Unusual spectroscopic and ligand binding properties of the cytochrome P450-flavodoxin fusion enzyme XplA

Journal of Biological Chemistry

Volumn 287, Issue 23, 2012, Pages 19699-19714

  Bui, Soi H ^a   McLean, Kirsty J ^a   Cheesman, Myles R ^b   Bradley, Justin M ^b   Rigby, Stephen E J ^a   Levy, Colin W ^a   Leys, David ^a   Munro, Andrew W ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; BINDING MODES; COFACTORS; CYTOCHROME P450; EPR SPECTRA; EPR STUDIES; FLAVODOXIN; FUSION ENZYMES; HEME IRON; HIGH AFFINITY; LIGAND BINDING; LIGAND BINDING PROPERTIES; MONOMERIC ENZYMES; MORPHOLINES; RED-SHIFTED; RHODOCOCCUS RHODOCHROUS; SEMIQUINONES; SORET BANDS; SUBSTRATE-FREE; TRINITROBENZENE;

BINDING ENERGY; ENZYME ACTIVITY; LIGANDS; NITROGEN REMOVAL; PORPHYRINS;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

4 PHENYLIMIDAZOLE; CYTOCHROME P450; FLAVODOXIN; HYDROQUINONE; METHIONINE; MORPHOLINE; PROTEIN XPLA; SEMIQUINONE; SERINE; THREONINE; TRIAZENE DERIVATIVE; UNCLASSIFIED DRUG; VALINE;

ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; BINDING SITE; CATALYST; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTALLIZATION; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; LIGHT SCATTERING; NONHUMAN; OXIDATION REDUCTION POTENTIAL; POTENTIOMETRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RHODOCOCCUS; RHODOCOCCUS RHODOCOCCUS; SPECTROSCOPY; ULTRAVIOLET SPECTROSCOPY;

CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME P-450 ENZYME SYSTEM; EXPLOSIVE AGENTS; FLAVODOXIN; IMIDAZOLES; LIGANDS; RHODOCOCCUS; TRIAZINES;

RHODOCOCCUS RHODOCHROUS;

EID: 84861723942     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.319202     Document Type: Article

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