Feasibility of Pisum sativum as an expression system for pharmaceuticals

Transgenic Research

Volumn 21, Issue 4, 2012, Pages 715-724

  Mikschofsky, Heike ^a   Broer, Inge ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Molecular farming; Pea derived pharmaceuticals; Plant derived vaccines; Transformation; Transgenic peas

Indexed keywords

EDIBLE VACCINE; RECOMBINANT PROTEIN;

BIOSYNTHESIS; GENE EXPRESSION; GENETICS; HUMAN; MOLECULAR FARMING; PEA; PLANT SEED; REVIEW; TRANSGENIC PLANT;

GENE EXPRESSION; HUMANS; MOLECULAR FARMING; PEAS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SEEDS; VACCINES, EDIBLE;

PISUM SATIVUM;

EID: 84864046231     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-011-9573-z     Document Type: Review

References (74)

1. Amanullah A, Otero JM, Mikola M, Hsu A, Zhang JY, Aunins J, Schreyer HB, Hope JA, Russo AP (2010) Novel micro-bioreactor high throughput technology for cell culture process development: reproducibility and scalability assessment of fed-batch CHO cultures. Biotechnol Bioeng 106(1): 57-67. doi: 10. 1002/Bit. 22664.
2. Arora DGAK, Paliwal SR, Khurana B, Vyas SP (2010) Oral mucosal immunization: recent advancement and future prospects. Curr Immunol Rev 6(3): 234-259.
3. Bean SJ, Gooding PS, Mullineaux PM, Davies DR (1997) A simple system for pea transformation. Plant Cell Rep 16(8): 513-519.
4. Bickel-Sandkötter S (2001) Nutzpflanzen und ihre Inhaltsstoffe. UTB für Wissenschaft, Stuttgart.
5. Boothe JG, Nykiforuk CL, Kuhlman PA, Whelan H, Pollock WBRCS, Yuan S, Kumar R, Murray EW, Visser F, Martens K, Wu J, Pollock E, Given B, Szarka S, Zaplachinski S, Harry I, Keon R, Moloney MM (2009) Analytical characterization, safety and clinical bioequivalence of recombinant human insulin from transgenic plants. American Diabetes Association 69th Scientific Sessions Abstract 5-LB. American Diabetes Association, Alexandria.
6. Boothe J, Nykiforuk C, Shen Y, Zaplachinski S, Szarka S, Kuhlman P, Murray E, Morck D, Moloney MM (2010) Seed-based expression systems for plant molecular farming. Plant Biotechnol J 8(5): 588-606.
7. Bouwman AJ (1992) Maintenance breeding and multiplication of pea and faba bean cultivars-maintenance of protein peas (Pisum sativum) and field beans (Vicia faba). Euphytica 61(3): 213-215.
8. Boyhan D, Daniell H (2011) Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide. Plant Biotechnol J 9(5): 585-598. doi: 10. 1111/j. 1467-7652. 2010. 00582. x.
9. Castanon S, Martin-Alonso JM, Marin MS, Boga JA, Alonso P, Parra F, Ordas RJ (2002) The effect of the promoter on expression of VP60 gene from rabbit hemorrhagic disease virus in potato plants. Plant Sci 162(1): 87-95.
10. Conner AJ, Dale PJ (1996) Reconsideration of pollen dispersal data from field trials of transgenic potatoes. Theor Appl Genet 92(5): 505-508.
11. Conrad U, Fiedler U (1998) Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol Biol 38(1-2): 101-109.
12. Damgaard C, Loeschcke V (1994) Inbreeding depression and dominance suppression competition after inbreeding in rapeseed (Brassica-Napus). Theor Appl Genet 88(3-4): 321-323.
13. Daniell H, Singh ND, Mason H, Streatfield SJ (2009) Plant-made vaccine antigens and biopharmaceuticals. Trends Plant Sci 14(12): 669-679. doi: 10. 1016/j. tplants. 2009. 09. 009.
14. Davies DR, Hamilton J, Mullineaux P (1993) Transformation of peas. Plant Cell Rep 12(3): 180-183.
15. De la Rosa GP, Monroy-Garcia A, Mora-Garcia MD, Pena CG, Hernandez-Montes J, Weiss-Steider B, Lim MAG (2009) An HPV 16 L1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T-cell activity in mice. Virol J 6: 2. doi: 10. 1186/1743-422x-6-2.
