Development and evaluation of a multiplex PCR for simultaneous detection of five foodborne pathogens

Journal of Applied Microbiology

Volumn 112, Issue 4, 2012, Pages 823-830

  Chen, J ^a   Tang, J ^a   Liu, J ^a   Cai, Z ^a   Bai, X ^a  

^a SOUTHWEST UNIVERSITY FOR NATIONALITIES   (China)

Author keywords

Detection method; Enrichment; Foodborne pathogens; Multiplex PCR

Indexed keywords

COST EFFECTIVENESS; ESCHERICHIA COLI; FOOD MICROBIOLOGY; MEATS; PATHOGENS; POLYMERASE CHAIN REACTION; SALMONELLA;

CULTURE METHODS; DETECTION METHODS; ENRICHMENT; FOOD-BORNE PATHOGENS; MULTIPLEX PCR; PCR ASSAY; PCR METHOD; SIMULTANEOUS DETECTION; STAPHYLOCOCCUS AUREUS; TRADITIONAL CULTURES;

ASSAYS;

BACTERIAL DNA; DNA 16S;

BACTERIUM; BIOASSAY; DETECTION METHOD; FOOD; OPTIMIZATION; PATHOGEN; PIG; POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ESCHERICHIA COLI O157; LISTERIA MONOCYTOGENES; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PORK; RELIABILITY; SALMONELLA ENTERITIDIS; SENSITIVITY AND SPECIFICITY; SHIGELLA FLEXNERI; STAPHYLOCOCCUS AUREUS;

ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; SALMONELLA ENTERITIDIS; SHIGELLA FLEXNERI; STAPHYLOCOCCUS AUREUS;

EID: 84862811532     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2012.05240.x     Document Type: Article

References (28)

