Effect of mitofusin 2 overexpression on the proliferation and apoptosis of high-glucoseinduced rat glomerular mesangial cells

Journal of Nephrology

Volumn 25, Issue 6, 2012, Pages 1023-1030

  Wan Xin, Tang ^a   Tian Lei, Cui ^a   Ben, Wang ^a   Wei Hua, Wu ^a   Ping, Fu ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Apoptosis; Diabetic nephropathy; Mitofusin 2; Proliferation; Signaling pathway

Indexed keywords

BETA CATENIN; CYCLINE; GLUCOSE; MITOFUSIN 2; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN BCL 2; PROTEIN KINASE B;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE OVEREXPRESSION; GENETIC ANALYSIS; GENETIC TRANSFECTION; INTRACELLULAR SIGNALING; MESANGIUM CELL; MITOFUSIN 2 GENE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RAT; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ADENOVIRIDAE; ANIMALS; APOPTOSIS; BLOTTING, WESTERN; CELL LINE; CELL PROLIFERATION; DIABETIC NEPHROPATHIES; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FLOW CYTOMETRY; GENETIC VECTORS; GLUCOSE; GTP PHOSPHOHYDROLASES; MESANGIAL CELLS; PHOSPHORYLATION; PROLIFERATING CELL NUCLEAR ANTIGEN; PROTO-ONCOGENE PROTEINS C-AKT; PROTO-ONCOGENE PROTEINS C-BCL-2; RATS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TIME FACTORS; TRANSFECTION; UP-REGULATION;

EID: 84865181154     PISSN: 11218428     EISSN: 17246059     Source Type: Journal    
DOI: 10.5301/jn.5000089     Document Type: Article

References (40)

