Revisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection

Journal of Controlled Release

Volumn 155, Issue 1, 2011, Pages 67-76

  Yue, Yanan ^a   Jin, Fan ^a   Deng, Rui ^a   Cai, Jinge ^a   Chen, Yangchao ^a   Lin, Marie C M ^b   Kung, Hsiang Fu ^a   Wu, Chi ^a,c  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

^c UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Cellular internalization; DNA condensation; Free polyethylenimine; Gene delivery; Laser light scattering

Indexed keywords

CELLULAR INTERNALIZATION; DNA CONDENSATION; GENE DELIVERY; LASER LIGHT SCATTERING; POLYETHYLENIMINES;

CONDENSATION; ELECTROPHORESIS; FLOW CYTOMETRY; GENE THERAPY; GENE TRANSFER; LIGHT SCATTERING; SALINE WATER;

DNA;

DNA; NITROGEN; PHOSPHATE; POLYETHYLENEIMINE;

ARTICLE; CELL MIGRATION; COMPLEX FORMATION; CONTROLLED STUDY; CYTOTOXICITY; DNA BINDING; FLOW CYTOMETRY; GEL ELECTROPHORESIS; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO GENE TRANSFER; INTERNALIZATION; LIGHT SCATTERING; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL;

CELL LINE; DNA; FLOW CYTOMETRY; HUMANS; LASERS; MODELS, MOLECULAR; POLYETHYLENEIMINE; SCATTERING, RADIATION; TRANSFECTION;

EID: 79953839673     PISSN: 01683659     EISSN: 18734995     Source Type: Journal    
DOI: 10.1016/j.jconrel.2010.10.028     Document Type: Article

References (54)

