Characterization of papain-like isoenzymes from latex of Asclepias curassavica by molecular biology validated by proteomic approach

Biochimie

Volumn 91, Issue 11-12, 2009, Pages 1457-1464

  Obregón, Walter D ^a   Liggieri, Constanza S ^a   Trejo, Sebastian A ^b   Avilés, Francesc X ^b   Vairo Cavalli, Sandra E ^a   Priolo, Nora S ^a  

^a FACULTAD DE CIENCIAS EXACTAS   (Argentina)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Asclepias curassavica; Cysteine peptidase; Papain like protease; Peptide mass fingerprint; Plant latex

Indexed keywords

ASCLEPAIN; COMPLEMENTARY DNA; ISOENZYME; LATEX; PAPAIN; PEPTIDASE; POLYPEPTIDE; PRIMER DNA; RNA; TRYPSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ASCLEPIAS; ASCLEPIAS CURASSAVICA; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; GENETIC CONSERVATION; INTERMETHOD COMPARISON; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEOMICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; VALIDATION PROCESS;

ASCLEPIAS; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEASES; CYSTEINE PROTEINASE INHIBITORS; HYDROLYSIS; ISOENZYMES; LATEX; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

APOCYNACEAE; ASCLEPIAS; ASCLEPIAS CURASSAVICA; ASCLEPIAS FRUTICOSA;

EID: 73049092070     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2009.07.017     Document Type: Article

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