O serogroup specific real time PCR assays for the detection and identification of nine clinically relevant non-O157 STECs

Food Microbiology

Volumn 28, Issue 3, 2011, Pages 478-483

  Lin, Andrew ^a   Sultan, Omar ^b   Lau, Henry K ^a   Wong, Evelyn ^a   Hartman, Gary ^a   Lauzon, Carol R ^b  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b CALIFORNIA STATE UNIVERSITY   (United States)

Author keywords

Escherichia coli surveillance; Foodborne disease; Shiga toxin

Indexed keywords

SHIGA TOXIN;

ARTICLE; BACTERIAL COUNT; BIOSYNTHESIS; CLASSIFICATION; FOOD CONTAMINATION; FOOD CONTROL; GENE AMPLIFICATION; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHYLOGENY; PRODUCT SAFETY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEROTYPING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SPECIES DIFFERENCE;

COLONY COUNT, MICROBIAL; CONSUMER PRODUCT SAFETY; FOOD CONTAMINATION; FOOD MICROBIOLOGY; GENE AMPLIFICATION; HUMANS; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEROTYPING; SHIGA TOXIN; SHIGA-TOXIGENIC ESCHERICHIA COLI; SPECIES SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTUCA; MALUS X DOMESTICA;

EID: 79952007902     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2010.10.007     Document Type: Article

References (33)

