Mini-scale bioprocessing systems for highly parallel animal cell cultures

Biotechnology Progress

Volumn 28, Issue 3, 2012, Pages 595-607

  Kim, Beum Jun ^a   Diao, Jinpian ^b   Shuler, Michael L ^a  

^a Cornell University   (United States)

^b GENENTECH INC   (United States)

Author keywords

Animal cell culture; Automation; Miniaturized bioreactor; Monitoring controlling devices; Performance evaluation; Process development

Indexed keywords

ACTIVE MIXING; ANIMAL CELLS; BIOPROCESSING; CELL DENSITY; CELL VIABILITY; CURRENT STATUS; DISSOLVED CARBON DIOXIDE; ENGINEERING PARAMETERS; FED BATCHES; HIGH-THROUGHPUT; MICROFLUIDIC CELL CULTURE; MICROTITER PLATES; NOVEL DESIGN; ON-LINE MEASUREMENT; PARALLEL OPERATIONS; PERFORMANCE EVALUATION; PROCESS DEVELOPMENT; PROCESS PERFORMANCE; STIRRED TANK REACTORS; THERAPEUTIC PROTEIN;

AUTOMATION; BIOCONVERSION; BIOREACTORS; CARBON DIOXIDE; PROTEINS;

ANIMAL CELL CULTURE;

ANIMAL; ARTICLE; BIOREACTOR; CULTURE TECHNIQUE; ELECTRONICS; EQUIPMENT DESIGN;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; EQUIPMENT DESIGN; MINIATURIZATION;

ANIMALIA;

EID: 84862184762     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1554     Document Type: Article

References (78)