1. Abubakar, I., Irvine, L., Aldus, C.F., Wyatt, G.M., Fordham, R., Schelenz, S., Shepstone, L., Howe, A. et al. (2007) A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food. Health Technol Assess 36, 1-216.
2. Aspán, A. and Eriksson, E. (2010) Verotoxigenic Escherichia coli O157:H7 from Swedish cattle; isolates from prevalence studies versus strains linked to human infections-a retrospective study. BMC Vet Res 6, 7.
3. Critzer, F.J., Dsouza, D.H. and Golden, D.A. (2008) Transcription analysis of stx1, marA, and eaeA gene in Escherichia coli O157:H7 treated with sodium benzoate. J Food Prot 71, 1469-1474.
4. D'Souza, D.H., Critzer, F.J. and Golden, D.A. (2009) Real-time reverse-transcriptase polymerase chain reaction for the rapid detection of Salmonella using invA primers. Foodborne Pathog Dis 6, 1097-1106.
5. De Freitas, C.G., Santana, A.P., da Silva, P.H., Gonçalves, V.S., Barros Mde, A., Torres, F.A., Murata, L.S. and Perecmanis, S. (2010) PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurrence in poultry meat. Int J Food Microbiol 139, 15-22.
6. Dean-Nystrom, E.A., Melton-Celsa, A.R., Pohlenz, J.F., Moon, H.W. and O'Brien, A.D. (2003) Comparative pathogenicity of Escherichia coli O157 and intimin-negative non-O157 Shiga toxin-producing E. coli strains in neonatal pigs. Infect Immun 71, 6526-6533.
7. Deneer, H.G. and Boychuk, I. (1991) Species-specific detection of Listeria monocytogenes by DNA amplification. Appl Environ Microbiol 57, 606-609.
8. Díaz-López, A., Cantú-Ramírez, R.C., Garza-González, E., Ruiz-Tolentino, L., Tellez-Luis, S.J., Rivera, G. and Bocanegra-García, V. (2011) Prevalence of foodborne pathogens in grilled chicken from street vendors and retail outlets in reynosa, tamaulipas, Mexico. J Food Prot 74, 1320-1323.
9. Elizaquível, P. and Aznar, R. (2008) A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables. Food Microbiol 25, 705-713.
10. Fratamico, P.M. and Strobaugh, T.P. (1998) Simultaneous detection of Salmonella spp and Escherichia coli O157:H7 by multiplex PCR. J Ind Microbiol Biot 21, 92-98.
11. Gasanov, U., Hughes, D. and Hansbro, P.M. (2004) Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. FEMS Microbiol Rev 29, 851-875.
12. Hien, B.T., Scheutz, F. and Cam, P.D. (2008) Diarrheagenic Escherichia coli and Shigella Strains isolated from children in a hospital case-control study in Hanoi, Vietnam. J Clin Microbiol 46, 996-1004.
13. Jung, H.J., Park, S.H., Ha, S.D., Lee, K.H., Chung, D.H., Kim, C.H., Kim, M.G., Kim, K.Y. et al. (2009) Species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the prfA virulence gene cluster. Biosci Biotechnol Biochem 73, 1412-1415.
14. Kaper, J.B., Nataro, J.P. and Mobley, H.L. (2004) Pathogenic Escherichia coli. Nat Rev Microbiol 2, 123-140.
15. Lan, R., Stevenson, G. and Reeves, P.R. (2003) Comparison of two major forms of the Shigella virulence plasmid pINV: positive selection is a major force driving the divergence. Infect Immun 71, 6298-6306.
16. Louie, M., de Azavedo, J.C., Handelsman, M.Y., Clark C.G., Ally., B., Dytoc., M. Sherman, P. and Brunton, J. (1993) Expression and characterization of the eaeA gene product of Escherichia coli serotype O157:H7. Infect Immun 61, 4085-4092.
17. Markoulatos, P., Siafakas, N. and Moncany, M. (2002) Multiplex polymerase chain reaction: a practical approach. J Clin Lab Anal 16, 47-51.
18. Mook, P., Patel, B. and Gillespie, I.A. (2011) Risk factors for mortality in non-pregnancy-related listeriosis. Epidemiol Infect 21, 1-10.
19. Nogva, H.K., Rudi, K., Naterstad, K., Holck, A. and Lillehaug, D. (2000) Application of 5′-nuclease PCR for quantitative detection of Listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk. Appl Environ Microbiol 66, 4266-4271.
20. O'Regan, E., McCabe, E., Burgess, C., McGuinness, S., Barry, T., Duffy, G., Whyte, P. and Fanning, S. (2008) Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. BMC Microbiol 8, 156.
21. Perry, L., Heard, P., Kane, M., Kim, H., Savikhin, S., Domínguez, W. and Applegate, B. (2007) Application of multiplex polymerase chain reaction to the detection of pathogens in food. J Rapid Methods Autom Microbiol 15, 176-198.
22. Pragman, A.A. and Schliever, P.M. (2004) Virulence regulation in Staphylococcus aureus: the need for in vivo analysis of virulence factor regulation. FEMS Immunol Med Microbiol 42, 147-154.
23. Shao, Y., Zhu, S., Jin, C. and Chen, F. (2011) Development of multiplex loop-mediated isothermal amplification-RFLP (mLAMP-RFLP) to detect Salmonella spp. and Shigella spp. in milk. Int J Food Microbiol 148, 75-79.
24. Suo, B., He, Y., Tu, S.I. and Shi, X. (2010) A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products. Foodborne Pathog Dis 7, 619-628.
25. Tang, J.N., Shi, X.M., Shi, C.L. and Chen, H.C. (2006) Characterization of a duplex PCR assay for the detection of enterotoxigenic strains of Staphylococcus aureus. J Rapid Methods Autom Microbiol 14, 201-217.
26. Vu, D.T., Sethabutr, O., Von Seidlein, L., Tran, V.T., Do, G.C., Bui, T.C., Le, H.T., Lee, H. et al. (2004) Detection of Shigella by a PCR assay targeting the ipaH gene suggests increased prevalence of Shigellosis in Nha Trang, Vietnam. J Clin Microbiol 42, 2031-2035.
27. Wang, L., Li, Y. and Mustaphai, A. (2007) Rapid and simultaneous quantitation of Escherichia coli O157:H7, Salmonella, and Shigella in ground beef by multiplex real-time PCR and immunomagnetic separation. J Food Prot 70, 1366-1372.
28. Yoon, J.W. and Hovde, C.J. (2008) All blood, no stool: enterohemorrhagic Escherichia coli O157:H7 infection. J Vet Sci 9, 219-231.