16. Dekathen A, Jacobsen HJ (1990) Agrobacterium tumefaciens-mediated transformation of Pisum sativum l using binary and cointegrate vectors. Plant Cell Rep 9(5): 276-279.
17. Edwards JW, Lamkey KR (2002) Quantitative genetics of inbreeding in a synthetic maize population. Crop Sci 42(4): 1094-1104.
18. European Food Safety Authority (EFSA) (2004) Opinion of the scientific panel on genetically modified organisms on the use of antibiotic resistance genes as marker genes in genetically modified plants. The EFSA Journal, 48, 1-18. http://www. efsa. europa. eu/en/efsajournal/doc/48. pdf.
19. Fernandez-Quintela A, Macarulla MT, Del Barrio AS, Martinez JA (1997) Composition and functional properties of protein isolates obtained from commercial legumes grown in northern spain. Plants Food Hum Nutr 51: 331-342.
20. Grant J, Cooper P (2006) Peas (Pisum sativum L.). Methods Mol Biol 343: 337-345.
21. Grant JE, Cooper PA, McAra AE, Frew TJ (1995) Transformation of peas (Pisum sativum L.) using immature cotyledons. Plant Cell Rep 15: 254-258.
22. Grant JE, Thomson LMJ, Pither-Joyce MD, Dale TM, Cooper PA (2003) Influence of Agrobacterium tumefaciens strain on the production of transgenic peas (Pisum sativum L.). Plant Cell Rep 21(12): 1207-1210.
23. Hellwig S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. Nat Biotechnol 22(11): 1415-1422. doi: 10. 1038/Nbt1027.
24. Holmgren J, Czerkinsky C (2005) Mucosal immunity and vaccines. Nat Med 11[4 Suppl]: S45-53. doi: 10. 1038/nm1213.
25. Hood EE, Love R, Lane J, Bray J, Clough R, Pappu K, Drees C, Hood KR, Yoon S, Ahmad A, Howard JA (2007) Subcellular targeting is a key condition for high-level accumulation of cellulase protein in transgenic maize seed. Plant Biotechnol J 5(6): 709-719.
26. Huang N (2004) High-level protein expression system uses self pollinating crops as hosts. BioProcess Int 2: 54-59.
27. Kim TG, Yang MS (2010) Current trends in edible vaccine development using transgenic plants. Biotechnol Bioproc E 15(1): 61-65. doi: 10. 1007/s12257-009-3084-2.
28. Krejci P, Matuskova P, Hanacek P, Reinohl V, Prochazka S (2007) The transformation of pea (Pisum sativum L.): applicable methods of Agrobacterium tumefaciens-mediated gene transfer. Acta Physiol Plant 29(2): 157-163.
29. Lau OS, Sun SSM (2009) Plant seeds as bioreactors for recombinant protein production. Biotechnol Adv 27(6): 1015-1022.
30. Lavelle EC, O'Hagan DT (2006) Delivery systems and adjuvants for oral vaccines. Expert Opin Drug Deliv 3(6): 747-762. doi: 10. 1517/17425247. 3. 6. 747.
31. Lindermayer H, Propstmeier G, Straub K (2002) Fütterungsberater Schwein: Ferkel, Zuchtschweine, Mastschweine. München: BLV Verlagsgesellschaft mbH.
32. Lou XM, Yao QH, Zhang Z, Peng RH, Xiong AS, Wang HK (2007) Expression of the human hepatitis B virus large surface antigen gene in transgenic tomato plants. Clin Vaccine Immunol 14(4): 464-469. doi: 10. 1128/Cvi. 00321-06.
33. Lugade AA, Kalathil S, Heald JL, Thanavala Y (2010) Transgenic plant-based oral vaccines. Immunol Invest 39(4-5): 468-482. doi: 10. 3109/08820131003622643.
34. Mallory-Smith C, Zapiola M (2008) Gene flow from glyphosate-resistant crops. Pest Manag Sci 64(4): 428-440.
35. Malmberg RL (1979) Regeneration of whole plants from callus-culture of diverse genetic lines of Pisum sativum L. Planta 146(2): 243-244.
36. Mason HS, Ball JM, Shi JJ, Jiang X, Estes MK, Arntzen CJ (1996) Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci USA 93(11): 5335-5340.
37. McPhee KE (2008) Transgenic legume grains and forage grasses. In: Kole C, Hall TC (eds) Compendium of transgenic crop plants. Blackwell, Oxford.
