Detection of Shigella by a PCR Assay Targeting the ipaH Gene Suggests Increased Prevalence of Shigellosis in Nha Trang, Vietnam

Journal of Clinical Microbiology

Volumn 42, Issue 5, 2004, Pages 2031-2035

  Thiem, Vu Dinh ^a   Sethabutr, Orntipa ^d   Von Seidlein, Lorenz ^e   Van Tung, Tran ^b   Canh, Do Gia ^a   Chien, Bui Trong ^b   Tho, Le Huu ^c   Lee, Hyejon ^e   Houng, Huo Shu ^f   Hale, Thomas L ^f   Clemens, John D ^e   Mason, Carl ^d   Trach, Dang Duc ^a  

^a National Institute of Hygiene and Epidemiology   (Viet Nam)

^b NHA TRANG PASTEUR INSTITUTE   (Viet Nam)

^c Health Service Office of Khanh Hoa   (Viet Nam)

^d ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES   (Thailand)

^e International Vaccine Institute   (South Korea)

^f WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CLINICAL FEATURE; CONTROLLED STUDY; DIARRHEA; DNA DETERMINATION; DYSENTERY; GENE IDENTIFICATION; GENE SEQUENCE; GENE TARGETING; GRAM NEGATIVE BACTERIUM; HUMAN; IPAH GENE; MAJOR CLINICAL STUDY; NUCLEOTIDE SEQUENCE; PATIENT CARE; POLYMERASE CHAIN REACTION; PREVALENCE; PRIORITY JOURNAL; RECTUM; SHIGELLA; SHIGELLOSIS; SPECIES DIFFERENCE; VIET NAM;

ADOLESCENT; ADULT; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BASE SEQUENCE; CHILD; CHILD, PRESCHOOL; DNA, BACTERIAL; DYSENTERY, BACILLARY; FEMALE; GENES, VIRAL; HUMANS; INFANT; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; SHIGELLA; VIETNAM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SHIGELLA;

EID: 2442568863     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.5.2031-2035.2004     Document Type: Article

References (19)

1. Dutta, S., A. Chatterjee, P. Dutta, K. Rajendran, S. Roy, K. C. Pramanik, and S. K. Bhattacharya. 2001. Sensitivity and performance characteristics of a direct PCR with stool samples in comparison to conventional techniques for diagnosis of Shigella and enteroinvasive Escherichia coli infection in children with acute diarrhoea in Calcutta, India. J. Med. Microbiol. 50:667-674.
2. Echeverria, P., O. Sethabutr, and C. Pitarangsi. 1991. Microbiology and diagnosis of infections with Shigella and enteroinvasive Escherichia coli. Rev. Infect. Dis. 13(Suppl. 4):S220-S225.
3. Frankel, G., L. Riley, J. A. Giron, J. Valmassoi, A. Friedmann, N. Strockbine, S. Falkow, and G. K. Schoolnik. 1990. Detection of Shigella in feces using DNA amplification. J. Infect. Dis. 161:1252-1256.
4. Gaudio, P. A., O. Sethabutr, P. Echeverria, and C. W. Hoge. 1997. Utility of a polymerase chain reaction diagnostic system in a study of the epidemiology of shigellosis among dysentery patients, family contacts, and well controls living in a shigellosis-endemic area. J. Infect. Dis. 176:1013-1018.
5. Hartman, A. B., M. Venkatesan, E. V. Oaks, and J. M. Buysse. 1990. Sequence and molecular characterization of a multicopy invasion plasmid antigen gene, ipaH, of Shigella flexneri. J. Bacteriol. 172:1905-1915.
6. Houng, H. S., O. Sethabutr, and P. Echeverria. 1997. A simple polymerase chain reaction technique to detect and differentiate Shigella and enteroinvasive Escherichia coli in human feces. Diagn. Microbiol. Infect. Dis. 28:19-25.
7. Islam, D., and A. A. Lindberg. 1992. Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction. J. Clin. Microbiol. 30:2801-2806.
8. Islam, M. S., M. S. Hossain, M. K. Hasan, M. M. Rahman, G. Fuchs, D. Mahalanabis, A. H. Baqui, and M. J. Albert. 1998. Detection of shigellae from stools of dysentery patients by culture and polymerase chain reaction techniques. J. Diarrhoeal Dis. Res. 16:248-251.
9. Kotloff, K. L., J. P. Winickoff, B. Ivanoff, J. D. Clemens, D. L. Swerdlow, P. J. Sansonetti, G. K. Adak, and M. M. Levine. 1999. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull. W. H. O. 77:651-666.
10. Lampel, K. A., J. A. Jagow, M. Trucksess, and W. E. Hill. 1990. Polymerase chain reaction for detection of invasive Shigella flexneri in food. Appl. Environ. Microbiol. 56:1536-1540.
11. Lindqvist, R. 1999. Detection of Shigella spp. in food with a nested PCR method-sensitivity and performance compared with a conventional culture method. J. Appl. Microbiol. 86:971-978.
12. Oyofo, B. A., Z. S. Mohran, S. H. el-Etr, M. O. Wasfy, and L. F. Peruski, Jr. 1996. Detection of enterotoxigenic Escherichia coli, Shigella and Campylobacter spp. by multiplex PCR assay. J. Diarrhoeal Dis. Res. 14:207-210.
13. Phantouamath, B., N. Sithivong, S. Insisiengmay, N. Higa, C. Toma, N. Nakasone, and M. Iwanaga. 2003. The incidence of Escherichia coli having pathogenic genes for diarrhea: a study in the People's Democratic Republic of Lao. Jpn. J. Infect. Dis. 56:103-106.
14. Sansonetti, P. J., G. Tran Van Nhieu, and C. Egile. 1999. Rupture of the intestinal epithelial barrier and mucosal invasion by Shigella flexneri. Clin. Infect. Dis. 28:466-475.
15. Sethabutr, O., P. Echeverria, C. W. Hoge, L. Bodhidatta, and C. Pitarangsi. 1994. Detection of Shigella and enteroinvasive Escherichia coli by PCR in the stools of patients with dysentery in Thailand. J. Diarrhoeal Dis. Res. 12:265-269.
16. Sethabutr, O., M. Venkatesan, G. S. Murphy, B. Eampokalap, C. W. Hoge, and P. Echeverria. 1993. Detection of shigellae and enteroinvasive Escherichia coli by amplification of the invasion plasmid antigen H DNA sequence in patients with dysentery. J. Infect. Dis. 167:458-461.
17. Taylor, W. I., and D. Schelhart. 1975. Effect of temperature on transport and plating media for enteric pathogens. J. Clin. Microbiol. 2:281-286.
18. Villalobo, E., and A. Torres. 1998. PCR for detection of Shigella spp. in mayonnaise. Appl. Environ. Microbiol. 64:1242-1245.
19. Ye, L. Y., F. H. Lan, Z. Y. Zhu, X. M. Chen, and X. L. Ye. 1993. Detection of Shigella and enteroinvasive Escherichia coli using polymerase chain reaction. J. Diarrhoeal Dis. Res. 11:38-40.