1. Ballmer K., Korczak B.M., Kuhnert P., Slickers P., Ehricht R., Hächler H. Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray. Journal of Clinical Microbiology 2007, 45:370-379.
2. Bettelheim K.A. Non-O157 verotoxin-producing Escherichia coli: a problem, paradox, and paradigm. Experimental Biology and Medicine 2003, 228:333-344.
3. Bettelheim K.A. The non-O157 Shiga-toxigenic (verocytotoxigenic) Escherichia coli; under-rated pathogens. Critical Reviews in Microbiology 2007, 33:67-87.
4. Beutin L. Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health 2006, 53:299-305.
5. Beutin L., Jahn S., Fach F. Evaluation of the 'GeneDisc' real-time PCR system for detection of enterohaemorrhagic Escherichia coli (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and H-antigen-associated genes. Journal of Applied Microbiology 2009, 106:1122-1132.
6. Brooks J.T., Sowers E.F., Wells J.G., Greene K.D., Griffin P.M., Hoekstra R.M., Strockbine N.A. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. Journal of Infectious Diseases 2005, 192:1422-1429.
7. Catarame T.M., O'Hanlon K.A., Duffy G., Sheridan J.J., Blair I.S., McDowell D.A. Optimization of enrichment and plating procedures for the recovery of Escherichia coli O111 and O26 from minced beef. Journal of Applied Microbiology 2003, 95:949-957.
8. Chart H., Willshaw G.A., Cheasty T. Evaluation of a reversed passive latex agglutination test for the detection of verocytotoxin (VT) expressed by strains of VT-producing Escherichia coli. Letters in Applied Microbiology 2001, 32:370-374.
9. Cohen N.J., Jones R.C., Schermond J.S., Gerber S.I. Identifying Shiga toxin-producing Escherichia coli: results of a laboratory evaluation. Foodborne Pathogens and Disease 2008, 5:295-298.
10. Durso L.M., Bono J.L., Keen J.E. Molecular serotyping of Escherichia coli O26:H11. Applied and Environmental Microbiology 2005, 71:4941-4944.
11. Feng L., Senchenkova S.N., Tao J., Shashkov A.S., Liu B., Shevelev S.D., Reeves P.R., Xu J., Knirel Y.A., Wang L. Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay. Journal of Bacteriology 2005, 187:758-764.
12. Feng P., Weagant S.D. Diarrheagenic Escherichia coli. Bacteriological Analytical Manual 2009, Chapter 4a. http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM070080.
13. Fratamico P.M., DebRoy C., Strobaugh T.P., Chen C.Y. DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes. Canadian Journal of Microbiology 2005, 51:515-522.
14. Fratamico P.M., DebRoy C., Miyamoto T., Liu Y. PCR Detection of enterohemorrhagic Escherichia coli O145 in food by targeting genes in the E. coli O145 O-antigen gene cluster and the Shiga toxin 1 and Shiga toxin 2 genes. Foodborne Pathogens and Disease 2009, 6:605-611.
15. Gilmour M.W., Chui L., Chiu T., Tracz D.M., Hagedorn K., Tschetter L., Tabor H., Ng L.K., Loui M. Isolation and detection of Shiga toxin-producing Escherichia coli in clinical stool samples using conventional and molecular methods. Journal of Medical Microbiology 2009, 58:905-911.
16. Gioffré A., Meichtri L., Zumárraga M., Rodríguez R., Cataldi A. Evaluation of a QIAmp DNA stool purification kit for Shiga-toxigenic Escherichia coli detection in bovine fecal swabs by PCR. Revista Argentina De Microbiologia 2004, 36:1-5.
17. Gould L.H., Bopp C., Strockbine N., Atkinson R., Baselski V., Body B., Carey R., Crandall C., Hurd S., Kaplan R., Neill M., Shea S., Somsel P., Tobin-D'Angelo M., Griffin P.M., Gerner-Smidt P. Recommendations for diagnosis of Shiga toxin-producing Escherichia coli infections by clinical laboratories. Morbidity and Mortality Weekly Report Recommendations and Reports 2009, 58:1-14.
18. Griffin P.M., Tauxe R.V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiologic Reviews 1991, 13:60-98.
19. Gyles C.L. Shiga toxin-producing Escherichia coli: an overview. Journal of Animal Science 2007, 85:E45-E62.
20. Hiramatsu R., Matsumoto M., Miwa Y., Suzuki Y., Saito M., Miyazaki Y. Characterization of Shiga toxin-producing Escherichia coli O26 strains and establishment of selective isolation media for these strains. Journal of Clinical Microbiology 2002, 40:922-925.
21. Johnson K.E., Thorpe C.M., Sears C.L. The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clinical Infectious Diseases 2006, 43:1587-1595.
22. Karmali M.A. Infection by Shiga toxin-producing Escherichia coli: an overview. Molecular Biotechnology 2004, 26:117-122.
23. Levine M.M., Xu J.G., Kaper J.B., Lior H., Prado V., Tall B., Nataro J., Karch H., Wachsmuth K. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. Journal of Infectious Diseases 1987, 156:175-182.
24. March S.B., Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with haemorrhagic colitis. Journal of Clinical Microbiology 1986, 23:869-872.
25. Murphy N.M., McLauchlin J., Ohai C., Grant K.A. Construction and evaluation of a microbiological positive process internal control for PCR-based examination of food samples for Listeria monocytogenes and Salmonella enterica. International Journal of Food Microbiology 2007, 120:110-119.
26. O'Hanlon K.A., Catarame T.M.G., Duffy G., Blair I.S., McDowell D.A. RAPID detection and quantification of E. coli O157/O26/O111 in minced beef by real-time PCR. Journal of Applied Microbiology 2004, 96:1013-1023.
27. Paton A.W., Paton J.C. Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113. Infection and Immunity 1999, 67:5930-5937.
28. Perelle S., Dilasser F., Grout J., Fach P. Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test. Journal of Applied Microbiology 2002, 93:758-764.
29. Perelle S., Dilasser F., Grout J., Fach P. Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction. Journal of Applied Microbiology 2005, 98:1162-1168.
30. Perelle S., Dilasser F., Grout J., Fach P. Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157. International Journal of Food Microbiology 2007, 113:284-288.
31. Shooter R.A., Cooke E.M., Rousseau S.A., Breaden A.L. Animal sources of common serotypes of Escherichia coli in the food of hospital patients. Possible significance in urinary-tract infections. Lancet 1970, 2:226-228.
32. STEC Center National Food Safety & Toxicology Center 2009, Michigan State University. http://www.shigatox.net/stec/cgi-bin/index.
33. Wang L., Curd H., Qu W., Reeves P.R. Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes. Journal of Clinical Microbiology 1998, 36:3182-3187.