1. E. Check Gene therapy: a tragic setback Nature 420 6912 2002 116 118
2. S.E. Raper, N. Chirmule, F.S. Lee, N.A. Wivel, A. Bagg, G.P. Gao, J.M. Wilson, and M.L. Batshaw Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer Mol. Genet. Metab. 80 1-2 2003 148 158 (Pubitemid 37272241)
3. S. Hacien-Bey-Abina LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1 Science 302 5644 2003 415 419
4. D.W. Pack, A.S. Hoffman, S. Pun, and P.S. Stayton Design and development of polymers for gene delivery Nat. Rev. Drug Discov. 4 7 2005 581 593 (Pubitemid 40975711)
5. S.D. Li, and L. Huang Non-viral is superior to viral gene delivery J. Control. Release. 123 3 2007 181 183 (Pubitemid 350007827)
6. M.A. Mintzer, and E.E. Simanek Nonviral vectors for gene delivery Chem. Rev. 109 2 2009 259 302
7. O. Boussif, F. Lezoualch, M.A. Zanta, M.D. Mergny, D. Scherman, B. Demeneix, and J.P. Behr A versatile vector for gene and oligonucleotide transfer into cells in culture and in-vivo - polyethylenimine Proc. Natl. Acad. Sci. U. S. A. 92 16 1995 7297 7301
8. U. Lungwitz, M. Breunig, T. Blunk, and A. Gopferich Polyethylenimine- based non-viral gene delivery systems Eur. J. Pharm. Biopharm. 60 2 2005 247 266 (Pubitemid 40804565)
9. M. Neu, D. Fischer, and T. Kissel Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives J. Gene Med. 7 8 2005 992 1009 (Pubitemid 41195062)
10. M. Breunig, U. Lungwitz, R. Liebl, and A. Goepferich Breaking up the correlation between efficacy and toxicity for nonviral gene delivery Proc. Natl. Acad. Sci. U. S. A. 104 36 2007 14454 14459 (Pubitemid 350003282)
11. R. Deng, Y. Yue, F. Jin, Y. Chen, H. Kung, M. Lin, and C. Wu Revisit the complexation of PEI and DNA - How to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and strucuture? J. Control. Release. 140 1 2009 40 46
12. M. Ogris, S. Brunner, S. Schuller, R. Kircheis, and E. Wagner PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery Gene Ther. 6 4 1999 595 605 (Pubitemid 29192668)
13. H. Cheng, J.L. Zhu, X. Zeng, Y. Jing, X.Z. Zhang, and R.X. Zhuo Targeted gene delivery mediated by folate-polyethylenimine-block-poly(ethylene glycol) with receptor selectivity Bioconjugate Chem. 20 3 2009 481 487
14. M.A. Zanta, O. Boussif, A. Adib, and J.P. Behr In vitro gene delivery to hepatocytes with galactosylated polyethylenimine Bioconjugate Chem. 8 6 1997 839 844 (Pubitemid 27526411)
15. S.S. Diebold, P. Kursa, E. Wagner, M. Cotten, and M. Zenke Mannose polyethylenimine conjugates for targeted DNA delivery into dendritic cells J. Biol. Chem. 274 27 1999 19087 19094
16. H. Pollard, J.S. Remy, G. Loussouarn, S. Demolombe, J.P. Behr, and D. Escande Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells J. Biol. Chem. 273 13 1998 7507 7511 (Pubitemid 28152775)
17. W.T. Godbey, K.K. Wu, and A.G. Mikos Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery Proc. Natl. Acad. Sci. U. S. A. 96 9 1999 5177 5181 (Pubitemid 29214556)
18. T. Bieber, W. Meissner, S. Kostin, A. Niemann, and H.P. Elsasser Intracellular route and transcriptional competence of polyethylenimine-DNA complexes J. Control. Release. 82 2-3 2002 441 454
19. N.D. Sonawane, F.C. Szoka, and A.S. Verkman Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes J. Biol. Chem. 278 45 2003 44826 44831 (Pubitemid 37377241)
20. J. Suh, D. Wirtz, and J. Hanes Efficient active transport of gene nanocarriers to the cell nucleus Proc. Natl. Acad. Sci. U. S. A. 100 7 2003 3878 3882 (Pubitemid 36418125)
21. A. Akinc, M. Thomas, A.M. Klibanov, and R. Langer Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis J. Gene Med. 7 5 2005 657 663 (Pubitemid 40744532)
22. R.P. Kulkarni, D.D. Wu, M.E. Davis, and S.E. Fraser Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy Proc. Natl. Acad. Sci. U. S. A. 102 21 2005 7523 7528 (Pubitemid 40741004)
23. K. de Bruin, N. Ruthardt, K. von Gersdorff, R. Bausinger, E. Wagner, M. Ogris, and C. Brauchle Cellular dynamics of EGF receptor-targeted synthetic viruses Mol. Ther. 15 7 2007 1297 1305 (Pubitemid 46965326)
24. K.G. de Bruin, C. Fella, M. Ogris, E. Wagner, N. Ruthardt, and C. Brauchle Dynamics of photoinduced endosomal release of polyplexes J. Control. Release. 130 2 2008 175 182
25. N.P. Gabrielson, and D.W. Pack Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells J. Control. Release. 136 1 2009 54 61
26. Y.Y. Won, R. Sharma, and S.F. Konieczny Missing pieces in understanding the intracellular trafficking of polycation/DNA complexes J. Control. Release. 139 2 2009 88 93
27. H. Lee, I.K. Kim, and T.G. Park Intracellular trafficking and unpacking of siRNA/quantum Dot-PEI complexes modified with and without cell penetrating peptide: confocal and flow cytometric FRET analysis Bioconjugate Chem 21 2 2010 289 295
