on metabolic state revealed by associated fluorescence anisotrophy. J Biol Chem 2005.