1. Doig SD, Diep A, Baganz F. Characterisation of a novel miniaturised bubble column bioreactor for high throughput cell cultivation. Biochem Eng J. 2005; 23: 97-105.
2. Weuster-Botz D. Parallel reactor systems for bioprocess development. Technology Transfer in Biotechnology: From Lab to Industry to Production. Berlin: Springer-Verlag Berlin, 2005: 125-143.
3. Chen A, Chitta R, Chang D, Anianullah A. Twenty-four well plate miniature bioreactor system as a scale-down model for cell culture process development. Biotechnol Bioeng. 2009; 102: 148-160.
4. MacBeath G, Schreiber SL. Printing proteins as microarrays for high-throughput function determination. Science. 2000; 289: 1760-1763.
5. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen ZT, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer MLI, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu PG, Begley RF, Rothberg JM. Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005; 437: 376-380.
6. Schena M, Shalon D, Davis RW, Brown PO. Quantitative monitoring of gene-expression patterns with a complementary-DNA microarray. Science. 1995; 270: 467-470.
7. Pörtner R. Characteristics of mammalian cells and requirements for cultivation. In: Eibl R, Eibl D, Pörtner R, Catapano G, Czermak P, editors. Cell and Tissue Reaction Engineering. Berlin Heidelberg: Springer, 2009: 13-53.
8. Vallejos JR, Moreira A, Rao G. Integrating process scouting devices (psds) with bench-scale devices: Challenges and opportunites for mammalian cell culture. In: Moo-Young M, editor. Comprehensive Biotechnology, 2nd ed. Waltham, MA: Elsevier, 2011: 659-667.
9. Bareither R, Pollard D. A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnol Prog. 2011; 27: 2-14.
10. Hu WS, Aunins JG. Large-scale mammalian cell culture. Curr Opin Biotechnol. 1997; 8: 148-153.
11. Betts JI, Baganz F. Miniature bioreactors: current practices and future opportunities. Microb Cell Fact. 2006; 5: 21.
12. Kumar S, Wittmann C, Heinzle E. Minibioreactors. Biotechnol Lett. 2004; 26: 1-10.
13. Girard P, Jordan M, Tsao M, Wurm FM. Small-scale bioreactor system for process development and optimization. Biochem Eng J. 2001; 7: 117-119.
14. Isett K, George H, Herber W, Amanullah A. Twenty-four-well plate miniature bioreactor high-throughput system: assessment for microbial cultivations. Biotechnol Bioeng. 2007; 98: 1017-1028.
15. Barrett TA, Wu A, Zhang H, Levy MS, Lye GJ. Microwell engineering characterization for mammalian cell culture process development. Biotechnol Bioeng. 2010; 105: 260-275.
16. Mere L, Bennett T, Coassin P, England P, Hamman B, Rink T, Zimmerman S, Negulescu P. Miniaturized fret assays and microfluidics: key components for ultra-high-throughput screening. Drug Discov Today. 1999; 4: 363-369.
17. De Jesus MJ, Girard P, Bourgeois M, Baumgartner G, Jacko B, Amstutz H, Wurm FM. Tubespin satellites: a fast track approach for process development with animal cells using shaking technology. Biochem Eng J. 2004; 17: 217-223.
18. Kondragunta B, Drew JL, Brorson KA, Moreira AR, Rao G. Advances in clone selection using high-throughput bioreactors. Biotechnol Prog. 2010; 26: 1095-1103.
19. Ge XD, Hanson M, Shen H, Kostov Y, Brorson KA, Frey DD, Moreira AR, Rao G. Validation of an optical sensor-based high-throughput bioreactor system for mammalian cell culture. J Biotechnol. 2006; 122: 293-306.
20. Diao JP, Young L, Zhou P, Shuler ML. An actively mixed mini-bioreactor for protein production from suspended animal cells. Biotechnol Bioeng. 2008; 100: 72-81.
21. Kim BJ, Zhao T, Young L, Zhou P, Shuler ML. Batch, fed-batch, and microcarrier cultures with cho cell lines in a pressure-cycle driven miniaturized bioreactor. Biotechnol Bioeng. 2012; 109: 137-145.
22. Legmann R, Schreyer HB, Combs RG, McCormick EL, Russo AP, Rodgers ST. A predictive high-throughput scale-down model of monoclonal antibody production in cho cells. Biotechnol Bioeng. 2009; 104: 1107-1120.
23. Amanullah A, Otero JM, Mikola M, Hsu A, Zhang JY, Aunins J, Schreyer HB, Hope JA, Russo AP. Novel micro-bioreactor high throughput technology for cell culture process development: reproducibility and scalability assessment of fed-batch cho cultures. Biotechnol Bioeng. 2010; 106: 57-67.
