A sensitive and quantitative polymerase chain reaction-based cell free in vitro non-homologous end joining assay for hematopoietic stem cells

PLoS ONE

Volumn 7, Issue 3, 2012, Pages

  Shao, Lijian ^a   Feng, Wei ^a   Lee, Kyung Jong ^b   Chen, Benjamin P C ^b   Zhou, Daohong ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; CD38 ANTIGEN; NUCLEAR PROTEIN; PYRONINE; DNA DEPENDENT PROTEIN KINASE; HELICASE; MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL FREE SYSTEM; CELL NUCLEUS; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA STRAND BREAKAGE; GENE; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; HUMAN VERSUS ANIMAL COMPARISON; IN VITRO STUDY; LIG4 GENE; MALE; MOUSE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; ANIMAL; BIOASSAY; BONE MARROW; C57BL MOUSE; CELL CULTURE; DOUBLE STRANDED DNA BREAK; FLOW CYTOMETRY; GENETICS; METABOLISM; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL ASSAY; BLOTTING, WESTERN; BONE MARROW; CELLS, CULTURED; DNA BREAKS, DOUBLE-STRANDED; DNA END-JOINING REPAIR; DNA HELICASES; DNA-ACTIVATED PROTEIN KINASE; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELLS; HUMANS; MALE; MICE; MICE, INBRED C57BL; NUCLEAR PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 84863353872     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0033499     Document Type: Article

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