An actively mixed mini-bioreactor for protein production from suspended animal cells

Biotechnology and Bioengineering

Volumn 100, Issue 1, 2008, Pages 72-81

  Diao, Jinpian ^a   Young, Lincoln ^b   Zhou, Peng ^b   Shuler, Michael Louis ^c  

^a GENENTECH INC   (United States)

^b Kionix Inc   (United States)

^c School of Operations Research and Industrial Engineering   (United States)

Author keywords

Animal cell culture; Microbioreactor; Protein production

Indexed keywords

BIOPHARMACEUTICAL PRODUCTION; MICROBIOREACTORS; PROTEIN PRODUCTION;

ANIMAL CELL CULTURE; OPTIMIZATION; PARAMETER ESTIMATION; PROTEINS;

BIOREACTORS;

ALKALINE PHOSPHATASE; ALKENE DERIVATIVE; CARBON DIOXIDE; OXYGEN; POLYMETHYLPENTENE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL DENSITY; CELL GROWTH; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; INSECT CELL; INSECT CELL CULTURE; LEPIDOPTERA; MEDICAL RESEARCH; NONHUMAN; PHASE TRANSITION; PROTEIN SYNTHESIS; SHEAR STRESS;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; MICROFLUIDICS; RECOMBINANT PROTEINS; SPODOPTERA;

ANIMALIA; HEXAPODA; SPODOPTERA FRUGIPERDA;

EID: 42549126345     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21751     Document Type: Article

References (42)