24. Silk NJ, Denby S, Lewis G, Kuiper M, Hatton D, Field R, Baganz F, Lye GJ. Fed-batch operation of an industrial cell culture process in shaken microwells. Biotechnol Lett. 2010; 32: 73-78.
25. Schmitmeier S, Langsch A, Jasmund I, Bader A. Development and characterization of a small-scale bioreactor based on a bioartificial hepatic culture model for predictive pharmacological in vitro screenings. Biotechnol Bioeng. 2006; 95: 1198-1206.
26. Bartholomew SR, Tansey JT. Cost-effective engineering of a small-scale bioreactor. Biotechnol Bioeng. 2007; 96: 401-407.
27. 2 incubator. Cytotechnology. 1994; 14: 1-9.
28. Hufnagel H, Huebner A, Gulch C, Guse K, Abell C, Hollfelder F. An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab Chip. 2009; 9: 1576-1582.
29. Moore SK, Kleis SJ. Characterization of a novel miniature cell culture device. Acta Astronaut. 2008; 62: 632-638.
30. Prokop A, Prokop Z, Schaffer D, Kozlov E, Wikswo J, Cliffel D, Baudenbacher F. Nanoliterbioreactor: long-term mammalian cell culture at nanofabricated scale. Biomed Microdevices. 2004; 6: 325-339.
31. Charnley M, Textor M, Khademhosseini A, Lutolf MP. Integration column: microwell arrays for mammalian cell culture. Integr Biol. 2009; 1: 625-634.
32. Nevill JT, Cooper R, Dueck M, Breslauer DN, Lee LP. Integrated microfluidic cell culture and lysis on a chip. Lab Chip. 2007; 7: 1689-1695.
33. Zhang B, Kim M-C, Thorsen T, Wang Z. A self-contained microfluidic cell culture system. Biomed Microdevices. 2009; 11: 1233-1237.
34. Yang ST, Zhang XD, Wen Y. Microbioreactors for high-throughput cytotoxicity assays. Curr Opin Drug Discov Devel. 2008; 11: 111-127.
35. Flickinger MC, Drew SW. The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation. New York: Wiley, 1999.
36. 2 and pH sensors using microelectronic techniques. Diabetes Care. 1982; 5: 275-277.
37. Maharbiz MM, Holtz WJ, Howe RT, Keasling JD. Microbioreactor arrays with parametric control for high-throughput experimentation. Biotechnol Bioeng. 2004; 85: 376-381.
38. Kermis HR, Kostov Y, Rao G. Rapid method for the preparation of a robust optical pH sensor. Analyst. 2003; 128: 1181-1186.
39. Kostov Y, Harms P, Randers-Eichhorn L, Rao G. Low-cost microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng. 2001; 72: 346-352.
40. Szita N, Boccazzi P, Zhang ZY, Boyle P, Sinskey AJ, Jensen KF. Development of a multiplexed microbioreactor system for high-throughput bioprocessing. Lab Chip. 2005; 5: 819-826.
41. Zanzotto A, Szita N, Boccazzi P, Lessard P, Sinskey AJ, Jensen KF. Membrane-aerated microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng. 2004; 87: 243-254.
42. Zhang ZY, Szita N, Boccazzi P, Sinskey AJ, Jensen KF. A well-mixed, polymer-based microbioreactor with integrated optical measurements. Biotechnol Bioeng. 2006; 93: 286-296.
43. Lamping SR, Zhang H, Allen B, Shamlou PA. Design of a prototype miniature bioreactor for high throughput automated bioprocessing. Chem Eng Sci. 2003; 58: 747-758.
44. Harms P, Kostov Y, Rao G. Bioprocess monitoring. Curr Opin Biotechnol. 2002; 13: 124-127.
45. Koncki R, Wolfbeis OS. Composite films of prussian blue and n-substituted polypyrroles: Fabrication and application to optical determination of pH. Anal Chem. 1998; 70: 2544-2550.
46. Sotomayor PT, Raimundo IM, Zarbin AJG, Rohwedder JJR, Neto GO, Alves OL. Construction and evaluation of an optical pH sensor based on polyaniline-porous vycor glass nanocomposite. Sensor Actuator B: Chem. 2001; 74: 157-162.
47. Krommenhoek EE, van Lceuwen M, Gardeniers H, van Gulik WM, van den Berg A, Li XN, Ottens M, van der Wielen LAM, Heiinen JJ. Lab-scale fermentation tests of microchip with integrated electrochemical sensors for pH, temperature, dissolved oxygen and viable biomass concentration. Biotechnol Bioeng. 2008; 99: 884-892.
48. Schapper D, Alam M, Szita N, Lantz AE, Gernaey KV. Application of microbioreactors in fermentation process development: a review. Anal Bioanal Chem. 2009; 395: 679-695.
