Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L.

Plant Molecular Biology

Volumn 45, Issue 5, 2001, Pages 529-539

  Vieira, Margarida ^a   Pissarra, José ^b   Veríssimo, Paula ^a   Castanheira, Pedro ^a   Costa, Yael ^b   Pires, Euclides ^a   Faro, Carlos ^a  

^a UNIVERSITY OF COIMBRA   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

Author keywords

Aspartic proteinase; Cardosin B; Pistil development; Transmitting tissue

Indexed keywords

ANTIBODY; ASPARTIC PROTEINASE; CARDOSIN; CHEESE; ENZYME LOCALIZATION; GENETIC REGULATION; INFLORESCENCE; MESSENGER RNA; PISTIL; PLANT DEVELOPMENT; PLANT TISSUE; PRODUCTION; SEQUENCE HOMOLOGY; TISSUE DISTRIBUTION;

ANTIBODIES; DNA; ENZYMES; PYROLYSIS; TISSUE;

IMMUNOCYTOCHEMISTRY;

CLONING;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ASPARTIC ENDOPEPTIDASES; ASTERACEAE; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION, PLANT; IMMUNOHISTOCHEMISTRY; MOLECULAR SEQUENCE DATA; PLANT COMPONENTS; PLANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE ALIGNMENT;

EID: 0034982756     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010675015318     Document Type: Article

References (42)

1. Rice aspartic proteinase, orysazin, expressed during seed ripening and germination, has a gene organization distinct from those of animal and microbial aspartic proteinases
2. The aspartic proteinases. A historical overview
3. Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration
4. Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin
5. The glycosylation of the aspartic proteinases from barley (Hordeum vulgare L.) and cardoon (Cynara cardunculus L.)
6. Aspartic proteinase genes in the Brassicaceae Arabidopsis thaliana and Brassica napus
7. Cloning and characterization of a cDNA encoding cardosin A, an RGD-containing plant aspartic proteinase
8. A promoter directing high-level expression in pistils of transgenic plants
9. Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp containing aspartic proteinase from the flowers of Cynara cardunculus L.
10. Transport and activation of the vacuolar aspartic proteinase phytepsin in barley (Hordeum vulgare L.)
11. Comparative modelling of barley-grain aspartic proteinases: A structural rationale for observed hydrolytic specificity
12. Crystal structure of plant aspartic proteinase prophytepsin: Inactivation and vacuolar targeting
13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
14. Saposin fold revealed by the NMR structure of NK-lysin
15. Human aspartic protease memapsin 2 cleaves the β-secretase site of β-amyloid precursor protein
16. Self-incompatibility gene products of Nicotiana alata are ribonucleases
17. Plant aspartic proteinases: Enzymes on the way to a function
18. The aspartic proteinase is expressed in Arabidopsis thaliana seeds and localized in the protein bodies
19. Pepstatin-insensitive carboxyl proteinases from prokaryotes. Catalytic residues and substrate specificities
20. Saposin proteins: Structure, function and role in human lysosomal storage disorders
21. Identification of senescence-associated genes from daylily petals
22. Isolation of total poly(A) and polysomal RNA from plant tissues
23. Action on bovine s1-casein of cardosins A and B, aspartic proteinases from the flowers of the cardoon Cynara cardunculus L.
24. Cardosin A, an abundant aspartic proteinase, accumulates in protein storage vacuoles in the stigmatic papillae of Cynara cardunculus L.
25. Identification and proteolytic processing of procardosin A
26. Degradation of tomato pathogenesis-related proteins by an endogenous 37 kDa aspartyl endoproteinase
27. Degradation of tobacco pathogenesis-related proteins
28. Primary structure of a barley-grain aspartic proteinase. A plant aspartic proteinase resembling mammalian cathepsin D
29. Phytepsin, a barley vacuolar aspartic proteinase, is highly expressed during autolysis of developing tracheary elements and sieve cells
30. The aspartic proteinase of barley is a vacuolar enzyme that processes probarley lectin in vitro
31. Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein
32. Purification and cloning of amyloid precursor protein β-secretase from human brain
33. Structural evidence for gene duplication in the evolution of the acid proteases
34. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic proteinase BACE
35. Purification, characterization and partial amino acid sequence of two novel aspartic proteinases from fresh flowers of Cynara cardunculus L.
36. The most abundant soluble basic protein of the stylar transmitting tract in potato (Solanum tuberosum L.) is an endochitinase
37. Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus)
38. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity