Characterization of a duplex polymerase chain reaction assay for the detection of enterotoxigenic strains of Staphylococcus aureus

Journal of Rapid Methods and Automation in Microbiology

Volumn 14, Issue 3, 2006, Pages 201-217

  Tang, Jun Ni ^a,b   Shi, Xian Ming ^a   Shi, Chun Lei ^a   Chen, Huan Chun ^b  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

STAPHYLOCOCCUS AUREUS;

EID: 33749442297     PISSN: 10603999     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-4581.2006.00047.x     Document Type: Article

References (37)

1. ALTSCHUL, S.F., MADDEN, T.L., SCHAFFER, A.A., ZHANG, J., ZHANG, Z., MILLER, W. and LIPMAN, D.J. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402.
2. BALABAN, N. and RASOOLY, A. 2000. Staphylococcal enterotoxins. Int. J. Food Microbiol. 61, 1-10.
3. BRAKSTAD, O.G., AASBAKK, K. and MAELAND, J.A. 1992. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbiol. 30, 1654-1660.
4. CHESNEAU, O., ALLIGNET, J. and SOLH, E.L.N. 1993. Thermonuclease gene as a target nucleotide sequence for specific recognition of Staphylococcus aureus. Mol. Cell. Probes 7, 301-310.
5. CREMONESI, P., LUZZANA, M., BRASCA, M., MORANDI, S., LODI, R., VIMERCATI, C., AGNELLINI, D., CARAMENTI, G., MORONI, P. and CASTIGLIONI, B. 2005. Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Mol. Cell. Probes 19, 299-305.
6. DINGES, M.M., ORWIN, P.M. and SCHLIEVERT, P.M. 2000. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 13, 16-34.
7. HEIN, I., LEHNER, A., RIECK, P., KLEIN, K., BRANDL, E. and WAGNER, M. 2001. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Appl. Environ. Microbiol. 67, 3122-3126.
8. IANDOLO, J.J. 1989. Genetic analysis of extracellular toxins of Staphylococcus aureus. Annu. Rev. Microbiol. 43, 375-402.
9. INGRAHAM, J.L. and INGRAHAM, C.A. 2000. Introduction to Microbiology: A case-history study approach, 2nd Ed., Brooks/Cole Publishing Co., Pacific Grove, CA.
10. JORGENSEN, H.J., MORK, T., HOGASEN, H.R. and RORVIK, L.M. 2005. Enterotoxigenic Staphylococcus aureus in bulk milk in Norway. J. Appl. Microbiol. 99, 158-166.
11. KALIA, A., RATTAN, A. and CHOPRA, P. 1999. A method for extraction of high-quality and high-quantity genomic DNA generally applicable to pathogenic bacteria. Anal. Biochem. 275, 1-5.
12. KIM, C.H., KHAN, M., MORIN, D.E., HURLEY, W.L., TRIPATHY, D.N., KEHRLI, M., OLUOCH, J.R.A.O., KAKOMA. I. 2001. Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk. J. Dairy Sci. 84, 74-83.
13. KUMAR, J.K., SHARMA, A.K. and KULKARNI, P.R. 2000a. Effect of preservation techniques and food additives on staphylococcal thermonuclease. Nahrung 44, 272-275.
14. KUMAR, J.K., SHARMA, A.K. and KULKARNI, P.R. 2000b. Effect of gamma irradiation on staphylococcal thermonuclease (TNase). Int. J. Food Sci. Nutr. 51, 55-58.
15. KURODA, M., OHTA, T., UCHIYAMA, I., BABA, T., YUZAWA, H., KOBAYASHI, I., CUI, L., OGUCHI, A., AOKI, K., NAGAI, Y., ET AL. 2001. Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357, 1225-1240.
16. LACHICA, R.V.F., WEISS, K.F. and DEIBEL, R.H. 1969. Relationships among coagulase, enterotoxin, and heat stable deoxyribonuclease production by Staphylococcus aureus. Appl. Microbiol. 18, 126-127.
17. LACHICA, R.V.F., GENIGEORGIS, C. and HOEPRICH, P.D. 1971. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 21, 585-587.
18. LETERTRE, C., PERELLE, S., DILASSER, F. and FACH, P. 2003. Detection and genotyping by real-time PCR of the staphylococcal enterotoxin genes sea to sej. Mol. Cell. Probes 17, 139-147.
19. MALORNY, B., TASSIOS, P.T., RADSTROM, P., COOK, N., WAGNER, M. and HOORFAR, J. 2003. Standardization of diagnostic PCR for the detection of food-borne pathogens. Int. J. Food Microbiol. 83, 39-48.
20. MARRACK, P. and KAPPLER, J. 1990. The staphylococcal enterotoxins and their relatives. Science 248, 705-711.