49. Wu MH, Lin JL, Wang JB, Cui ZF, Cui Z. Development of high throughput optical sensor array for on-line pH monitoring in micro-scale cell culture environment. Biomed Microdevices. 2009; 11: 265-273.
50. Johnson MJ, Borkowski J, Engblom C. Steam sterilizable probes for dissolved oxygen measurement. Biotechnol Bioeng. 2000; 67: 645-656; reprinted from biotechnology and bioengineering, vol 6, pg 457, 1964.
51. Bambot SB, Holavanahali R, Lakowicz JR, Carter GM, Rao G. Phase fluorometric sterilizable optical oxygen sensor. Biotechnol Bioeng. 1994; 43: 1139-1145.
52. Lee YH, Tsao M. Dissolved oxygen electrodes. In: Ghose TK, Fiechter A, Blackebrough N, editors. Advances in Biochemical Engineering/Biotechnology. Berlin/Heidelberg: Springer-Verlag, 1979: 35-86.
53. 2 during fermentations. J Microbiol Meth. 1989; 10: 155-176.
54. 2 ventilation on microbial cultivation in shake-flasks. Biotechnol Tech. 1998; 12: 325-328.
55. 2 methods. Biotechnol Bioeng. 2003; 83: 312-320.
56. 2 concentration on growth of rhizopus oligosporus nrrl 2710. J Appl Microbiol. 2002; 92: 329-337.
57. 2 electrode. Biotechnol Bioeng. 1980; 22: 2411-2416.
58. 2 determination. J Appl Physiol. 1958; 13: 515-520.
59. Shoda M, Ishikawa Y. Carbon-dioxide sensor for fermentation systems. Biotechnol Bioeng. 1981; 23: 461-466.
60. Pattison RN, Swamy J, Mendenhall B, Hwang C, Frohlich BT. Measurement and control of dissolved carbon dioxide in mammalian cell culture processes using an in situ fiber optic chemical sensor. Biotechnol Prog. 2000; 16: 769-774.
61. Uttamlal M, Walt DR. A fiberoptic carbon-dioxide sensor for fermentation monitoring. Biotechnology. 1995; 13: 597-601.
62. 2 sensor. Biosens Bioelectron. 2003; 18: 857-865.
63. 2 sensing system for fermentation and cell culture. Biotechnol Bioeng. 2005; 89: 329-334.
64. Lee HLT, Boccazzi P, Ram RJ, Sinskey AJ. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control. Lab Chip. 2006; 6: 1229-1235.
65. Joeris K, Frerichs JG, Konstantinov K, Scheper T. In-situ microscopy: online process monitoring of mammalian cell cultures. Cytotechnology. 2002; 38: 129-134.
66. Walther I, van der Schoot B, Boillat M, Muller O, Cogoli A. Microtechnology in space bioreactors. Chimia. 1999; 53: 75-80.
67. Kim JW, Lee YH. Development of microfermenter chip. J Korean Phys Soc. 1998; 33: S462-S466.
68. Janelt G, Gerbsch N, Buchholz R. A novel fiber optic probe for on-line monitoring of biomass concentrations. Bioprocess Eng. 2000; 22: 275-279.
69. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F. Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis). Cytometry. 1997; 27: 1-20.
70. Buhlmann C, Preckel T, Chan S, Luedke G, Valer M. A new tool for routine testing of cellular protein expression: integration of cell staining and analysis of protein expression on a microfluidic chip-based system. J Biomol Tech. 2003; 14: 119-127.
71. Goergen JL, Marc A, Engasser JM. Determination of cell-lysis and death kinetics in continuous hybridoma cultures from the measurement of lactate-dehydrogenase release. Cytotechnology. 1993; 11: 189-195.
72. Wodnicka M, Guarino RD, Hemperly JJ, Timmins MR, Stitt D, Pitner JB. Novel fluorescent technology platform for high throughput cytotoxicity and proliferation assays. J Biomol Screen. 2000; 5: 141-152.
73. O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem. 2000; 267: 5421-5426.
74. Carvell JP, Dowd JE. On-line measurements and control of viable cell density in cell culture manufacturing processes using radio-frequency impedance. Cytotechnology. 2006; 50: 35-48.
75. Van der Weide DW, Blattner FR. Microfabricated microbial growth assay method and apparatus. US 6649402, November 18, 2003.
76. Yang Y, Chae J. Miniaturized protein separation using a liquid chromatography column on a flexible substrate. J Micromech Microeng. 2008; 18: 125010.
77. Diao J. Development of Microfluidic Devices to Probe Chemotaxis and Therapeutic Protein Production. Ithaca: Cornell University, 2007.
78. Karlberg B, Pacey GE. Flow injection analysis: a practical guide. Amsterdam, New York: Elsevier, 1989.