1. Mason RM, Wahab NA. Extracellular matrix metabolism in diabetic nephropathy. J Am Soc Nephrol. 2003;14(5):1358-1373.
2. Haneda M, Koya D, Kikkawa R. Mesangial cell dysfunction as a pathogenesis of diabetic nephropathy. Contrib Nephrol. 2001;134(134):16-29.
3. Wolf G, Sharma K, Chen Y, Ericksen M, Ziyadeh FN. High glucose-induced proliferation in mesangial cells is reversed by autocrine TGF-beta. Kidney Int. 1992;42(3):647-656
4. Wolf G. Molecular mechanisms of diabetic mesangial cell hypertrophy: a proliferation of novel factors. J Am Soc Nephrol. 2002;13(10):2611-2613
5. Pearce JM. Apoptosis. Eur Neurol. 2008;60(2):112-116.
6. Baker AJ, Mooney A, Hughes J, Lombardi D, Johnson RJ, Savill J. Mesangial cell apoptosis: the major mechanism for resolution of glomerular hypercellularity in experimental mesangial proliferative nephritis. J Clin Invest. 1994;94(5):2105-2116.
7. Roos A, Sato T, Maier H, Daha MR, Daha MR, van Kooten C. Induction of renal cell apoptosis by antibodies and complement. Exp Nephrol. 2001;9(2):65-70.
8. Adjei AA; Alex A. Blocking oncogenic Ras signaling for cancer therapy. J Natl Cancer Inst. 2001;93(14):1062-1074.
9. Cai WJ, Wang MJ, Ju LH, Wang C, Zhu YC. Hydrogen sulfide induces human colon cancer cell proliferation: role of Akt, ERK and p21. Cell Biol Int. 2010;34(6):565-572.
10. Brown MD, Sacks DB. Protein scaffolds in MAP kinase signalling. Cell Signal. 2009;21(4):462-469.
11. Calvo M, Zhu N, Grist J, Ma Z, Loeb JA, Bennett DL. Following nerve injury neuregulin-1 drives microglial proliferation and neuropathic pain via the MEK/ERK pathway. Glia. 2011;59(4):554-568.
12. Shimamura H, Terada Y, Okado T, Tanaka H, Inoshita S, Sasaki S. The PI3-kinase-Akt pathway promotes mesangial cell survival and inhibits apoptosis in vitro via NF-kappa B and Bad. J Am Soc Nephrol. 2003;14(6):1427-1434.
13. Zhang Y, Ying B, Shi L, et al. Ghrelin inhibit cell apoptosis in pancreatic beta cell line HIT-T15 via mitogen-activated protein kinase/phosphoinositide 3-kinase pathways. Toxicology. 2007;237(1-3):194-202.
14. Won SH, Lee HJ, Jeong SJ, et al. Tanshinone IIA induces mitochondria dependent apoptosis in prostate cancer cells in association with an inhibition of phosphoinositide 3-kinase/ AKT pathway. Biol Pharm Bull. 2010;33(11):1828-1834.
15. Chen KH, Guo X, Ma D, et al. Dysregulation of HSG trig-gers vascular proliferative disorders. Nat Cell Biol. 2004;6(9): 872-883.
16. de Brito OM, Scorrano L. Mitofusin 2: a mitochondria-shaping protein with signaling roles beyond fusion. Antioxid Redox Signal. 2008;10(3):621-633.
17. Brooks C, Wei Q, Feng L, et al. Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Proc Natl Acad Sci U S A. 2007; 104(28):11649-11654.
18. Liesa M, Palacín M, Zorzano A. Mitochondrial dynamics in mammalian health and disease. Physiol Rev. 2009;89(3): 799-845.
19. Chen H, Chan DC. Mitochondrial dynamics fusion, fission, movement, and mitophagy in neurodegenerative diseases. Hum Mol Genet. 2009;18(R2):R169-R176.
20. Fyfe JC, Al-Tamimi RA, Liu J, Schäffer AA, Agarwala R, Henthorn PS. A novel mitofusin 2 mutation causes canine fetal-onset neuroaxonal dystrophy. Neurogenetics. 2011; 12(3):223-232.
21. de Brito OM, Scorrano L. Mitofusin-2 regulates mitochondrial and endoplasmic reticulum morphology and tethering: the role of Ras. Mitochondrion. 2009;9(3):222-226.
22. Chen H, Chan DC. Mitochondrial dynamics in mammals. Curr Top Dev Biol. 2004;59:119-144.
23. Fang L, Moore XL, Gao XM, Dart AM, Lim YL, Du XJ. Downregulation of mitofusin-2 expression in cardiac hypertrophy in vitro and in vivo. Life Sci. 2007;80(23):2154-2160.
24. Santel A, Fuller MT. Control of mitochondrial morphology by a human mitofusin. J Cell Sci. 2001;114(Pt 5):867-874.
25. Zamzami N, Kroemer G. Methods to measure membrane potential and permeability transition in the mitochondria during apoptosis. Methods Mol Biol. 2004;282:103-115.
26. Zamzami N, Kroemer G. Apoptosis: mitochondrial membrane permeabilization: the (w)hole story? Curr Biol. 2003;13(2):71-73.
27. Karbowski M, Lee YJ, Gaume B, et al. Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis. J Cell Biol. 2002;159(6):931-938.
28. Ingram AJ, Ly H, Thai K, Kang MJ, Scholey JW. Mesangial cell signaling cascades in response to mechanical strain and glucose. Kidney Int. 1999;56(5):1721-1728.
29. Bach D, Pich S, Soriano FX, et al. Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism: a novel regulatory mechanism altered in obesity. J Biol Chem. 2003;278(19):17190-17197.
30. Pich S, Bach D, Briones P, et al. The Charcot-Marie-Tooth type 2A gene product, Mfn2, up-regulates fuel oxidation through expression of OXPHOS system. Hum Mol Genet. 2005;14(11):1405-1415.
31. Takeuchi A, Mishina Y, Miyaishi O, Kojima E, Hasegawa T, Isobe K. Heterozygosity with respect to Zfp148 causes complete loss of fetal germ cells during mouse embryogenesis. Nat Genet. 2003;33(2):172-176.
32. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995;184(1):39-51.
33. Zhou W, Chen K-H, Cao W, et al. Mutation of the protein kinase A phosphorylation site influences the anti-proliferative activity of mitofusin 2. Atherosclerosis. 2010;211(1):216-223.
34. Cartoni R, Arnaud E, Médard J-J, et al. Expression of mitofusin 2(R94Q) in a transgenic mouse leads to Charcot-Marie- Tooth neuropathy type 2A. Brain. 2010;133(Pt 5):1460-1469.
35. Yasukawa K, Oshiumi H, Takeda M, et al. Mitofusin 2 inhibits mitochondrial antiviral signaling. Sci Signal. 2009;2:1-11.
36. Cartoni R, Léger B, Hock MB, et al. Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise. J Physiol. 2005;567(Pt 1):349-358.
37. Mingrone G, Manco M, Calvani M, Castagneto M, Naon D, Zorzano A. Could the low level of expression of the gene encoding skeletal muscle mitofusin-2 account for the metabolic inflexibility of obesity? Diabetologia. 2005;48(10):2108-2114.
38. Pawlikowska P, Gajkowska B, Orzechowski A. Mitofusin 2 (Mfn2): a key player in insulin-dependent myogenesis in vitro. Cell Tissue Res. 2007;327(3):571-581.
39. Cove-Smith A, Hendry BM. The regulation of mesangial cell proliferation. Nephron Exp Nephrol. 2008;108(4):74-79.
40. François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad. J Immunol. 2005;174(6):3633-3642.