Simultaneous detection of Salmonella spp and Escherichia coli 0157:H7 by multiplex PCR

Journal of Industrial Microbiology and Biotechnology

Volumn 21, Issue 3, 1998, Pages 92-98

  Fratamico, P M ^a   Strobaugh, T P ^a  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Apple cider; Bovine feces; Carcass wash water; Ground beef; Immunomagnetic separation

Indexed keywords

BACTERIAL DNA; NOVOBIOCIN; PEPTONE; PLASMID DNA; PRIMER DNA;

AGAR GEL ELECTROPHORESIS; ANIMAL TISSUE; ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL SEPARATION; CONTROLLED STUDY; ESCHERICHIA COLI; FECES; FOOD ANALYSIS; FOOD CONTAMINATION; FRUIT JUICE; MEAT; NONHUMAN; POLYMERASE CHAIN REACTION; SALMONELLA;

ANIMALIA; BACTERIA (MICROORGANISMS); BOVINAE; ESCHERICHIA COLI; ESCHERICHIA COLI 0157; MALUS X DOMESTICA; NEGIBACTERIA; SALMONELLA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0032415874     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.2900520     Document Type: Article

References (20)

1. Bean NH, JS Goulding, MT Daniels and FJ Angulo. 1997. Surveillance for foodborne disease outbreaks - United States. 1988-1992. J Food Prot 60: 1265-1286.
2. Bülte M and P Jakob. 1995. The use of a PCR-generated invA probe for the detection of Salmonella spp in artificially and naturally contaminated foods. Int J Food Microbiol 26: 335-344.
3. Burkhalter PW, C Müller, J Lüthy and U Candrian. 1995. Detection of Salmonella spp in eggs: DNA analyses, culture techniques, and serology. J AOAC Int 78: 1531-1537.
4. Buzby JC, T Roberts, C-TJ Lin and JM MacDonald. 1996. Bacterial foodborne disease: medical costs & productivity losses. Food and Consumer Economics Division, Economic Research Service, US Department of Agriculture. Agricultural Economic Report Number 741. Washington DC, USA.
5. Centers for Disease Control and Prevention. 1995. Outbreak of Salmonella serotype Typhimurium infection associated with eating raw ground beef - Wisconsin, 1994. MMWR 44: 905-909.
6. Centers for Disease Control and Prevention. 1997. Isolation of E. coli O157:H7 from sporadic cases of hemorrhagic colitis - United States. MMWR 46: 700-704.
7. Deng MY and PM Fratamico. 1996. A multiplex PCR for rapid identification of Shiga-like toxin-producing Escherichia coli O157:H7 isolated from foods. J Food Prot 59: 570-576.
8. Fratamico PM and MY Deng. 1995. Detection of Escherichia coli O157:H7 in foods by multiplex PCR. Abstract No. 128. International Association of Milk, Food, and Environmental Sanitarians, Pittsburgh, PA.
9. Galán JE and R Curtiss III. 1991. Distribution of the invA, -B, -C, and -D genes of Salmonella typhimurium among other Salmonella serovars: invA mutants of Salmonella typhi are deficient for entry into mammalian cells. Infect Immun 59: 2901-2908.
10. Gooding CM and PV Choudary. 1997. Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157:H7 in raw milk and ice-cream. J Dairy Res 64: 87-93.
11. Heuvelink AE, JTM Zwartkruis-Nahuis and E DeBoer. 1997. Evaluation of media and test kits for the detection and isolation of Escherichia coli O157 from minced beef. J Food Prot 60: 817-824.
12. Monteiro L, D Bonnemaison, A Vekris, KG Petry, J Bonnet, R Vidal, J Cabrita and F Mégraud. 1997. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol 35: 995-998.
13. Okrend AJG, BE Rose and R Matner. 1990. An improved screening method for the detection and isolation of Escherichia coli O157:H7 from meat, incorporating the 3M Petrifilm™ test kit-HEC-for hemorrhagic Escherichia coli O157:H7. J Food Prot 53: 936-940.
14. Parish ME, 1997. Public health and nonpasteurized fruit juices. Crit Rev Microbiol 23: 109-119.
15. Pegues DA, EL Hohmann and SI Miller. 1995. Infections of the gastrointestinal tract. In: Salmonella including S. typhi (Blaser MJ, PD Smith, JI Ravdin, HB Greenberg and RL Guerrant, eds), pp 785-809, Raven Press, New York.
16. Rossen L, P Nørskov, K Holmstrøm and OF Rasmussen. 1992. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol 17: 37-45.
17. Schmidt H, H Karch and L Beutin. 1994. The large-sized plasmids of enterohemorrhagic Escherichia coli O157 strains encode hemolysins which are presumably members of the E. coli α-hemolysin family. FEMS Microbiol Lett 117: 189-196.
18. Tilden J, W Young, AM McNamara, C Custer, B Boesel, MA Lambert-Fair, J Majkowski, D Bugia, SB Werner, J Hollingsworth and JG Morris. 1996. A new route of transmission for Escherichia coli: infection from dry fermented salami. Am J Public Health 86: 1142-1145.
19. Wachsmuth IK, PH Sparling, TJ Barrett and ME Potter. 1997. Enterohemorrhagic Escherichia coli in the United States. FEMS Immunol Med Microbiol 18: 233-239.
20. Yu H and JG Bruno. 1996. Immunomagnetic-electrochemiluminescent detection of Escherichia coli O157 and Salmonella typhimurium in foods and environmental water samples. Appl Environ Microbiol 62: 587-592.