38. Mestecky J, Nguyen H, Czerkinsky C, Kiyono H (2008) Oral immunization: an update. Curr Opin Gastroen 24(6): 713-719. doi: 10. 1097/Mog. 0b013e32830d58be.
39. Mikschofsky H, Konig P, Keil GM, Hammer M, Schirrmeier H, Broer I (2009a) Cholera toxin B (CTB) is functional as an adjuvant for cytoplasmatic proteins if directed to the endoplasmatic reticulum (ER), but not to the cytoplasm of plants. Plant Sci 177(1): 35-42.
40. Mikschofsky H, Schirrmeier H, Keil GM, Lange B, Polowick PL, Keller W, Broer I (2009b) Pea-derived vaccines demonstrate high immunogenicity and protection in rabbits against rabbit haemorrhagic disease virus. Plant Biotechnol J 7(6): 537-549.
41. Mikschofsky H, Heilmann E, Schmidtke J, Schmidt K, Meyer U, Leinweber P, Broer I (2011) Greenhouse and field cultivations of antigen-expressing potatoes focusing on the variability in plant constituents and antigen expression. Plant Mol Biol 76(1-2): 131-144. doi: 10. 1007/s11103-011-9774-0.
42. Nadolska-Orczyk A, Orczyk W (2000) Study of the factors influencing Agrobacterium-mediated transformation of pea (Pisum sativum L.). Mol Breed 6(2): 185-194.
43. Perrin Y, Vaquero C, Gerrard I, Sack M, Drossard J, Stoger E, Christou P, Fischer R (2000) Transgenic pea seeds as bioreactors for the production of a single-chain Fv fragment (scFV) antibody used in cancer diagnosis and therapy. Mol Breed 6(4): 345-352.
44. Pniewski T, Wachowiak J, Kapusta J, Legocki AB (2003) Organogenesis and long-term micropropagation of Polish pea cultivars. Acta Soc Bot Pol 72(4): 295-302.
45. Polowick PL, Quandt J, Mahon JD (2000) The ability of pea transformation technology to transfer genes into peas adapted to western Canadian growing conditions. Plant Sci 153(2): 161-170.
46. Polowick PL, Vandenberg A, Mahon JD (2002) Field assessment of outcrossing from transgenic pea (Pisum sativum L.) plants. Transgenic Res 11(5): 515-519.
47. Pribylova R, Pavlik I, Bartos A (2006) Genetically modified potato plants in nutrition and prevention of diseases in humans and animals: a review. Vet Med-Czech 51(5): 212-223.
48. Puonti Kaerlas J, Ottosson A, Eriksson T (1992) Survival and growth of pea protoplasts after transformation by electroporation. Plant Cell Tiss Organ Cult 30(2): 141-148.
49. Puonti-Kaerlas J, Stabel P, Eriksson T (1989) Transformation of pea (Pisum sativum L.) by Agrobacterium tumefaciens. Plant Cell Rep 8(6): 321-324.
50. Puonti-Kaerlas J, Eriksson T, Engstrom P (1990) Production of transgenic pea (Pisum sativum) plants by Agrobacterium tumefaciens-mediated gene transfer. Theor Appl Genet 80(2): 246-252.
51. Ramessar K, Sabalza M, Capell T, Christou P (2008) Maize plants: an ideal production platform for effective and safe molecular pharming. Plant Sci 174(4): 409-419. doi: 10. 1016/j. plantsci. 2008. 02. 002.
52. Richter LJ, Thanavala Y, Arntzen CJ, Mason HS (2000) Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nat Biotechnol 18(11): 1167-1171.
53. Roy F, Boye JI, Simpson BK (2010) Bioactive proteins and peptides in pulse crops: pea, chickpea and lentil. Food Res Int 43(2): 432-442.
54. Ruhlman T, Ahangari R, Devine A, Samsam M, Daniell H (2007) Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplasts-oral administration protects against development of insulitis in non-obese diabetic mice. Plant Biotechnol J 5(4): 495-510. doi: 10. 1111/j. 1467-7652. 2007. 00259. x.
55. Rybicki EP (2010) Plant-made vaccines for humans and animals. Plant Biotechnol J 8(5): 620-637.
56. Saalbach I, Giersberg M, Conrad U (2001) High-level expression of a single-chain Fv fragment (scFv) antibody in transgenic pea seeds. J Plant Physiol 158(4): 529-533.
