Rapid and specific detection of Escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification

Applied and Environmental Microbiology

Volumn 78, Issue 8, 2012, Pages 2727-2736

  Wang, Fei ^a   Jiang, Lin ^a   Yang, Qianru ^a   Prinyawiwatkul, Witoon ^a   Ge, Beilei ^a,b  

^a LOUISIANA STATE UNIVERSITY AGRICULTURAL CENTER   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL STRAINS; CELL NUMBER; ESCHERICHIA COLI O157; FOOD SAMPLES; GROUND BEEF; LIMITS OF DETECTION; LINEAR RELATIONSHIPS; LOW LEVEL; PURE CULTURE; REAL-TIME QUANTITATIVE PCR; RELIABLE DETECTION; SAMPLE TESTING; SEROGROUPS; SHIGA TOXIN-PRODUCING E. COLI; SPECIFIC DETECTION; STANDARD CURVES;

ASSAYS; CELL CULTURE; ESCHERICHIA COLI; GENES; ISOTHERMS; POLYMERASE CHAIN REACTION; TURBIDITY;

MEATS;

BACTERIAL DNA; PRIMER DNA;

BIOASSAY; COLIFORM BACTERIUM; POLYMERASE CHAIN REACTION; REAL TIME; SAMPLING; TOXIN; TURBIDITY;

ARTICLE; BACTERIAL GENE; CLASSIFICATION; COMPARATIVE STUDY; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; MEAT; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; UNITED STATES; VEGETABLE;

BACTERIOLOGICAL TECHNIQUES; DNA PRIMERS; DNA, BACTERIAL; GENES, BACTERIAL; MEAT; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SENSITIVITY AND SPECIFICITY; SHIGA-TOXIGENIC ESCHERICHIA COLI; UNITED STATES; VEGETABLES;

UNITED STATES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTUCA; SPINACIA OLERACEA;

EID: 84861162176     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07975-11     Document Type: Article

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