28. E. Wagner, M. Cotten, R. Foisner, and M.L. Birnstiel Transferrin polycation DNA complexes - the effect of polycations on the structure of the complex and DNA delivery to cells Proc. Natl. Acad. Sci. U. S. A. 88 10 1991 4255 4259 (Pubitemid 21914518)
29. W.T. Godbey, K.K. Wu, and A.G. Mikos Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle J. Biomed. Mater. Res. 45 3 1999 268 275 (Pubitemid 29165943)
30. L. Wightman, R. Kircheis, V. Rossler, S. Carotta, R. Ruzicka, M. Kursa, and E. Wagner Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo J. Gene Med. 3 4 2001 362 372 (Pubitemid 33691776)
31. K. Kunath, A. von Harpe, D. Fischer, H. Peterson, U. Bickel, K. Voigt, and T. Kissel Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine J. Control. Release. 89 1 2003 113 125 (Pubitemid 36412116)
32. I. Honore, S. Grosse, N. Frison, F. Favatier, M. Monsigny, and I. Fajac Transcription of plasmid DNA: influence of plasmid DNA/polyethylenimine complex formation J. Control. Release. 107 3 2005 537 546 (Pubitemid 41393360)
33. S.H. Pun, N.C. Bellocq, A.J. Liu, G. Jensen, T. Machemer, E. Quijano, T. Schluep, S.F. Wen, H. Engler, J. Heidel, and M.E. Davis Cyclodextrin-modified polyethylenimine polymers for gene delivery Bioconjugate Chem. 15 4 2004 831 840 (Pubitemid 38981958)
34. T. Ooya, H.S. Choi, A. Yamashita, N. Yui, Y. Sugaya, A. Kano, A. Maruyama, H. Akita, R. Ito, K. Kogure, and H. Harashima Biocleavable polyrotaxane - Plasmid DNA polyplex for enhanced gene delivery J. Am. Chem. Soc. 128 12 2006 3852 3853
35. H.Y. Tian, W. Xiong, J.Z. Wei, Y. Wang, X.S. Chen, X.B. Jing, and Q.Y. Zhu Gene transfection of hyperbranched PEI grafted by hydrophobic amino acid segment PBLG Biomaterials 28 18 2007 2899 2907 (Pubitemid 46482665)
36. Y.X. Sun, W. Xiao, S.X. Cheng, X.Z. Zhang, and R.X. Zhuo Synthesis of (Dex-HMDI)-g-PEIs as effective and low cytotoxic nonviral gene vectors J. Control. Release. 128 2 2008 171 178
37. B. Liang, M.L. He, C.Y. Chan, Y.C. Chen, X.P. Li, Y. Li, D.X. Zheng, M.C. Lin, H.F. Kung, X.T. Shuai, and Y. Peng The use of folate-PEG-grafted- hybranched-PEI nonviral vector for the inhibition of glioma growth in the rat Biomaterials 30 23-24 2009 4014 4020
38. Bloomfield V.A. DNA condensation by multivalent cations Biopolymers 44 3 1997 269 282
39. E. Lai, and J.H. van Zanten Monitoring DNA/poly-l-lysine polyplex formation with time-resolved multiangle laser light scattering Biophys. J. 80 2 2001 864 873 (Pubitemid 32128337)
40. E. Vuorimaa, A. Urtti, R. Seppanen, H. Lemmetyinen, and M. Yliperttula Time-resolved fluorescence spectroscopy reveals functional differences of cationic polymer-DNA complexes J. Am. Chem. Soc. 130 35 2008 11695 11700
41. J. Ziebarth, and Y.M. Wang Molecular dynamics simulations of DNA-polycation complex formation Biophys. J. 97 7 2009 1971 1983
42. J.P. Behr The proton sponge: a trick to enter cells the viruses did not exploit Chimia 51 1-2 1997 34 36
43. J.P. Clamme, J. Azoulay, and Y. Mely Monitoring of the formation and dissociation of polyethylenimine/DNA complexes by two photon fluorescence correlation spectroscopy Biophys. J. 84 3 2003 1960 1968 (Pubitemid 36322958)
44. S. Boeckle, K. von Gersdorff, S. van der Piepen, C. Culmsee, E. Wagner, and M. Ogris Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer J. Gene Med. 6 10 2004 1102 1111 (Pubitemid 40228122)
45. J. Fahrmeir, M. Gunther, N. Tietze, E. Wagner, and M. Ogris Electrophoretic purification of tumor-targeted polyethylenimine-based polyplexes reduces toxic side effects in vivo J. Control. Release. 122 3 2007 236 245 (Pubitemid 47404673)
46. J.P. Clamme, G. Krishnamoorthy, and Y. Mely Intracellular dynamics of the gene delivery vehicle polyethylenimine during transfection: investigation by two-photon fluorescence correlation spectroscopy Biochim. Biophys. Acta, Biomembr. 1617 1-2 2003 52 61 (Pubitemid 37456712)
47. J.M. Saul, C.H.K. Wang, C.P. Ng, and S.H. Pun Multilayer nanocomplexes of polymer and DNA exhibit enhanced gene delivery Adv. Mater. 20 6 2008 19 25 (Pubitemid 351143287)
48. B. Berne, and R. Pecora Dynamic Light Scattering 1976 Plenum Press New York
49. B. Chu Laser Light Scattering second ed. 1991 Academic Press New York
50. R.J. Hunter Foundations of Colloid Science second ed. 2000 Oxford Press Oxford
51. M.L. Forrest, and D.W. Pack On the kinetics of polyplex endocytic trafficking: implications for gene delivery vector design Mol. Ther. 6 1 2002 57 66 (Pubitemid 36124271)
52. M. Breunig, U. Lungwitz, R. Liebl, C. Fontanari, J. Klar, A. Kurtz, T. Blunk, and A. Goepferich Gene delivery with low molecular weight linear polyethytenimines J. Gene Med. 7 10 2005 1287 1298 (Pubitemid 41555435)
53. C. Wu, and K.Q. Xia Incorporation of a differential refractometer into a laser light-scattering spectrometer Rev. Sci. Instrum. 65 3 1994 587 590
54. J. Rejman, A. Bragonzi, and M. Conese Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes Mol. Ther. 12 3 2005 468 474 (Pubitemid 41150132)