Synthesis, characterization and preliminary in vitro evaluation of PTH 1-34 loaded chitosan nanoparticles for osteoporosis

Journal of Biomedical Nanotechnology

Volumn 8, Issue 1, 2012, Pages 98-106

  Narayanan, Deepa ^a   Anitha, A ^a   Jayakumar, R ^a   Nair, S V ^a   Chennazhi, K P ^a  

^a AMRITA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Alkaline phosphatase; Chitosan nanoparticles; Cytotoxicity; Osteoporosis; PTH 1 34 peptide delivery

Indexed keywords

AFM; ALKALINE PHOSPHATASE; CHITOSAN NANOPARTICLES; HAEMOLYSIS; IN-VITRO; IN-VITRO EVALUATION; MULTIPLE ROUTES; OSTEOPOROSIS; PARATHYROID HORMONES; PEPTIDE DELIVERY; PEPTIDE RELEASE;

BIOCHEMISTRY; CHITOSAN; COAGULATION; CYTOTOXICITY; DISEASES; GELATION; NANOPARTICLES; PATIENT TREATMENT; PEPTIDES; PHOSPHATASES; ZETA POTENTIAL;

SYNTHESIS (CHEMICAL);

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CHITOSAN; CHITOSAN NANOPARTICLE; NANOPARTICLE; PARATHYROID HORMONE[1-34]; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ATOMIC FORCE MICROSCOPY; CONTROLLED STUDY; DRUG BIOAVAILABILITY; DRUG HALF LIFE; DRUG SYNTHESIS; DYNAMIC LIGHT SCATTERING; EMBRYO; ENZYME LINKED IMMUNOSORBENT ASSAY; GELATION; HEMOLYSIS; HUMAN; HUMAN CELL; IN VITRO STUDY; INFRARED SPECTROSCOPY; LIGHT SCATTERING; MOUSE; NONHUMAN; OSTEOPOROSIS; PH; QUANTITATIVE ANALYSIS; RAT; SCANNING ELECTRON MICROSCOPY; ZETA POTENTIAL;

ALKALINE PHOSPHATASE; ANIMALS; BONE NEOPLASMS; CELL LINE; CELL LINE, TUMOR; CHITOSAN; DRUG CARRIERS; DRUG STABILITY; HEMOLYSIS; HUMANS; L-LACTATE DEHYDROGENASE; MICE; NANOPARTICLES; NIH 3T3 CELLS; OSTEOBLASTS; OSTEOPOROSIS; PARATHYROID HORMONE; PARTICLE SIZE;

EID: 84861470570     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2012.1367     Document Type: Article

References (47)

1. S. Matthew, B. Sharon, and R. J. M. Midura, Parathyroid hormone suppresses osteoblast apoptosis by augmenting DNA repair. Bone 45, 590 (2009).
2. R. L. Jilka, Molecular and cellular mechanisms of the anabolic effect of intermittent PTH. Bone 40, 1434 (2007).
3. S. Lotinun, J. D. Sibonga, and R. T. Turner, Differential effects of intermittent and continuous administration of parathyroid hormone on bone histomorphometry and gene expression. Endocrine 17, 29 (2002).
4. Medication guide Forteo teriparatide rDNA origin injection, Eli lily http://pi.lilly.com/us/forteo-pi.pdf (2010).
5. A. Frank, P. Eric, K.M. Linda, and C. F. Omid, Factors affecting the clearance and biodistribution of polymeric nanoparticles. Mol. Pharmacol. 5, 505 (2008).
6. R. Jayakumar, K. P. Chennazhi, R. A. A. Muzzarelli, H. Tamura, S. V. Nair, and N. Selvamurugan, Chitosan conjugated DNA nanoparticles in gene therapy. Carbohyd. Polym. 79, 1 (2010).
7. R. Jayakumar, D. Menon, K. Manzoor, S. V. Nair, and H. Tamura, Biomedical applications of chitin and chitosan based nanomaterials - A short review. Carbohyd. Polym. 82, 227 (2010).
8. A. Anitha, V. G. Deepagan, V. V. Divya Rani, D. Menon, S. V. Nair, and R. Jayakumar, Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate-chitosan nanoparticles. Carbohyd. Polym. 84, 1158 (2011).
9. A. Anitha, V. V. Divya Rani, R. Krishna, V. Sreeja, N. Selvamurugan, S. V. Nair, H. Tamura, and R. Jayakumar, Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N,O-carboxymethyl chitosan nanoparticles. Carbohyd. Polym. 78, 672 (2009).
10. R. A. A. Muzzarelli, Chitosan composites with inorganic, morphogenetic proteins and stem cells, for bone regeneration. Carbohyd. Polym. 83, 1433 (2011).
11. K. L. Douglas, C. A. Piccirillo, and M. Tabrizian, Effects of alginate inclusion on the vector properties of chitosan-based nanoparticles. J. Control. Release 115, 354 (2006).
12. M. George and T. E. Abraham, Polyionic hydrocolloids for the intestinal delivery of protein drugs: Alginate and chitosan - A review. J. Control. Release 114, 1 (2006).
13. J. M. Dang and K. W. Leong, Natural polymers for gene delivery and tissue engineering. Adv. Drug Deliver. Rev. 58, 487 (2006).
14. W. Ping, H. Xiaoxiao, W. Kemin, T. Weihong, H. Chunmei, and Z. Mingbin, A novel methotrexate delivery system based on chitosan-methotrexate covalently conjugated nanoparticles. J. Biomed. Nanotechnol. 5, 557 (2009).
15. Y. Wenjing, F. Jing, W. Ting, and H. Nongyue, Chitosan/sodium tripolyphosphate nanoparticles: Preparation, characterization and application as drug carrier. J. Biomed. Nanotechnol. 5, 591 (2009).
16. P. Varsha, D. Sheetal, B. Devika, M. Vishal, B. Subhash, and B. L. V. Prasad, Acute and subacute toxicity studies of chitosan reduced gold nanoparticles: A novel carrier for therapeutic agents. J. Biomed. Nanotechnol. 5, 233 (2009).
17. C. Noemi, K. Magnus, F. Eduardo, N. Ramon, R. Ricardo, and J. A. Maria, Ionically crosslinked chitosan nanoparticles as gene delivery systems: Effect of pegylation degree on in vitro and in vivo gene transfer. J. Biomed. Nanotechnol. 5, 162 (2009).
18. H. Nongyue, W. Ting, J. Li, W. Daxin, H. Yan, and Z. Long, Therapy for cerebral ischemic injury with erythropoietin-containing nanoparticles. J. Nanosci. Nanotechnol. 10, 5320 (2010).
19. W. Ting, H.Yan, Z. Long, J. Li, and H. Nongyue, Erythropoietin Nanoparticles: Therapy for cerebral ischemic injury and metabolize in kidney. Nano Biomed. Eng. 2, 31 (2010).
20. M. Nilesh, S. Dinesh, M. Amit, P. Vinayak, I. Ranjit, and D. Durgesh, Biodegradable nanoparticles for targeted delivery in treatment of ulcerative colitis. Adv. Sci. Lett. 4, 349 (2011).
21. S. Aliasgar and D. Dilip, Preparation and evaluation of microemulsion based formulations for rapid-onset intranasal delivery of zonisamide. Adv. Sci. Lett. 3, 442 (2010).
22. F. Jing, W. Daxin, W. Ting, Y. Wenjing, D. Yan, W. Hua, S. Jhiguang, and H. Nongyue, High entrapment efficiency of chitosan/polylactic acid/tripolyphosphate nanosized microcapsules for rapamycin by an emulsion-evaporation approach. J. Biomed. Nanotechnol. 6, 725 (2010).
23. V. M. Bispo, A. A. Mansur, F. Edel, E. F. Barbosa-Stancioli and H. S. Mansur, Biocompatibility of nanostructured chitosan/poly(vinyl alcohol) blends chemically crosslinked with genipin for biomedical applications. J. Biomed. Nanotechnol. 6, 166 (2010).
