A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine

Nature Protocols

Volumn 7, Issue 5, 2012, Pages 935-945

  Ming Hsu, Charlie Yu ^a   Uluda Ğ, Hasan ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

POLYETHYLENEIMINE;

ANIMAL; ARTICLE; CELL LINE; CULTURE TECHNIQUE; GENETIC TRANSFECTION; HUMAN; METHODOLOGY; RAT;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; HUMANS; POLYETHYLENEIMINE; RATS; TRANSFECTION;

EID: 84865173355     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.038     Document Type: Article

References (39)

1. Godbey, W.T., Wu, K.K., Mikos, A.G., Poly(ethylenimine) and its role in gene delivery. J. Control. Release 60, 149-160 (1999).
2. Akinc, A., Thomas, M., Klibanov, A.M., Langer, R., Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis. J. Gene Med. 7, 657-663 (2005).
3. Wightman, L. et al. Different behavior of branched and linear polyethylenimine for gene delivery in vitroand in vivo. J. Gene Med. 3, 362-372 (2001).
4. Rejman, J., Oberle, V., Zuhorn, I.S., Hoekstra, D., Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis. Biochem. J. 377, 159-169 (2004).
5. Gersdorff von, K. et al. The internalization route resulting in successful gene expression depends on both cell line and polyethylenimine polyplex type. Mol. Ther. 14, 745-753 (2006).
6. Izumisawa, T., Hattori, Y., Date, M., Toma, K., Maitani, Y., Cell line-dependent internalization pathways determine DNA transfection efficiency of decaarginine-PEG-lipid. Int. J. Pharm. 404, 264-270 (2011).
7. Douglas, K.L., Piccirillo, C.A., Tabrizian, M. Cell line-dependent internalization pathways and intracellular trafficking determine transfection efficiency of nanoparticle vectors. Eur. J. Pharm. Biopharm.68, 676-687 (2008).
8. Yue, Y. et al. Revisit complexation between DNA and polyethylenimine—effect of uncomplexed chains free in the solution mixture on gene transfection. J. Control. Release 155, 67-76 (2011).
9. Deng, R. et al. Revisit the complexation of PEI and DNA—how to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and structure? J. Control. Release 140, 40-46 (2009).
10. Hanzlíková, M. et al. Mechanisms of polyethylenimine-mediated DNA delivery: free carrier helps to overcome the barrier of cell-surface glycosaminoglycans. J. Gene Med. 13, 402-409 (2011).
11. Liu, Z., Zheng, M., Meng, F., Zhong, Z., Non-viral gene transfection in vitro using endosomal pH-sensitive reversibly hydrophobilized polyethylenimine. Biomaterials 32, 9109-9119 (2011).
12. Neamnark, A. et al. Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression. Mol. Pharm. 6, 1798-1815 (2009).
13. Luo, X.-H. et al. A strategy to improve serum-tolerant transfection activity of polycation vectors by surface hydroxylation. Biomaterials 32, 9925-9939 (2011).
14. Ikonen, M., Murtomäki, L., Kontturi, K. Controlled complexation of plasmid DNA with cationic polymers: effect of surfactant on the complexation and stability of the complexes. Colloids Surf B Biointerfaces 66, 77-83 (2008).
15. Mykhaylyk, O., Antequera, Y.S., Vlaskou, D., Plank, C., Generation of magnetic nonviral gene transfer agents and magnetofection in vitro. Nat. Protoc. 2, 2391-2411 (2007).
16. Laurie, K.L. et al. Cell-specific and efficient expression in mouse and human B cells by a novel hybrid immunoglobulin promoter in a lentiviral vector. Gene Ther. 14, 1623-1631 (2007).
17. Brunner, S. et al. Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus. Gene Ther. 7, 401-407 (2000).
18. Akita, H., Ito, R., Kamiya, H., Kogure, K., Harashima, H., Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression. J. Gene Med. 9, 197-207 (2007).
19. Männistö, M. et al. The role of cell cycle on polyplex-mediated gene transfer into a retinal pigment epithelial cell line. J. Gene Med. 7, 466-476 (2005).
20. Walther, W., Stein, U., Cell type specific and inducible promoters for vectors in gene therapy as an approach for cell targeting. J. Mol. Med.74, 379-392 (1996).
21. Londrigan, S.L. et al. Evaluation of promoters for driving efficient transgene expression in neonatal porcine islets. Xenotransplantation 14, 119-125 (2007).
22. Nguyen, A.T., Dow, A.C., Kupiec-Weglinski, J., Busuttil, R.W., Lipshutz, G.S., Evaluation of gene promoters for liver expression by hydrodynamic gene transfer. J. Surg. Res. 148, 60-66 (2008).
23. Zheng, C., Baum, B.J., Evaluation of viral and mammalian promoters for use in gene delivery to salivary glands. Mol. Ther. 12, 528-536 (2005).
24. Al-Dosari, M., Zhang, G., Knapp, J.E., Liu, D., Evaluation of viral and mammalian promoters for driving transgene expression in mouse liver. Biochem. Biophys. Res. Commun. 339, 673-678 (2006).
25. Pringle, I.A. et al. Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung. J. Gene Med. 9, 369-380 (2007).
26. Zhao, H. et al. Contribution of Toll-like receptor 9 signaling to the acute inflammatory response to nonviral vectors. Mol. Ther. 9, 241-248 (2004).
27. Reyes-Sandoval, A., Ertl, H.C.J., CpG methylation of a plasmid vector results in extended transgene product expression by circumventing induction of immune responses. Mol. Ther. 9, 249-261 (2004).
28. Hodges, B.L., Taylor, K.M., Joseph, M.F., Bourgeois, S.A., Scheule, R.K., Long-term transgene expression from plasmid DNA gene therapy vectors is negatively affected by CpG dinucleotides. Mol. Ther. 10, 269-278 (2004).
29. Chen, Z.Y., He, C.Y., Meuse, L., Kay, M.A., Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivo. Gene Ther. 11, 856-864 (2004).
30. Chen, Z.Y., He, C.-Y., Ehrhardt, A., Kay, M.A., Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivo. Mol. Ther. 8, 495-500 (2003).
31. Huang, M. et al. Novel minicircle vector for gene therapy in murine myocardial infarction. Circulation 120, S230-S237 (2009).
32. Jia, F. et al. A nonviral minicircle vector for deriving human iPS cells. Nat. Methods 7, 197-199 (2010).
33. Hsu, C.Y.M., Hendzel, M., Uludag˘, H., Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell. J. Gene Med. 13, 46-59 (2011).
34. Subrizi, A. et al. Optimized transfection protocol for efficient in vitronon-viral polymeric gene delivery to human retinal pigment epithelial cells (ARPE-19). Protoc. Exchange doi:10.1038/nprot.2009.78 (2009).
35. Farrell, L.-L. et al. A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmid DNA to bone marrow stromal cells (BMSC). Eur. J. Pharm. Biopharm. 65, 388-397 (2007).
36. Hsu, C.Y.M., Uludağ, H., Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers. BMC Biotechnol. 8, 23 (2008).
37. Wang, J. et al. Deep dermal fibroblasts contribute to hypertrophic scarring. Lab. Invest. 88, 1278-1290 (2008).
38. Jacobsen, L.B., Calvin, S.A., Colvin, K.E., Wright, M., FuGENE 6 transfection reagent: the gentle power. Methods 33, 104-112 (2004).
39. Utsuno, K., Uludag˘, H., Thermodynamics of polyethylenimine-DNA binding and DNA condensation. Biophys. J. 99, 201-207 (2010).