21. MARTIN, M.C., GONZALEZ-HEVIA, M.A. and MENDOZA, M.C. 2003. Usefulness of a two-step PCR procedure for detection and identification of enterotoxigenic staphylococci of bacterial isolates and food samples. Food Microbiol. 20, 605-610.
22. MEHROTRA, M., WANG, G. and JOHNSON, W.M. 2000. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 38, 1032-1035.
23. MEYRAND, A., BOUTRAND-LOEI, S., RAY-GUENIOT, S., MAZUY, C., GASPARD, C.E., JAUBERT, G., PERRIN, G., LAPEYRE, C. and VERNOZY-ROZAND, C. 1998. Growth and enterotoxin production of Staphylococcus aureus during the manufacture and ripening of Camembert type cheeses from raw goat's milk. J. Appl. Microbiol. 85, 537-544.
24. MEYRAND, A., ATRACHE, V., BAVAI, C., MONTET, M.P. and VERNOZY-ROZAND, C. 1999. An automated method for the detection of staphylococcal heat stable deoxyribonuclease in dairy products. Lett. Appl. Microbiol. 29, 216-220.
25. MLYNARCZYK, A., MLYNARCZYK, G., SZYMANOWSKA, A., STANCZAK, J., LUCZAK, M. and JELJASZEWICZ, J. 2000. Use of PCR for evaluating detection of coa and nuc genes in methicillin-resistant, coagulase-negative strains of Staphylococcus aureus (MRSA-CN). Med. Dosw. Mikrobiol. 52, 217-222.
26. OMOE, K., ISHIKAWA, M., SHIMODA, Y., HU, D.L., UEDA, S. and SHINAGAWA, K. 2002. Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes. J. Clin. Microbiol. 40, 857-862.
27. PALOMARES, C., TORRES, M.J., TORRES, A., AZNAR, J. and PALOMARES, J.C. 2003. Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR. Diagn. Microbiol. Infect. Dis. 45, 183-189.
28. RAMESH, A., PADMAPRIYA, B.P., CHRASHEKAR, A. and VARADARAJ, M.C. 2002. Application of a convenient DNA extraction method and multiplex PCR for the direct detection of Staphylococcus aureus and Yersinia enterocolitica in milk samples. Mol. Cell. Probes 16, 307-314.
29. REN, K., BANNAN, J.D., PANCHOLI, V., CHEUNG, A.L., ROBBINS, J.C., FISCHETTI, V.A. and ZABRISKIE, J.B. 1994. Characterization and biological properties of a new staphylococcal exotoxin. J. Exp. Med. 180, 1675-1683.
30. ROLFS, A., SCHULLER, I., FINCKH, U. and WEBER-ROLFS, I. 1992. PCR principles and reaction components. In PCR: Clinical Diagnostics and Research (A. Rolfs, I. Schuller, U. Finckh and I. Weber-Rolfs, eds.) pp. 1-18, Springer, Berlin, Germany.
31. SALISBURY, S.M., SABATINI, L.M. and SPIEGEL, C.A. 1996. Identification of methicillin-resistant staphylococci by multiplex polymerase chain reaction assay. Am. J. Clin. Pathol. 107, 368-373.
32. SU, Y.C. and WONG, A.C. 1995. Identification and purification of a new staphylococcal enterotoxin, H. Appl. Environ. Microbiol. 61, 1438-1443.
33. TAMARAPU, S., MCKILLIP, J.L. and DRAKE, M.A. 2001. Development of a multiplex polymerase chain reaction assay for detection and differentiation of Staphylococcus aureus in diary products. J. Food Prot. 64, 664-668.
34. THOMSON, J.D., HIGGINS, D.G. and GIBSON, T.J. 1994. CLUSTAL W: Improving the sensitivity of progressive sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
35. VERNOZY-ROZAND, C., MEYRAND, A., MAZUY, C., DELIGNETTE-MULLER, M.L., JAUBERT, G., PERRIN, G., LAPEYRE, C. and RICHARD, Y. 1998. Behavior and enterotoxin production of Staphylococcus aureus during the manufacture and ripening of raw goat's milk lactic cheeses. J. Dairy Res. 65, 273-281.
36. WANG, S.J., CHOW, L.W. and WU, M.J. 2002. Multiplex PCR for the simultaneous detection of the SEA, SEB, SEC, SED and SEE genes of enterotoxigenic Staphylococcus aureus. J. Food Drug Anal. 10, 164-169.
37. WILSON, I.G., COOPER, J.E. and GILMOUR, A. 1991. Detection of enterotoxigenic Staphylococcus aureus in dried skimmed milk: Use of the polymerase chain reaction for amplification and detection of staphylococcal enterotoxin genes entB and entC1 and the thermonuclease gene nuc. Appl. Environ. Microbiol. 57, 1793-1798.