Biodegradation of explosives by transgenic plants expressing pentaerythritol tetranitrate reductase

Nature Biotechnology

Volumn 17, Issue 5, 1999, Pages 491-494

  French, Christopher E ^c   Rosser, Susan J ^a   Davies, Gareth J ^a   Nicklin, Stephen ^b   Bruce, Neil C ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b DEFENCE SCIENCE AND TECHNOLOGY LABORATORY   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Explosives; PETN reductase; Phytoremediation; Transgenic plants

Indexed keywords

AROMATIC NITRO COMPOUND; ESTER; NITRATE; OXIDOREDUCTASE; PENTAERYTHRITYL TETRANITRATE;

ARTICLE; BIODEGRADATION; DENITRIFICATION; ENZYME ACTIVITY; GERMINATION; NONHUMAN; PLANT GROWTH; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOIL POLLUTION; SOIL TREATMENT; TOBACCO; TRANSGENIC PLANT;

BIODEGRADATION, ENVIRONMENTAL; NITROGLYCERIN; OXIDOREDUCTASES; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; TOBACCO; TRINITROTOLUENE;

BACTERIA (MICROORGANISMS); NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0032937445     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/8673     Document Type: Article

References (17)

1. Hoeppel, R.E. & Hinchee, R.E. in Hazardous Waste Site Soil Remediation: Theory and Application of Innovative Technologies (eds Wilson, D.J. & Clarke, A.N.) 311-431 (Marcel Dekker, New York, 1994).
2. Shannon, M.J.R. & Unterman, R. Evaluating bioremediation: distinguishing fact from fiction. Annu. Rev. Microbiol. 47, 715-738 (1993).
3. Cunningham, S.D. & Ow, D.W. Promises and prospects of phytoremediation. Plant Physiol. 110, 715-719 (1996).
4. Raskin, I. Plant genetic engineering may help with environmental cleanup. Proc. Natl. Acad. Sci. USA 93, 3164-3166 (1996).
5. Rugh, C.L., Senecoff, J.F., Meagher, R.B. & Merkte, S.A. Development of transgenic yellow poplar for mercury phytoremediation. Nat. Biotechnol. 16, 925-928 (1998).
6. Binks, P.R., French, C.E., Nicklin, S. & Bruce, N.C. Degradation of pentaerythritol tetranitrate by Enterobacter cloacae PB2. Appl. Environ. Microbiol. 62, 1214-1219 (1996).
7. French, C.E., Nicklin, S. & Bruce, N.C. Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2. J. Bacteriol. 178, 6623-6627 (1996).
8. French, C.E., Nicklin, S. & Bruce, N.C. Aerobic degradation of 2,4,6-trinitrotoluene by Enterobacter cloacae PB2 and by pentaerythritol tetranitrate reductase. Appl. Environ. Microbiol. 64, 2864-2868 (1998).
9. Goel, A., Kumar, G., Payne, G.F. & Dube, S.K. Plant cell biodegradation of a xenobiotic nitrate ester, nitroglycerin. Nat. Biotechnol. 15, 174-177 (1997).
10. Taylor, I.W., Ioannides, C. & Parke, D.V. Organic nitrate reductase: reassessment of its subcellular localization and tissue distribution and its relationship to the glutathione transferases. Int. J. Biochem. 21, 67-71 (1989).
11. Hughes, J.B., Shanks, J., Vanderford, M., Lauritzen, J. & Bhadra, R. Transformation of TNT by aquatic plants and plant tissue cultures. Environ. Sci. Technol. 31, 266-271 (1997).
12. Guerineau, F., Lucy, A. & Mullineaux, P. Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts. Plant Mol. Biol. 18, 815-818 (1992).
13. Cleave, A.P. A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol. Biol. 20, 1203-1207 (1992).
14. Ogawa, N. et al. Purification and characterization of glutathione-independent denitration enzyme of organic nitrate esters in rabbit hepatic cytosol. Biol. Pharm. Bull. 18, 1352-1355 (1995).
15. Schnoor, J.L., Licht, L.A., McCutcheon, S.C., Wolfe, N.L. & Carreira, L.H. Phytoremediation of organic and nutrient contaminants. Environ. Sci. Technol. 29, 318A-323A (1995).
16. Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual 2nd edn. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
17. Horsch, R.B. et al. A simple and general method of transferring genes to plants. Science 227, 1229-1231 (1985).