24. F. C. MacLaughlin, R. J. Mumper, and J. Wang, Chitosan and depolymerised chitosan oligomers as condensing carriers for in vivo plasmid delivery. J. Control. Release 56, 259 (1998).
25. S. Mao, X. Shuai, F. Unger, and M. Simon, The depolymerisation of chitosan: Effects on physicochemical and biological properties. Int. J. Pharm. 281, 45 (2004).
26. F. Zhang-Qi, M. K. Leach, C. Xue-Hui, W. Yi-Chun, T. Tian, S. Xiao-Lei, D. Yi-Tao, and G. Zhong-Ze, Electrospun chitosan nanofibers for hepatocyte culture. J. Biomed. Nanotechnol. 6, 658 (2010).
27. Q. Gan and T. Wang, Chitosan nanoparticle as protein delivery carrier-systematic examination of fabrication conditions for efficient loading and release. Colloid. Surface B 59, 24 (2007).
28. Y. Pan, Y. Li, and H. Zhao, Bio adhesive polysaccharide in protein delivery system: Chitosan nanoparticles improve the intestinal absorption of insulin in vivo. Int. J. Pharm. 249, 139 (2002).
29. Y. Xu and Y. Du, Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. Int. J. Pharm. 250, 215 (2003).
30. L. Yi-Sheng, O. Yoshiharu, and M. Saburo, Effects of chitosancarboxymethyl dextran nanoparticles on cell proliferation and on serum cytokine regulation. J. Biomed. Nanotechnol. 6, 247 (2010).
31. Y. Wenjing, F. Jing, W. Daxin, W. Ting, W. Hua, J. Shiguang, and H. Nongyue, Study on chitosan/polycaprolactone blending vascular scaffolds by electrospinning. J. Biomed. Nanotechnol. 6, 254 (2010).
32. W. Ting, F. Zhangqi, H. Nongyue, W. Zhifei, L. Song, G. Yafei, and X. Lijian, A novel preparation of nanocapsules from alginateoligochitosan. J. Nanosci. Nanotechnol. 7, 4571 (2007).
33. V. Imran, S. Can, and S. O. Senem, Chitosan coated furosemide liposomes for improved bioavailability. J. Biomed. Nanotechnol. 7, 426 (2011).
34. A. S. Mohammad, M. Fatemeh, M. Vahid, and F. Mehdi, Fabrication of porous chitosan/poly(vinyl alcohol) reinforced single-walled carbon nanotube nanocomposites for neural tissue engineering. J. Biomed. Nanotechnol. 7, 276 (2011).
35. C. N. Ramesh, P. N. Singh, S. Kant, P. Maiti, and J. K. Pandit, Chitosan coated PLA nanoparticles for ophthalmic delivery: Characterization, in-vitro and in-vivo study in rabbit eye. J. Biomed. Nanotechnol. 6, 648 (2010).
36. B. S. Andreas, Chitosan and its derivatives: Potential excipients for peroral peptide delivery systems. Int. J. Pharm. 194, 1 (2000).
37. T. Jung, W. Kamm, A. Breitenbach, E. Kaiserling, J. X. Xiao, and T. Kissel, Biodegradable nanoparticles for oral delivery of peptides: Is there a role for polymers to affect mucosal uptake. Eur. J. Pharmaceut. Biopharmaceut. 50, 147 (2000).
38. M. M. Leanes, R. Nankervis, A. Smith, and L. Illum, Use of the ninhydrin assay to measure the release of chitosan from oral solid dosage forms. Int. J. Pharm. 271, 241 (2004).
39. S. Senel, G. Ikinci, S. Kas, A. Yousefi-Rad, and A. A. Hincal, Chitosan films and hydrogels of chlorhexidine gluconate for oral mucosal delivery. Int. J. Pharm. 193, 197 (2000).
40. S. Senel, M. J. Kremer, S. Kas, P. W. Wertz, A. A. Hincal, and C. A. Squier, Enhancing effect of chitosan on peptide drug delivery across buccal mucosa. Biomaterials 21, 2067 (2000).
41. J. Smith, E. Wood, and M. Dornish, Effect of chitosan on epithelial cell tight junctions. Pharm. Res. 21, 43 (2004).
42. O. Makoto, F. Sebastien, P. V. Jean, L. B. Mary, T. P. John, Jr., and J. Thomas Gardella, Prolonged signaling at the parathyroid hormone receptor by peptide ligands targeted to a specific receptor conformation. Proc. Natl. Acad. Sci. USA 105, 16525 (2008).
43. C. M. Weaver, M. Peacock, B. R. Martin, G. P. McCabe, J. Zhao, D. L. Smith, and M. E. Wastney, Quantification of biochemical markers of bone turnover by kinetic measures of bone formation and resorption in young healthy females. J. Bone Miner. Res. 12, 1714 (1997).
44. Rodney Boyer in Modern Experimental Biochemistry, 3rd edn., Dorling Kinderlsley, India (2007), Chap. 2.
45. N. Sanoj Rejinold, M. Muthunarayanan, K. Muthuchelian, K. P. Chennazhi, S. V. Nair, and R. Jayakumar, Saponin-loaded chitosan nanoparticles and their cytotoxicity to cancer cell lines in vitro. Carbohyd. Polym. 84, 407 (2011).
46. K. E. S. Poole, and R. Jonathan, Gastrointestinal/Endocrine and metabolic diseases Parathyroid hormone-a bone anabolic and catabolic agent. Curr. Opin. Pharmacol. 5, 612 (2005).
47. Z. Hong-liang, W. Si-Hui, T. Yi, Z. Lin-quan, and S. Zhengquan, Preparation and characterization of water-soluble chitosan nanoparticles as protein delivery system. J. Nanomater. 10, 4 (2010).