1. Bhatia R, Jesionowski G, Ferrance I, Ataai MM. 1997. Insect cell physiology. Cytotechnology 24:1-9.
2. Brandrup J, Immergut EH, editors. 1975. Polymer handbook. New York: lohn Wiley & Sons.
3. Chu L, Robinson DK. 2001. Industrial choices for protein production by large-scale cell culture. Curr Opin Biotechnol 12(2):180-187.
4. Cowger NJ, O'Connor KC, Hammond TG, Lacks DJ, Navar GL. 1999. Characterization of bimodal cell death of insect cells in a rotating-wall vessel and shaker flask. Biotechnol Bioeng 64:14-26.
5. Cussler EL. 1997. Diffusion: Mass transfer in fluid system. Cambridge: Cambridge university press.
6. Dee KU, Shuler ML. 1997. Optimization of an assay for baculovirus titer and design of regimens for the synchronous infection of insect cells. Biotechnol Prog 13(1):14-24.
7. Flickinger MC, Drew SW, editors. 1999. Encyclopedia of bioprocess technology - Fermentation, biocatalysis, and bioseparation. New York: John Wiley & Sons.
8. Garnier A, Voyer R, Tom R, Perret S, Jardin B, Kamen A. 1996. Dissolved carbon dioxide accumulation in a large scale and high density production of TGFβ with baculovirus infected Sf-9 cells. Cytotechnology 22: 53-63.
9. 2 sensor. Biosens Bioelectron 18(7):857-865.
10. 2 sensing system for fermentation and cell culture. Biotechnol Bioeng 89(3):329-334.
11. Henzel WJ, Billed TM, Stults JT, Wong SC, Grimley C, Watanabe C. 1993. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Natl Acad Sci 90(11):5011-5015.
12. Humphery-Smith I, Cordwell SJ, Blackstock WP. 1997. Proteome research: Complementarity and limitations with respect to the RNA and DNA worlds. Electrophoresis 18(8):1217-1242.
13. Kamen AA, Thom RL, Caron AW, Chavarie C, Massie B, Archambault J. 1991. Culture of insect cells in a helical ribbon impeller bioreactor. Biotechnol Bioeng 38:619-628.
14. Klose J, Kobalz U. 1995. Two-dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome. Electrophoresis 16(6):1034-1059.
15. Kostov Y, Harms P, Randers-Eichhorn L, Rao G. 2001. Low-cost microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng 72(3):346-352.
16. Kumar S, Wittmann C, Heinzle E. 2004. Minibioreactors. Biotechnol Lett 26(1):1-10.
17. Lamping SR, Zhang H, Allen B, Ayazi Shamlou P. 2003. Design of a prototype miniature bioreactor for high-throughput automated bioprocessing. Chem Eng Sci 58(3):747-758.
18. Lee HLT, Boccazzi P, Ram RJ, Sinskey AJ. 2006. Microbioreactor arrays with integrated mixers and fluid injectors for high throughput experimentation with pH and dissolved oxygen control. Lab on a Chip 6(9):1229-1235.
19. Ma NN, Koelling KW, Chalmers JJ. 2002. Fabrication and use of a transient contractional flow device to quantify the sensitivity of mammalian and insect cells to hydrodynamic forces. Biotechnol Bioeng 80(4):428-437.
20. Maharbiz MM, Holtz WJ, Howe RT, Keasling JD. 2003. Microbioreactor arrays with parametric control for high-throughput experimentation. Biotechnol Bioeng 85(4):376-381.
21. Maiorella B, Inlow D, Shauger A, Harano D. 1988. Large-scale insect cell-culture for recombinant protein production. Bio/Technology 6:1406-1410.
22. McQueen A, Meilhoc E, Bailey J. 1987. Flow effects on the viability and lysis of suspended mammalian cells. Biotechnol Lett 9:831-836.
23. Mitchell-Logean C, Murhammer DW. 1997. Bioreactor headspace purging reduces dissolved carbon dioxide accumulation in insect cell cultures and enhances cell growth. Biotechnol Prog 13:875-877.
24. Mohamed A-R, editor. 2002. Glycosylation. Dordrecht; Boston: Kluwer Academic.
25. Paul EL, Atiemo-Obeng VA, Kresta SM, editors. 2004. Handbook of industrial mixing: Science and practice. Hoboken: John Wiley & Sons.
26. Perry RH, Green D, editors. 1984. Perry's chemical engineering handbook. Chicago: R.R. Donnelley & Sons Company.
27. Reuveny S, Kim YJ, Kemp CW, Shiloach J. 1993. Effect of temperature and oxygen on cell growth and recombinant protein production in insect cell cultures. Appl Microbiol Biotechnol 38:619-623.
28. Schena M, Shalon D, Davis RW, Brown PO. 1995. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270(20):467-470.
29. Schopf B, Howaldt MW, Bailey JE. 1990. DNA distribution and respiration activity of Spodoptera frugiperda populations infected with wild-type and recombinant Autographa californica nuclear polyhedrosis virus. J Biotechnol 15:169-186.
30. Shuler ML, Kargi F. 2002. Bioprocess engineering: Basic concepts. Upper Saddle River: Prentice Hall PTR.
31. Shuler ML, Wood HA, Granados RR, Hammer DA, editors. 1995. Baculovirus expression systems and biopesticides. New York; Chichester; Brisbane; Toronto; Singapore: Wiley-Liss.
32. Stanbury PF, Whitaker A, Hall S. 1995. Principles of fermentation technology. Oxford; Burlington: Elsevier Science.
33. Szita N, Boccazzi P, Zhang Z, Boyle P, Sinskey AJ, Jensen KF. 2005. Development of a multiplexed microbioreactor system for high-throughput bioprocessing. Lab on a Chip 5(8):819-826.
34. Taticek RA. 1995. Enhanced recombinant protein expression in baculovirus-infected high-density insect cell suspension cultures and the operation of a continuous flow bioreactor. Ithaca: Cornell University.
35. Taticek RA, Shuler ML. 1997. Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system. Biotechnol Bioeng 54:142-152.
36. Taticek RA, Choi C, Phan S-E, Palomares LA, Shuler ML. 2001. Comparison of growth and recombinant protein expression in two different insect cell lines in attached and suspension culture. Biotechnol Prog 17:676-684.
37. Tramper J, Williams JB, Joustra D. 1986. Shear sensitivity of insect cells in suspension. Enzyme Microb Technol 8:33-36.
38. Weiss SA, Orr T, Smith GC, Kalter SS, Vaughn JL, Dougherty EM. 1982. Quantitative measurement of oxygen consumption in insect cell culture infected with polyhedrosis virus. Biotechnol Bioeng 24:1145-1154.
39. Yates JR III, Speicher S, Griffin PR, Hunkapiller T. 1993. Peptide mass maps: A highly informative approach to protein identification. Anal Biochem 214(2):397-408.
40. Zanzotto A, Szita N, Boccazzi P, Lessard P, Sinskey AJ, Jensen KF. 2004. Membrane-aerated microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng 87(2):243-254.
41. Zhang Z, Szita N, Boccazzi P, Sinskey AJ, Jensen KF. 2005. A well-mixed, polymer-based microbioreactor with integrated optical measurement. Biotechnol Bioeng 93(2):286-296.
42. Zhang Z, Boccazzi P, Choi H-G, Perozziello G, Sinskey AJ, Jensen KF. 2006. Microchemostat - Microbial continuous culture in a polymer-based instrumented microbioreactor. Lab on a Chip 6(7):906-913.