Double transformation: Producing transgenic phosphinothricin-resistant plants of commercial pea lines

Russian Journal of Plant Physiology

Volumn 46, Issue 6, 1999, Pages 804-807

  Simonenko, Yu V ^a   Gleba, Yu Yu ^a   Kuchuk, N V ^a  

^a INSTITUTE OF CELL BIOLOGY AND GENETIC ENGINEERING   (Ukraine)

Author keywords

Agrobacterium tumefaciens; Bar gene; Inheritance; L phosphinothricin (BASTA); nptII gene; Pisum sativum; Transformation

Indexed keywords

EID: 0033240162     PISSN: 10214437     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Kuchuk, N.V., Geneticheskaya inzheneriya vysshikh rastenii (Genetic Engineering of Higher Plants), Kiev: Naukova Dumka, 1997.
2. Zubko, E.I., Kuchuk, N.V., Tumanova, L.G., Vikonskaya, N.A., and Gleba, Yu.Yu., Genetic Transformation of Peas Mediated by Agrobacterium tumefaciens, Biopolimery Kletka, 1990, vol. 6, pp. 77-80.
3. De Kathen, A. and Jacobsen, H.-J., Agrobacterium tumefaciens-mediated Transformation of Pisum sativum L. Using Binary and Cointegrate Vectors, Plant Cell Rep., 1990, vol. 9, pp. 276-279.
4. Puonti-Kaerlas, J., Eriksson, T., and Engstrom, P., Production of Transgenic Pea (Pisum sativum L.) Plants by Agrobacterium tumefaciens-mediated Gene Transfer, Theor. Appl. Genet., 1990, vol. 80, pp. 246-252.
5. Puonti-Kaerlas, J., Eriksson, T., and Engstrom, P., Inheritance of a Bacterial Hydromycin Phosphotransferase Gene in the Progeny of Primary Transgenic Plants, Theor. Appl. Genet., 1992, vol. 84, pp. 443-450.
6. Thompson, C.T., Movva, N., Tizard, R., Crameri, R., Davies, J., Lauwereys, M., and Botterman, J., Characterization of the Herbicide-Resistance Gene bar from Streptomyces hygroscopicus, EMBO J., 1987, vol. 6, pp. 2519-2523.
7. Shroeder, H.E., Schotz, A.H., Wardley-Richardson, T., Spencer, D., and Higgins, T.J.V., Transformation and Regeneration of Two Cultivars of Pea (Pisum sativum L.), Plant Physiol., 1993, vol. 101, pp. 751-757.
8. Bean, S., Gooding, P., Mullineaux, P., and Davies, D., A Simple System for Pea Transformation, Plant Cell Rep., 1997, vol. 16, pp. 513-519.
9. Leemans, J., Shaw, C., De Blaere, R., De Greve, H., Hernalsteens, J., Maes, M., Van Montagu, M., and Schell, J., Site-Specific Mutagenesis of Agrobacterium Ti Plasmids and Transfer of Genes to Plant Cells, J. Mol. Appl. Genet., 1981, vol. 1, pp. 149-164.
10. Birnboim, C. and Doly, J., Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA, Nucleic Acids Res., 1979, vol. 7, pp. 1513-1523.
11. Gamborg, O.L., Miller, R.A., and Ojima, K., Nutrient Requirements of Suspension Cultures of Soybean Root Cells, Exp. Cell Res., 1968, vol. 50, pp. 151-158.
12. Deak, M., Kiss, J.B., Koncz, C., and Dudits, D., Transformation of Medicago by Agrobacterium Mediated Gene Transfer, Plant Cell Rep., 1986, vol. 5, pp. 97-100.
13. Murray, M.J. and Thompson, W.E., Rapid Isolation of High Molecular Weight DNA, Nucleic Acids Res., 1980, vol. 8, pp. 4321-4325.
14. Storozhenko, S.V., Analysis of Transgenic Plants Using Polymerase Chain Reaction: A Pair of Universal Primers for Amplification of 35S-Promoter Sequence, Biopolimery Kletka, 1994, vol. 10, pp. 67-72.
15. Chuch, G.M. and Gilbert, W., Genomic Sequencing, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 1991-1995.
16. Feinberg, A.P. and Volgenstein, B., A Technique for Radiolabelling DNA Restriction Fragments to High Specific Activity, Anal. Biochem., 1984, vol. 137, pp. 266-267.