57. Schroeder HE, Schotz AH, Wardleyrichardson T, Spencer D, Higgins TJV (1993) Transformation and regeneration of 2 cultivars of pea (Pisum sativum L.). Plant Physiol 101(3): 751-757.
58. Simonenko YV, Gleba YY, Kuchuk NV (1999) Double transformation: producing transgenic phosphinothricin-resistant plants of commercial pea lines. Russ J Plant Physiol 46(6): 804-807.
59. Sparrow PAC, Irwin JA, Dale PJ, Twyman RM, Ma JKC (2007) Pharma-planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Res 16(2): 147-161. doi: 10. 1007/s11248-007-9074-2.
60. Stoger E, Vaquero C, Torres E, Sack M, Nicholson L, Drossard J, Williams S, Keen D, Perrin Y, Christou P, Fischer R (2000) Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol Biol 42(4): 583-590.
61. Stoger E, Sack M, Perrin Y, Vaquero C, Torres E, Twyman RM, Christou P, Fischer R (2002) Practical considerations for pharmaceutical antibody production in different crop systems. Mol Breed 9(3): 149-158.
62. Stoger E, Ma JKC, Fischer R, Christou P (2005) Sowing the seeds of success: pharmaceutical proteins from plants. Curr Opin Biotech 16(2): 167-173. doi: 10. 1006/j. copbio. 2005. 01. 005.
63. Streatfield SJ (2005) Delivery of plant-derived vaccines. Expert Opin Drug Deliv 2(4): 719-728. doi: 10. 1517/17425247. 2. 4. 719.
64. Streatfield SJ, Mayor JM, Barker DK, Brooks C, Lamphear BJ, Woodard SL, Beifuss KK, Vicuna DV, Massey LA, Horn ME, Delaney DE, Nikolov ZL, Hood EE, Jilka JM, Howard JA (2002) 2000 congress symposium on molecular farming: development of an edible subunit vaccine in corn against enterotoxigenic strains of Escherichia coli. In Vitro Cell Dev Biol Plant 38(1): 11-17. doi: 10. 1079/Ivp2001247.
65. Svabova L, Griga M (2008) The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). Plant Cell Tiss Organ Cult 95(3): 293-304.
66. Svabova L, Smykal P, Griga M, Ondrej V (2005) Agrobacterium-mediated transformation of Pisum sativum in vitro and in vivo. Biol Plant49(3): 361-370.
67. Vaquero C, Sack M, Chandler J, Drossard J, Schuster F, Monecke M, Schillberg S, Fischer R (1999) Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc Natl Acad Sci USA 96(20): 11128-11133.
68. Voss A, Niersbach M, Hain R, Hirsch HJ, Liao YC, Kreuzaler F, Fischer R (1995) Reduced virus infectivity in Nicotiana-Tabacum secreting a Tmv-specific full-size antibody. Mol Breed 1(1): 39-50.
69. Walmsley AM, Arntzen CJ (2000) Plants for delivery of edible vaccines. Curr Opin Biotech 11(2): 126-129.
70. Wandelt CI, Khan MRI, Craig S, Schroeder HE, Spencer D, Higgins TJV (1992) Vicilin with carboxy-terminal Kdel is retained in the endoplasmic-reticulum and accumulates to high-levels in the leaves of transgenic plants. Plant J 2(2): 181-192.
71. Wonneberger C, Keller F (2004) Gemüsebau, Ulmer (Eugen).
72. Zhang XR, Buehner NA, Hutson AM, Estes MK, Mason HS (2006) Tomato is a highly effective vehicle for expression and oral immunization with Norwalk virus capsid protein. Plant Biotechnol J 4(4): 419-432. doi: 10. 1111/j. 1467-7652. 2006. 00191. x.
73. Zhou F, Badillo-Corona JA, Karcher D, Gonzalez-Rabade N, Piepenburg K, Borchers AMI, Maloney AP, Kavanagh TA, Gray JC, Bock R (2008) High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnol J 6(9): 897-913. doi: 10. 1111/j. 1467-7652. 2008. 00356. x.
74. Zimmermann J, Saalbach I, Jahn D, Giersberg M, Haehnel S, Wedel J, Macek J, Zoufal K, Glunder G, Falkenburg D, Kipriyanov SM (2009) Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens. BMC Biotechnol 9: 79. doi: 10. 1186/1472-6750-9-79.