Molecular cloning and characterization of procirsin, an active aspartic protease precursor from Cirsium vulgare (Asteraceae)

Phytochemistry

Volumn 81, Issue , 2012, Pages 7-18

  Lufrano, Daniela ^a   Faro, Rosário ^b   Castanheira, Pedro ^c   Parisi, Gustavo ^d   Veríssimo, Paula ^b,e   Vairo Cavalli, Sandra ^a   Simões, Isaura ^b,c   Faro, Carlos ^b,c,e  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b UNIVERSITY OF COIMBRA   (Portugal)

^c Biocant Biotechnology Innovation Center   (Portugal)

^d UNIVERSIDAD NACIONAL DE QUILMES   (Argentina)

^e UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

Aspartic proteinase; Asteraceae; Bull thistle; Cirsin; Cirsium vulgare; Milk clotting activity; Pepsin like protease; Procirsin

Indexed keywords

ASPARTIC PROTEINASE; CASEIN; COMPLEMENTARY DNA; CYPROSIN; PROCIRSIN; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; CIRSIUM; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; FLOWER; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PH; PROTEIN DEGRADATION; SEQUENCE HOMOLOGY;

ASPARTIC ACID ENDOPEPTIDASES; CASEINS; CIRSIUM; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ENZYME ACTIVATION; ENZYME ASSAYS; ESCHERICHIA COLI; FLOWERS; HYDROGEN-ION CONCENTRATION; PLANT PROTEINS; PROTEOLYSIS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ASTERACEAE; CIRSIUM; CIRSIUM VULGARE; ESCHERICHIA COLI; WITHANIA COAGULANS;

EID: 84864746972     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2012.05.028     Document Type: Article

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